白豌豆 的英文怎麼說
中文拼音 [báiwāndòu]
白豌豆
英文
white peas-
These results suggested that high litre antiserum had been acquired. 5. immunoassay of plant ferritin
這表明通過上述方法制備的豌豆鐵蛋白抗血清具有較高的效價。The data above showed that algae phycobiliprotein fluorescent probes not only facilitate the study to pea actins but also provide a doable method to label other plant proteins. it has a good foreground for algae fluorescence probe to be used in the immunity fluorescent detection of plant cells. the gene clon
對豌豆肌動蛋白藻熒光探針的初步研究，不僅為豌豆肌動蛋白的免疫熒光檢測提供了便利條件，而且通過改變抗體，則可以將藻熒光探針用於其他植物蛋白的標記，藻熒光探針優越的熒光特性使其在植物材料的免疫熒光檢測中具有廣闊的應用前景。However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting
利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。Subjected to page under non - denaturating conditions, the purified pea ferritin migrated was single band, which indicates that the purified ferritin of pea has attained electrophoresising purity
Sds - page顯示該蛋白僅含28kd ?條亞基，表明純化得到的豌豆鐵蛋白具有完整的亞基。. from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體，從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白，將兩者偶聯制備的藻熒光探針，不僅保持了藻膽蛋白很強的抗熒光淬滅能力，而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。In this paper, the pea ferritin was purified from pea seed, and its antiserum was raised from rabbits for investigating the expression of transgenic plant. the main experimental results were summarized as following : 1. establishment of ferritin detection system
本論文開展了豌豆鐵蛋白的純化、免疫家兔制備豌豆鐵蛋白抗血清以及植物鐵蛋白免疫學檢測等一系列的研究，現將主要研究內容和結果總結如下： 1 、鐵蛋白檢測系統的建立純化過程中以鄰菲咯啉顯色法檢測鐵蛋白，具有簡單、靈敏、直觀等優點。We are a manufacture and exporter that specialized in frozen and fresh vegetables and fruits. the main product include frozen mixed vegetables, frozen green peas, green beans, sweet corn, carrot, broccli cauliflower, onion, cabbage, pea pods, sugar snap peas, strawberry, white peach, yellow peach, chestnut etc
唐山鮮鮮食品有限公司是一家專業出口農副產品的企業，公司主要經營的產品有：混合菜、板栗、青豆、青刀豆、玉米、胡蘿卜、白蘿卜、西蘭花、洋蔥、包菜、 、荷蘭豆、甜豌豆、草莓、白桃、黃桃、水產品等。Under the conditions of sds - page, it depolymerized into only one subunit of 28 kd, which reveal that the subunit of purified ferritin of pea is undivided 4. preparation of antiserum of pea ferritin the purified pea ferritin was mixed with freund ' s complete adjuvant or freund ' s incomplete adjuvant, and then administered through palmula, back, abdominal cavity, vena auricularis and other routes for rabbits. seven weeks later, the litre of antiserum reached 1 i 32
4 、免疫家兔制備豌豆鐵蛋白抗血清民二功尋咬徑b一侖一以純化的豌豆鐵蛋白作為抗原，並分別加入弗氏完全佐劑和弗氏不完全佐劑等兔疫家兔，經過足掌、背部皮下、腹腔和耳靜脈等途徑進行免疫， 7周后獲得效價高達1 ： 32以上的豌豆鐵蛋白抗血清。Sequence analysis showed that the full length of this cdna which encodes 364 amino acids is 1398bp. it has 71 % and 69 % identities to lycopersicon esculentum and pisum sativum respectively in amino acid level. this gene is a membrane protein which has one signal peptide, seven transmembrane helices, three n - glycosylation sites and one o - glycosylation site
序列分析表明，該基因的cdna全長為1398bp ，開放閱讀框為1095bp ，編碼一個364個氨基酸的多肽，與番茄和豌豆中的該家族基因分別具有71和69的氨基酸同源性，是一種膜蛋白，具有1個信號肽序列， 7個跨膜螺旋， 3個n ?糖基化位點和1個o ?糖基化位點，分子量大約為39 . 174kd ，等電點為8 . 07 。The afcd also completed studies on intensive greenhouse production for local adaptation. in addition, two new vegetable varieties suitable for local production ? white bitter cucumber and garden pea ? were introduced
此外，該署已完成環控溫室作物生產的本地適應性研究，並引進兩個適合本地種植的新蔬菜品種，即白玉苦瓜和軟莢豌豆。The crude extraction of dry pea seed, which was obtained through marination, homogenization, filtration, centrifugation and other methods, was precipitated by 50 mmol / l ( final concentration ) mgq2. the pellet was chromatographed on aca ^ gel filtration and deae - cellulose 52 ( de 52 ) anion - exchanger. single eluting peak containing ferritin was obtained finally
2 、豌豆種子鐵蛋白的純化豌豆種子經浸泡、勻漿、過濾、離心等操作得到豌豆鐵蛋白粗提液，再用終濃度為50mmol / l的mgcl _ 2鹽析等處理后，經aca _ （ 22 ）凝膠過濾柱層析和deae -纖維素52 （ de52 ）陰離子交換柱層析等方法進行純化，得到單一的豌豆鐵蛋白洗脫峰。By gene fusion and prokaryotic expression, we purified a pea actin isoform ( peac1 ), his - tagged peac1, his - tagged gfp and his - tagged peac1 - gfp from inclusion body. after filtrating a series of induction condition, we expressed and purified his - tagged peac1 with soluble form in a large amount
利用基因融合技術，原核表達並從包涵體中純化了豌豆肌動蛋白異型體peac1 、 his - taggedpeac1 、 his - taggedgfp以及his - taggedpeac1 - gfp ，並通過誘導條件的篩選達到了可溶性表達與大量純化his - taggedpeac1 - gfp的目的。He started with the sky and later described how “ very gingerly i mixed a little blue paint on the palette, and then with infinite precaution made a mark about as big as a bean upon the affronted snow - white shield
他先從天空畫起，后來他描述如何「非常謹慎地在調色板上加入一點兒藍色調，然後以萬分的小心，在這塊被蓄意冒犯的雪白的防護板上點上豌豆大的一筆。Using agarose gel double diffusion method, the antiserum of pea ferritin can cross - react with the crude extraction of soybean seed ferritin. the result indicated that high specific antiserum had been acquired and could be used ~ in the detection of the expressi
5 、植物鐵蛋白的免疫學檢測瓊脂糖免疫雙相擴散法顯示，制備的豌豆鐵蛋白抗血清可與大豆鐵蛋白粗提物發生免疫交叉反應。After manipulated as above, the productive rate of purified pea ferritin is 20 - 30mg / kg
通過上述操作，豌豆鐵蛋白的產率為20 - 30mg kg 。分享友人