盒基因 的英文怎麼說

中文拼音 [héjīyīn]
盒基因 英文
box gene
  • 盒 : 名詞（盛東西的器具） box; case
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  1. Cloning of ctsox2 gene shows the validation of rpgw - pcr used for isolating genes from the digested genomic dna

    C心以2基因的hmg盒區外的序列信息可以進一步研究它的功育旨。
  2. , as48 was identified to be the mouse sox4 homologue. ts47 may be a sox21 orthologue of trionyx sinensis, and, as47 may be a sox4 orthologue of alligator sinensis based on blast search. blast analyses showed that the percentage of identity at the amino acid level in the hmg - box region between ts - 41, - 42, as - 41, - 42 and the non - reptilian sox - 1, - 2, - 3 was lower than that among the non - reptilian sox - l, - 2, - 3

    同源序列比較表明： as43 46和ts43 46與鼠sox12的hmg盒區有95的氨基酸同源性，表明是鼠sox12的同源基因（ homologues ） ： as47 as48與鼠sox4的hmg盒區分別有100和97的氨基酸同源性，可以認為as47 48是鼠sox4的同源基因；與鼠、鳥sox21的hmg盒區相比， ts47分別有98和100的氨基酸同源性。
  3. Cloning and sequence analysis of apetala1 homologous gene from cultivated and wild loquat

    盒基因的克隆和表達研究
  4. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  5. He had short time training course in university of vrije of brussels and nih on homeobox gene lim family study. main fields : neural protection and regeneration. special research interests : neurotrophic factors

    近年來，著重於1神經營養因子2同源盒基因lim家族3低氧等所致腦損傷機制及其干預措施4神經肌肉乾細胞發育分化與組織工程等方面的研究。
  6. Gene cassette could be integrated into an integron with recombination between atti and attc sites by integrase ( inti ), or excised from an integron and become a free circle molecule

    基因盒可從整合子中切除成為游離的環狀分子，或者將外源基因盒捕獲並整合入整合子。
  7. Integron consists of an integrase gene ( inti ), a integrase specific recombination site ( atti ), and gene cassettes with varied number

    整合子由整合酶（ integrase ）基因（ intl ） 、整合酶特異性重組點atti和數目不定的基因盒（ genecassette ）組成。
  8. In two of these isolates with typical class 1 integron, it was showed that the integrons were both harbored in plasmids after intll southenbloting both on their genome dna and plasmid dna

    1株福氏志賀菌y變種攜帶的基因盒類型與其中一株宋內志賀菌相同，為護漢17和aad減萬。
  9. 1. characteristic of typical class 1 integron typical class 1 integron includes 5 " conserved region ( inti1 ), varied region ( gene cassettes ), and 3 " conserved region ( qace 1 and sull )

    典型類整合子的分佈及一級結構特徵典型的類整合子包括有5 』保守片段（ inti1 ） 、可變基因盒區（引物inf inb ）和3 』保守片段（引物qace 1 sul1 ） 。
  10. Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods

    大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。
  11. Staphylococcal cassette chromosome mec, sccmec

    基因的葡萄球菌盒式染色體
  12. A aada2 cassette, encoding aminoglycoside adenylyltransferase, was found in 2 isolates of shigella sonnei, and a two cassettes array of dfra1, encoding dihydrofolate reductase, and aadala in one isolate of shigella sonnei, and another two cassettes array of dfra1 7 and aada5 in two isolates of shigella sonnei and shigella flexneri y - variant each, and a dfrv cassette in one isolate of shigella flexneri 6, respectively

    對其可變區測序分析，其中2株宋內志賀菌i類整合子均攜帶1個基因盒，為氨基糖昔腺營酞基轉移酶（ aminoglycosideadenylyltransferase ） az基因（ aa朔2 ） ，編碼對鏈黴素的抗性。
  13. Three kinds of integron were detected from 7 s. typhimurium with a size of 638bp, 1913bp and 2013bp respectively. the products were cloned and sequenced. the results showed that they contained two aada2 gene, one dhfrxii gene associated with the resistance

    序列分析表明，這7株鼠傷寒沙門氏菌的型整合子可分為三種類型，一種大小為638bp ，存在1個閱讀框；另一種大小為1913bp ，含1個aada2 、 1個dhfr基因盒和1個閱讀框；第三種大小為2013bp ，有1個aada2基因盒和1個閱讀框。
  14. Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation

    具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。
  15. We amplified ppsa and tkta from e. coli k - 12 by pcr and constructed recombinant plasmids of them in pbv220 vector containing prpl promoter. because of each gene carrying pl promoter, four productions of ligation were obtained, that are ppt -, ppt -, ptp -, and ptp -. the results of sds - page demonstrated that the bands at 84kd and 73kd were more intensive than the same ones of the controls

    3 .從大腸桿菌中克隆了莽草酸途徑關鍵酶基因aro刁、 aroc 、 arob及整體代謝調控基因csra及其側翼序列;從質粒pet28a中克隆了kan抗性基因; 4 .構建了基因盒子aroaar口carobkan ，在其前面加入了強啟動子ptac ，初步證明了pta 。
  16. It can be futher verified by the test result that the pstvd found in the northeast region of china may be introduced into china by following the introduction of the potato cultivar irish cobbler to various countries and irish cobbler was imported in heilongjiang province prior to the 1960s

    在國內，首次對中國分離株系進行cdna基因克隆和序列分析，進一步驗證了中國株系的由來及其結構特點，對于中國在pstvd檢測水平的提高、檢測試劑盒制備、 pstvd的防治及其致病機理、轉基因育種等提供重要依據。
  17. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  18. Ln our experiment, a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos, the fl sequence and kozak sequence for improving the genetic statement in the level of translation, were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process, and the products were linked into pbil21

    本實驗中人工合成了美洲擬鰈抗凍蛋白基因，並在美洲擬鰈抗凍蛋白基因兩側添加了許多調控序列，如具有加倍增強子的35s啟動子和nos終止子，它們可以促進抗凍蛋白基因的轉錄，及可在翻譯水平上提高基因表達的序列和kozak序列，從而構建了抗凍蛋白基因的高效表達盒。
  19. The fda has recently approved a genetic test ( invader ugt1a1, made by third wave technologies, inc. ) to aid in the detection and identification of mutations in the ugt1a1 * 28 allele

    2005年8月fda批準了一個由第三波科技公司（好眼熟的名字）生活的基因檢測試劑盒用於檢測ugt1a1 * 28基因型。
  20. One gene cassette usually contains a open reading frame encoding antibiotic resistance and a specific recombination site called attc ( or 59 - base element )

    基因盒往往由一個編碼抗生素抗性的開放讀碼框（ orf ）和一個整合位點attc組成。
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