目標酶 的英文怎麼說

中文拼音 [mùbiāo]
目標酶 英文
target enzyme
  • 目 : Ⅰ名詞1 （眼睛） eye 2 （大項中再分的小項） item 3 [生物學] （把同一綱的生物按彼此相似的特徵分為幾...
  • 標 : Ⅰ名詞1 [書面語] （樹梢） treetop; the tip of a tree2 （枝節或表面） symptom; outside appearance; ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 目標 : 1. （對象） target; objective 2. （目的） goal; aim; destination
  1. Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr

    由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。
  2. So it plays an important role and demonstrates its unique advantages over other soil animals in the assessment of heavy metal contamination of environment ; this review first briefly summarizes some methodological systems and major parameters ( community structures, species character, survival, growth, reproduction, metallothionein, and enzyme ) used in the study of ecotoxicology and other related biomarkers in applying collembola in ecological risk assessment of polluted soils

    本文簡要概述彈尾目昆蟲在污染土壤生態風險評估中、生態毒理學研究以及其他相關生物標志物研究上的一些方法體系及檢測主要指標參數（群落結構，種群特徵，生存率，生長率，繁殖率，金屬硫蛋白和酶活指標） 。
  3. The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene. the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it

    豇豆胰蛋白酶抑制劑（ cpti ）基因是目前在植物抗蟲基因工程中應用僅次於bt基因的廣譜性抗蟲基因。鑒於它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。
  4. Cyclooxygenase ( cox ) is an important target for treating pain and inflammation, but off - target effects of known cox inhibitors have wreaked havoc on their clinical value

    環氧合酶（ cox ）是一個重要指標，為治療疼痛和炎癥，但非目標效應已知考克斯抑制劑肆虐就其臨床應用價值
  5. P - galactosidase of e. coli which is scarce in human blood plasma and has a variety of substrates, is one of the most frequently used enzyme labels. it is used both in heterogeneous and homogeneous enzyme immunoassay

    大腸桿菌的-半乳糖苷酶，因人血漿中缺乏此酶，並具有大量易得的底物，被用於均相及非均相免疫分析，是目前最常用的標記酶之一。
  6. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  7. It was proved that the amount of immobilized antibody and the immunoactivity of bound antibodies could be well improved by colloidal au. hrp labeled antibody reacted with antigen, then hrp biocatalyzed dab ( 3, 3 ’ - diaminobenzidine ) in the presence of h _ 2o _ 2, resulting in an insoluble product onto the electrode surface, to achieve an obviously decreased frequency

    在h _ 2o _ 2存在下，通過標記在抗人igg抗體上的辣根過氧化物酶（ hrp ）催化底物3 ， 3 』 -聯苯二胺（ dab ） ，反應生成不溶性產物沉積到石英晶振的au電極表面，達到質量放大的目的。
  8. But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area ". to answer these questions, we observed systemically the synaptic connections among the pv - like immunoreactive neurons, fibers and terminals and the connections between dnpi - like, gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry, triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique

    但是目前對新發現的囊泡膜mu轉運體一dnn樣陽性終末與帶狀區內pag樣陽性神經元之間ej關系，以及谷氨酸脫發酶（ gad ，是gaba能神經元和終末的特異性標識物）是否參與其調控作用，尚缺乏系統的形態學資料。
  9. Abstract : biological invasions are a continuous feature of a non - equilibrium world, ever more so as a result of accidental and deliberate introductions by mankind. while many of these introductions are apparently harmless, others have significant consequences for organisms native to the invaded range, and entire communities may be affected. here we provide a survey of common models of range expansion, and outline the consequences these models have for patterns in genetic diversity and population structure. we describe how patterns of genetic diversity at a range of markers can be used to infer invasion routes, and to reveal the roles of selection and drift in shaping population genetic patterns that accompany range expansion. we summarise a growing range of population genetic techniques that allow large changes in population size ( bottlenecks and population expansions ) to be inferred over a range of timescales. finally, we illustrate some of the approaches described using data for a suite of invasions by oak gallwasps ( hymenoptera, cynipidae, cynipini ) in europe. we show that over timescales ranging from 500 10000 years, allele frequency data for polymorphic allozymes reveal ( a ) a consistent loss of genetic diversity along invasion routes, confirming the role of glacial refugia as centres of genetic diversity over these timescales, and ( b ) that populations in the invaded range are more subdivided genetically than those in the native range of each species. this spatial variation in population structure may be the result of variation in the patchiness of resources exploited by gallwasps, particularly host oak plants

    文摘:生物入侵是不均衡世界的一個永恆話題，尤其是當人類有意或無意地引入物種后.很多引入顯然是無害的，但另外一些則有著嚴重的後果，會給入侵地的生物以至於整個生物群落造成影響.本文總結了分佈區擴張的常見模式，概述了它們對遺傳多樣性和種群結構式樣所造成的影響.描述了如何根據以一批遺傳標記所得到的遺傳多樣性式樣來推斷入侵途徑，來揭示伴隨擴張選擇和漂變在形成種群遺傳樣式中的作用.本文對日益增多的群體遺傳學方法進行了總結，這些技術可以用來在不同的時間尺度上推斷種群規模所發生的巨大變化（瓶頸效應及種群擴張） .最後，我們以歐洲櫟癭蜂（膜翅目，癭蜂科，癭蜂族）一系列入侵的數據為例對一些方法進行了說明.從500 10000年的時間尺度上，多態的等位酶位點上等位基因頻率的數據表明: 1 ）遺傳多樣性沿入侵路線呈不斷下降的趨勢，支持了冰河期避難所作為遺傳多樣性中心的作用; 2 ）入侵地區的種群與該物種原產地的種群相比，遺傳上的分化更為強烈.這種種群結構在空間上的變異可能是被櫟癭蜂開發的資源尤其是櫟樹寄主在斑塊上出現變異的反映
  10. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  11. Now, tamoxifen is still the standard endocrine therapeutic drug or the premenopausal patients, but aromatase inhibitors can bring more benefit for the postmenopausal patients

    目前，三苯氧胺對絕經前患者仍是內分泌治療的標準用藥，但對絕經後患者應用芳香化酶抑制劑會有更大的效益。
  12. The studies were aimed at optimizing ph and ionic strength and the size of the digestion element, to produce maximal protein digestion with minimal effects on chromatographic integrity

    研究的目標是優化ph 、離子強度和酶解元素的尺寸，以產生最大的蛋白酶解產物而對整個色譜的影響最小。
  13. Detailed illness reachs advisory goal : i was done 3 times recently " second liver two half - and - half " and " liver function ", " second liver two half - and - half " each index is negative, cereal third transaminase is not stable, other index is normal

    具體病情及咨詢目的：我最近做了三次「乙肝兩對半」和「肝功能」 ， 「乙肝兩對半」的各項指標都為陰性，谷丙轉氨酶不穩定，其它指標都正常。
  14. Objective : to study the practical value of testing the telomerase activity from colorectal cancinomas specimens

    摘要目的：探討端粒酶活性檢測在大腸癌活檢標本中的應用價值。
  15. At present, by the immunohistochemical techniques, there have been the expressions of hsp70, bfgf, cox2 after rat cerebral contusion. the consequences show that the expressions of these three indications show certain changing regulations after rat brain contusion. but there has not been any report about the application of these indications on the estimation of the aging on human cerebral contusion

    目前，已有人分別應用免疫組織化學技術對熱休克蛋白70 （ hsp70 ） 、堿性成纖維細胞生長因子（ bfgf ）和環氧合酶- 2 （ cox2 ）在大鼠腦挫傷后的表達進行了研究，結果表明這三項指標的表達在傷后均呈現出一定的變化規律，但未見有人將hsp70 、 bfgf和cox2應用於人腦挫傷時間推斷的研究。
  16. Tyrosine kinases are attractive as targets in the research of therapeutic agents, not only against cancer, hut also against many other diseases

    在包括癌癥和其他許多疾病的治療研究中，酪氨酸激酶都是研究的目標和對象。
  17. To prove that the cloned dna fragment can express tryptopanase, a new plasmid pet28c - tnaa, in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ), a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter, which is inducible by iptg

    用iptg誘導表達t7rna聚合酶，以表達質粒上的目的基因。在葡萄糖存在的條件下，用常規方法發酵和誘導（ 37 1mmiptg ） ，發現表達的蛋白質條帶的分子量與理論上計算的分子量一致。但是發酵液中檢測不到吲哚，表明雖然表達了目標蛋白，但表達的蛋白質沒有酶活性。
  18. The positive transformants with the integrates mn - sod gene and cuzn - sod gene were identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein and cuzn - sod protein was successfully expressed in pichia pastoris based on the evidences that the obvious activity of sod existed in native - page and enzymatic activity test

    Pcr鑒定進一步說明，目標基因已經重組到宿主基因組染色體上； 0 . 5甲醇誘導表達后，活性電泳出現明顯活性條帶，重組酵母發酵液中mn - sod的活性約是對照菌株sod活性的2 . 6倍； cuzn - sod酶活力約是對照菌株sod活性的1 . 3倍。
  19. The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation

    Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇（ 3 : 5 v : v ）和kcn （ 5mmol l ）抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。
  20. Given the critical role of a - beta in the disease process, the proteases that produce this peptide are obvious targets for potential drugs that could inhibit their activity

    知道了a -在阿茲海默癥所扮演的關鍵角色，那麼會製造這個勝肽的蛋白酶就是明顯的目標，可以設法研發藥物，抑制這類蛋白酶的活性。
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