真接拷貝 的英文怎麼說
中文拼音 [zhēnjiēkǎobèi]
真接拷貝
英文
direct duplicating film- 真 : Ⅰ形容詞（真實） true; genuine; real Ⅱ副詞1 （的確; 實在） really; truly; indeed 2 （清楚確實） cl...
- 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
- 拷 : 動詞（打） flog; beat; torture
- 貝 : 名詞1 [動物學] （蛤螺等有殼軟體動物的統稱） cowry; cowrie; shellfis 2 （古代用貝殼做的貨幣） cowr...
- 拷貝 : [電影] copy; replica拷貝機 letterpress; 拷貝片 print; 拷貝紙 copy paper; copying paper
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In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。Multi - copies insertion transformants were screened on g418 plates. the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay. the n - carbamoylase activity of recombinant was 2. 26 and 2. 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169
將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。
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