真核體 的英文怎麼說
中文拼音 [zhēnhétǐ]
真核體
英文
eukaryote-
In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中,利用pcr克隆的方法擴增出bar基因全長,並在原核表達系統中表達,鑒定表達蛋白的活性,將能夠正確編碼ppt乙酰轉移酶的bar基因片段,經過適當的修飾構建入真核表達載體。The chromosomes of eukaryotes are nucleoproteins.
真核細胞的染色體是核蛋白。Cloning and sequecing analyzing of the hemagglutinin gene from influenza a h3n
基因真核表達載體的構建及序列分析We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0
我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ,然後利用脂質體轉染家蠶bmn細胞,瞬時表達hgh 。In this paper, through practical investigation, spot visit and scientific analysis, the problem which exits in the enterprise ' s inside encouraging system is studied, and by inducing, the following problems are pointed out : first lacking scientific result examination system, unfair distribution system ; second disjointed distribution and personal work - effect, lacking enterprise loudening power, lass of use - full personnel, lacking power of bringing forth the new ideas. in this paper, according the practical situation and combining with the theory of encouraging system, the author analyzed the problem in this enterprise and thought that the main reasons
本文作者結合所學的激勵相關理論知識,通過實際調查、現場訪問和科學分析的方法,研究該企業在激勵機制上存在的問題,經過認真分析、整理,認為該企業激勵機制上存在的主要問題是:首先在機制上缺乏科學的績效考核體系、分配製度不公;其次,在制度上分配與個人績效嚴重脫節;第三在管理方法上缺乏靈活性、企業缺乏凝聚力、人才流失,缺乏創新力。Mitochondria are present in all eukaryotic cells.
一切真核的細胞都有線粒體。Cloning of the complete coding sequence of mouse oxytocin receptor gene and its eukaryotic expression
小鼠催產素受體基因編碼區全長的克隆和真核表達On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection
本實驗以pegfp - n1質粒為骨架載體,用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體,雙切線性化后回收,使用回收的表達載體經原核顯微注射生產轉基因小鼠。( genbank submission no. ay190323 ) eukaryotic recombined expression vector, pcdna3
1和pegfp上,獲得真核重組表達載體pcdna3Monokaryons of this fungus will grow readily as saprophytes in plate or liquid culture.
這種真菌的單核體在平板或液體培養時能像腐生菌那樣容易生長。The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine
利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ,並對其進行原核和真核表達,是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎,具有極為重要理論和實踐意義。Cytochrome p450 functions as the monooxygenase. p450s are involved in physiologically important processes including steroid metabolism, drug deactivation, procarcinogen activation, fatty acid metabolism, xenobiotic detoxification and wildly distributed in animal, plant and low eukaryotic organism
細胞色素p450具有加單氧酶的作用,並能參與機體解毒、甾體激素的合成、脂肪酸代謝等重要的生理反應,並廣泛分佈於動物、植物和低等真核生物中。In the more advanced fungi, especially the basidiomycota, nuclei may fuse some considerable time after fusion of hyphae of different mating strains, forming a dikaryon
在較高等的真菌中,尤其在擔子菌門中,不同類型的菌絲融合后細胞核需要較長的時間進行融合,從而形成雙核體。This paper is a study on the expression of the protective gene of bont / a in the prokaryotic and eukaryotic. it indicates the possibility to produce the recombinant protein in quantities. it has also laid down a good foundation for the further research on vaccine or antibody of bont / a
本論文進行了人工合成的a型肉毒毒素hc段基因在原核和真核表達系統中的表達研究,使制備大量bont a保護型抗原的重組蛋白成為可能,為進一步進行a型肉毒毒素的疫苗或抗體研究奠定了一定的基礎。Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system
人骨形成蛋白3 ( hbmp - 3 )屬于tgf -超家族的一員,可以誘導間充質細胞分化為軟骨和骨,在胚胎時期骨骼發育和骨再生修復中起著重要的作用,而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用,因而hbmp - 3有廣闊的市場前景。它在人體內含量極微,盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達,但是由於原核表達系統缺乏翻譯后的加工修飾,真核表達系統存在成本高、產量低等特點,限制了其在臨床上的應用。We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm
Eif - 5a的hypusine修飾是其活性和功能發揮所必需的,我們通過pcr方法實現了hypusine位點的定點突變,並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。In eukaryotic cells, the enzymes and other components of the respiratory electron - transport chain are located in the inner membrane of the mtochondria
真核細胞中,電子傳遞鏈中的酶與其它成分位於線粒體內膜。( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
( 3 )翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ,在3端添加了內質網定位序列kdel ,構建了植物表達載體pbemt 。The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence, which interdicted the process of protein entering golgi body and cytoplasm, and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism. 2
真核分泌信號肽序列可以引導新合成的蛋白質進入內質網腔, kdel序列將進入內質網腔的蛋白質錨定在內質網內壁上,從而阻斷了蛋白質進入高爾基體和細胞質的過程,進而避免了外源蛋白質的異源糖基化修飾,延長了蛋白質在生物體內的半衰期。Studies on construction and immunity enhance of double expression plasmids of classical swine fever virus e
2基因真核雙表達載體的構建及其免疫增強作用的研究分享友人