Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破
碎細胞,離心,上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體,變性后,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據
毛山lel ,決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質
And then the fifth quarter is lost : all that raw stuff, hide, hair, horns
這下子連七零八
碎兒都沒有了,所有那些生料皮啦,
毛啦,角啦。
The creation tactics essence of this kind of plot ization is base on the premise that the subject matter and theme stipulate combined to distributing the true incident breaking to pieces, it makes their compact whole overcome memoir easy loosely organized, progress sluggish defect that appear s in creating, the vivid and abundant plot can strengthen personage s personality, artistic appeal of deepenning the theme, strengthenning the works
這種情節化的創造手法實質是在題材與主題規定的前提下對散
碎的真實事件進行整合,使之構成緊湊的整體,從而克服了回憶錄創作中容易出現的結構鬆散、進展遲滯的
毛病,而生動豐富的情節則可以強化人物性格、深化主題思想、增強作品的藝術感染力。
Take one recurrent issue : nail - clipping - sized fragments of plastic that end up knitted into the bolts of woolen fabric and must be laboriously spotted and removed by hand
舉一個時常發生事例:指甲大小的塑料
碎屑被織入
毛紡面料,就需要人工花大力氣找出並剔出。
You know one has always a world of little odd things to do after one has been away for any time.
你知道,一個人任何一次離開家再回來,總有許多零
碎的雞
毛蒜皮小事要做。
You have probably seen it in movies : when the moon is full, a man turns into a hairy, frightening creature and runs around the city, tearing people to pieces
你可能曾在電影中看到:月圓時分,一個男人變成
毛茸茸的駭人怪物在城市裡四處橫行,把人撕成
碎片。
The new housemaid is like a bull in a china shop. when she helps in the kitchen, she sometimes breaks a cup, and sometimes knocks dishes off the table
新來的女傭是個
毛手
毛腳的人。她在廚房幹活的時候,一會兒打
碎杯子,一會兒又把桌子上的碟子碰到地上。
Along with other data, these results indicate that tempel 1 is not a compacted snowball, as many had conjectured, but a loose, powdery fluff ball ? an agglomerate of primordial dust that came together at low speeds and gently clung like dust bunnies under a bed
綜合所有的數據來分析,譚普一號並非如大多數人所猜想,是顆結構緊實的大雪球,它比較像是一顆鬆散易
碎的
毛球,由遠古的塵屑在低速下緩慢沾黏在一起而形成,就像是床底下的那些小
毛球。
I packed dishes in towels to protect them
我用
毛巾把盤子包起來保護它們不會破
碎。
My pet cat clawed the only curtains to shreds at whim, and shed fur all over my house
我那頭寵物貓突然把全屋唯一的窗?撕成
碎塊,又把貓
毛弄得滿屋都是。
Fibroblast covered the wound and joined it. however, in the other groups, inflammatory reaction remains active, and there still were lots of degeneration and necrosis cells
而另兩組仍處于炎癥反應的活躍階段,可見大量的組織細胞變性壞死,細胞破碎,毛細血管內皮腫脹加重。