碼子 的英文怎麼說
中文拼音 [mǎzi]
碼子
英文
1. (數目符號) numeral 2. (籌碼) counter; chip-
( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed
( 2 )在翻譯水平上通過pcr擴增的方式在t - pa基因起始密碼子處添加了植物翻譯起始共有序列aaca ,構建了植物表達載體pbet 。It also carries a specific nucleotide sequence, the anticodon.
它還帶有特定的核苷酸序列即反密碼子。What is an anticodon, and what is its function
什麼是反密碼子,功能為Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量,我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列,目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上,構建ppic9k - fl表達質粒。Cebus symbol encoding sublayer physical layer conformance
Cebus符號編碼子層物理層一致性Cebus pl and rf symbol encoding sublayer physical layer conformance
Cebuspl和rf符號編碼子層物理層一致性Because of this coding relationship, triplet of bases is called a codon.
由於這種編碼關系,堿基的三聯體被稱為密碼子。Codon usage in genes coding for proteins with different folding types
不同折疊類型蛋白編碼基因的密碼子使用Correlation between the mutation of the dcc gene codon 201 and gastric cancer
基因201密碼子突變與胃癌相關性的研究Method for the design of mrna detecting genechip based on codon usage bias
一種基於密碼子使用偏性的檢測型基因晶元設計方法Chain termination codon
鏈終止密碼子Chain initiating codon
鏈起始密碼子Comparison and analysis of synonymous codon repeat sequences in different genomes
不同基因組中同義密碼子重復序列的分析與比較Among the 17 isolates, 8 isolates display tcg - ttg mutation in codon 531, 5 isolates have cac - tac or aac or cgc mutation in condon 526, 3 isolates have gac - gtc mutation in codon 516, and the last one has caa - aaa mutation in codon 513
其中8株為531位密碼子tcg ttg的突變, 5株為526位密碼子cac tac或aac或cgc的突變, 3株為516位密碼子gac gtc的突變,另一株發生了513位密碼子caa aaa的突變。The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form
本研究的內容包括:一、在前人對抗菌肽cmiv研究的基礎上,對n端和c端進行氨基酸保守變換,設計和合成了該基因,充分使用大腸桿菌偏愛的密碼子,並將該基因5端與硫氧還蛋白基因3端融合,通過ptxfus表達載體獲得較高可溶性表達(在15 sds - page膠上可見明顯的表達蛋白帶) 。Two spider silk protein genes ( avfl and avf2 ) screened from the library are characterized in a lot of repetitive motifs, a high guanosines or cytidines content, a strong preference for adenosine or thymidine in the third position of a codon and rich residues of glycines or alanines in the proteins translated
試驗結果,該文庫容量為4 . 9 10 ~ 6 。從文庫中篩選到avf1和avf2蛛絲蛋白新基因,具有典型重復序列多, g c含量高,密碼子第三個堿基偏愛使用a t及編碼蛋白中含有大量gly和ala殘基等特點。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -
S1基因:其全序列共1614bp (從起始密碼子atg到s前體蛋白裂解位點) ,編碼537個氨基酸,其氨基端有一編碼18個氨基酸的信號肽序列,第12 13位氨基酸殘基構成了信號肽的切割位點, 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。The start reading framae and stop codons, base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods
本研究在蛋白質編碼基因起始閱讀框和終止密碼子、蛋白質編碼區的堿基組中文摘要成、氨基酸及密碼子的利用等方面把少棘蜈蚣與另三種多足類進行了比較。Sv40 polya signal and two loxp sites was added consecutively after the termination codon taa, and then a gfp gene was inserted between the two loxp sites
在三個終止密碼子taa之後,連上sv40polya 。 sv40polya之後,又連上兩個loxp位點。在兩個loxp位點,插入標記基因gfp 。分享友人