種特異性抗體 的英文怎麼說
中文拼音 [zhǒngtèyìxìngkàngtǐ]
種特異性抗體
英文
species specific antibody- 種 : 種名詞(姓氏) a surname
- 特 : Ⅰ形容詞(特殊; 超出一般) particular; special; exceptional; unusual Ⅱ副詞1 (特別) especially; v...
- 異 : 形容詞1 (有分別; 不相同) different 2 (奇異; 特別) strange; unusual; extraordinary 3 (另外的;...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 特異性 : distinction
- 特異 : 1 (特別優異) exceptionally good; excellent; superfine2 (特殊) peculiar; distinctive特異功能 s...
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On the other hand, there is substanial evidences tha dcs mediate tolerane. the gm - csf - stbolated mouse bone mwderived mhc class ii + dc progendors tha are deficient in cell surface erpression of the costbolatory molecules b7 - l and b7 - 2 can induce alloanginspecific t ceil anergy in vbo. systendc adchstraion of these donor - derived dc progendors to recipients intravenously 7 days before transplantation prolonged the median graft survival tane from 9. 5 days to 22 days wbout additional twnosuppressions in a mouse cardinc twlanation model
1995年, lul等報道表面缺乏共刺激分子,特別是缺乏cd80 ( b7 - 1 )和cd86 ( b7 - 2 )的未成熟型dc能在體外誘導同種抗原特異性的t細胞無能,而對dc的適當處理能夠使未成熟型dc潛在的耐受原性得以放大和增強,使得dc在誘導免疫耐受中具有相當重要的作用及潛在的應用價值。Anti - keratin auto antibody ( ak auto ab ) is accepted generally as a significant component of natural auto antibody ( naa ) which is found in human as well as vertebrate, and is of characteristic of polyreactivity, low avidity, extensive connectivity and shorting of specificity to the species
天然自身抗體( naa )廣泛存在於人和脊椎動物體內,具有多反應性、親和力低、締和性廣及種屬特異性差的特點。From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting
利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法,但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性,利用特異性的豌豆鐵蛋白抗血清,就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測,從而更好地進行轉基因方面的研究。In group a, rabbits were immunized with isologous lens antigens ( il - ags ), + cfa while in group b rabbits were injected with pbs + cfa
方法:採用同種晶狀體抗原免疫家兔;觀察血清特異性抗體水平,晶狀體病理和裂隙燈下晶狀體形態學變化以及視覺電生理改變。Interleukin - 2 is a lymphokine synthesized and secreted primarily by t lymphocyte or t helper lymphocytes that have been activated by stimulation with certin antigens or mitogens. it is one of the important t cell growth factors. because of its key role in immune response, it is always a center in the study of cytokines. compared with human and mammalian, the study of chicken il - 2 is very slowly
白細胞介素- 2 ( interleukin - 2 , il - 2 )主要是在促有絲分裂素或特異性抗原刺激下,由t淋巴細胞或t淋巴細胞系產生的一種淋巴因子,是機體主要的t細胞生長因子之一。由於il - 2具有的重要免疫調節功能, il - 2一直是細胞因子研究中的熱點之一。2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody
選其中一株3 - h _ ( 11 )做雜交-雜交瘤細胞染色體計數,同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數:制備腹水型和上清型抗體,用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體,即紅細胞固相吸附法測雙特異中文摘要性抗體,紅細胞凝集試驗測單特異性抗人a型紅細胞抗體,間接elisa法測抗p24抗體;用腹水型抗體做耐熱性及耐凍融實驗。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選,在pap基因的3 』端融入了綠色熒光蛋白gfp )基因,然後將融合基因克隆在植物表達載體pbi121上,再進行遺傳轉化,得轉基因棉花種子5000餘粒,將種子播種長到于葉展開時,先在黑暗中用紫外燈照射,查找表現綠色熒光的幼苗,然後再用地高辛( dig )標記的pap基因特異性探針對這些棉花進行點雜交,最後發現有8株棉花表現陽性反應,說明pap基因的確己經轉到了棉花的基因組中,其棉花黃萎病的抗性鑒定正在進行之中。The who classification states that acute lymphocytic leukemia ( all ) should be classified by the pattern of reactivity of cell to a panel of lineage - associated antibodies and, where possible, genetic abnormalities. the expression of cd antigens on leukocytes is currently determined by flow cytimertry, which is expensive and labor - intensive, requiring 5 - 20ul quantities of fluores - cently labeled antibodies and allowing concurrent analysis for a limited number of cd antigens, usually three to four. from clinical point of view, an efficient method is required for analysis of a large number of samples in a single experiment
我們設計了一種新型的細胞晶元,其原理是充分利用玻片的硬度高,透明性好的特點,應用細胞膜表面不同的抗原物質,與包被在玻片上的不同抗體發生特異性結合,通過一次實驗可以將被測細胞懸液中不同膜表型的細胞分離、固定在同一張玻片的不同特定區域,保持了細胞的完整性及活性,又可通過簡單的操作一次將多細胞懸液進行免疫分群。In aqueous solution, interaction between crude venom and different bacteria including gram - positive bacteria, i. e. streptococcus mutans, streptococcus aureus, and gram - negative bacteria, i. e. e. colik12, was investigated in detail. molecule weights of multiple proteins were identified as 110kda, 47kda, 24kda, 23kda and 15kda by sds - page, respectively. it had little influence on extraction of snake venom proteins by the same cells of streptococcus mutans even used for 10 cycles
其次,在利用s . mutans分離細菌特異性抗體的基礎上,首次利用細菌篩選作用蛋白,將革蘭氏陽性菌( s . mutans和s . aureus )和革蘭氏陰性菌( e . colik12 )分別與蘄蛇蛇毒相互作用,發現有五種蛇毒蛋白能夠與細菌相互作用,其分子量分別為110kda 、 47kda 、 24kda 、 23kda和15kda ;同時表明s . mutans菌體能夠多次與蛇毒相互作用,並且對作用蛋白的結合沒有影響。When the antigen ( brief named ag ) invaded into the organism, and the body can produce a kind of material ( high molecular weight protein, named antibody, brief named ab ) that can distinguish this antigen and evacuate it, the antigen and antibody take place the immuno - reaction. using their highly sensitivity to response mass change and specificity, it can be fabricated a piezoelectric immunnosensors
當異種蛋白(稱為抗原, antigen ,簡稱ag )侵入生物體內時,體內能產生識別此類異物並將其排出的物質(大分子量蛋白質,稱抗體antibody ,簡稱ab ) ,抗原與抗體發生免疫反應,利用抗體(或抗原)對抗原(或抗體)的特異性識別功能和壓電晶體的高靈敏質量響應可製成壓電免疫傳感器。Scientists at newcastle university have developed a cancer fighting technology which uses ultra - violet light to activate antibodies which very specifically attack tumours
紐卡斯爾大學的科學家開發了一種對抗癌癥的新技術,這種技術是用紫外光激活能特異性攻擊腫瘤的抗體。Different kinds of salmonella antigens have been tried and an improved heat - extracted antigen was used as the diaphragm antigen. the optimum condition of dot - ppa - elisa was determined. the antibody positive standard of salmonella infection was established as 1 : 32, and the positive standard of protecting pigs from salmonella infection as 1 : 512
研究中對多種方法提取的沙門氏菌抗原進行了篩選,選取了特異性良好的抗原;確定了dot - ppa - elisa的最佳工作條件;初步確定了感染仔豬副傷寒的dot - elisa抗體陽性標準為1 : 32 ,豬只抵禦副傷寒感染的dot - elisa抗體保護標準為1 : 512 。It is well known that paf, a membrane lipid derivative molecule, is increased duing ischemia and hypoxia, which stimulates the further release of glutamate at presynaptic endings. ginkgolide b, one of the component of ginkgo biloba leaves, is a potent paf receptor antagonist
研究者們在篩選和尋找防治缺血缺氧性腦損傷的過程中,已經確實證明了天然銀杏葉的組分之一ginkgolideb是一種強有力的高特異性天然paf受體拮抗劑,可與位於突觸前膜的特異性paf受體結合。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體,分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒,並在大腸桿菌中誘導表達出相應的融合蛋白;用全長gstjqdrgz蛋白免疫兔,然後用gst ndrgz人和gstjqdrgze片段加強免疫,經免疫得到了較高效價的兔抗人ndrz多克隆抗血清,利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清,提高了ndrgz抗體的特異性;並對包涵體形式表達的6his ndrgz進行初步的分離純化。5 in vitro specific killing experiments showed that spleen cells induced by pi, p3 and p4, which contained higher proportion of cdg + cells and generated better ctl activity, could be introduced to in vivo experiment against hcv 6 we successfully setup a hcv structural gene in vitro cell - transfection system sp2 / 0 - hcv, through which also setup a murine model with subcutaneous transplanting tumor of hcv
將誘導的5種v特異性c l ,在體外進了丁特異性殺傷實驗,證一實,出n 、 p3和p4三條多肽誘導的脾糾泡含較高比例的cd 。糾1巾d ,叫產上較好的ctl殺傷活性,可以用作t細胞疫苗進fi體內抗hcv實驗。Results only 76 people with blood antibody titer > 1 : 5 had the cellular immune effect ( the particular plt was marc than 5 % ), 35 people without antibody and 4 people whose blood antibody titer was 1 : 5, nobody had the cellular immune effect
結果狂犬病疫苗接種后,抗體效價達到大於1 : 5的100人中, 76人同時產生細胞免疫力(即狂犬病毒特異性淋巴細胞轉化率大於5 % ) ;而抗體效價等於1 : 5的4人和未產生抗體的35人中,無人產生細胞免疫力。In the first part, we observed the changes of expressions of type i receptor of il - 1 in the rat and mouse brain after intraperitoneally administration of different kinds and doses antigens respectively. in the second part including two experiments we cloned rat il - 6r ' s genes by pcr, expressed them in e. coli dh5 a and cos - 7 cell, and produced il - 6r ' s polyclonal antibody which is proved having more high liter, affinity and specificity 1
第一部分採用不同種類和劑量的抗原刺激,分兩個實驗,觀察了大、小鼠腦內il - 1的i型受體表達的變化,探討了腦內參與免疫調控的核團和細胞類型;第二部分分兩個實驗,運用pcr技術克隆了大鼠il - 6r的基因並進行了原核和真核表達,制備了特異性高、親和力強和較高效價的抗il - 6r多克隆抗體,為進一步進行il - 6r的研究奠定了基礎。These phage display peptides may be the tnfa mimotopes. part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding - peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides. we used c7c tnfa mimotope phage clone lcs - 7 ( c - rrpaqsg - c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa
3 、以特異性噬菌體克隆為靶篩選肽庫的研究? ?一種肽庫篩選新方法的建立:為了深入研究細胞因於與配基(受體或抗體)相互作用的關鍵殘基及其空間構象,在上述研究基礎上,我們以tnfa表位模擬肽克隆為靶篩選噬菌體十二肽庫,找尋與之結合的tnfa結合肽噬菌體克隆;並以此拓寬了噬菌體肽庫的應用范圍。Currently commercially available specific antibodies as lab reagents are turning up fast and many labs make use of those commercial antibodies
目前國內外市場各種特異性抗體日益增多,許多實驗室都直接應用市售產品。分享友人