突變株 的英文怎麼說
中文拼音 [tūbiànzhū]
突變株
英文
mutant strain-
The acetaldehyde dehydrogenase ( acdh ) and alcohol dehydrogenase ( adh ) of the adhe mutants and parent strain were assayed with a result that the acdh activities of mutants were less than 10 % of the parent strain and the adh activities of mutants are about 10 % of the parent strain
6個adhe ~ -突變株的乙醛脫氫酶活性均降低為親株的10以下，乙醇脫氫酶活性降低為親株的10左右； 4個ldha ~ -突變株的乳酸脫氫酶活性均降低為親株的7以下。Through a series of mutagen tests, a mutant strain of monascus spp ( m 12 - 69 ) from ml2 was acquired, which can produce much higher monacolin k than m12, and almost same citrinin level as m12
以之為出發菌株，通過誘變篩選，獲得一株monacolink產率有明顯提高而桔黴素產率僅為0 . 13ng g （ elisa ）的突變株，編號為m12 - 69 。Infection of mutated mouse complement receptor type by epstein - barr virus
基因突變株的構建與毒力檢測Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins
將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。Two magnetosome deletion mutants were constructed by conjugative transposon mutagensis and the application of this genetic system. the two magnetosome deletion mutants were named as nm4 and nm21 respectively
並利用此體系，通過接合轉座誘變技術，獲得了2個磁小體缺失突變株： nm4 、 nm21 。Breeding of l - glutamic acid temperature - sensitive mutant
谷氨酸溫度敏感突變株的選育In most wild type e. coli strains containing heterologous pha synthase genes, the amount of pha accumulation was almost undetectable while in e. coli km32b the amount of pha accumulation were clearly visible
在多種野生型大腸桿菌中，盡管使用了各種不同的表達載體，也很難獲得可供檢測的pha ；但應用大腸桿菌的fqdb缺失突變株卻可以獲得較多量的pha 。Breeding of highly productive - linolenic acid mutant strain of mortierella ramanniance
亞麻酸高產突變株的選育Compared to 042bm, the noea deletion mutant 042bma - km showed different degree of increase in nodule number, weight of nodule nodule and plant top dry weight on cultivars of putong zihua, baoding, ningxia, baifa and aohan, but decrease on milu. however, this mutant has no significant change in ability to nodulate cultivars of huanghou and zahua. hence, noea is involved in cultivar - specific nodulation
與苜蓿中華根瘤菌042bm相比，敲除noea的突變株042bma - km在普通紫花、保定、寧夏、百發和傲漢苜蓿品種上的結瘤數、根瘤鮮重和植株地上部分的干重都有不同程度的增加，而在秘魯苜蓿品種上的結瘤數和植株地上部分的干重明顯下降，在皇后和美國雜花苜蓿品種上則沒有明顯的變化。Breeding of alkaline lipase overproducing strain by screening resistant mutant
抗性突變株篩選法選育堿性脂肪酶高產菌The main results obtained from this work are as follows : 1. the penicillium. sp ds9701, a strain of degrading phb, was mutagenized by uv treatment
本項工作主要是篩選能高效降解phb的突變株，並對其進行酶學性質的研究，主要結果如下： 1Whole cell c2d2 reduction by all four mutants comparing to wild type and ni / v mutant was also detected. the result showed that only single a - gln194 substitution did not perturb the stereospecificity of protonation of c2d2. the above comparing results indicate that in mofe protein ( 1 ) a a - gln190 site and its association with homocitrate are important for the transfer of electron / proton to femoco, while a - his194 site and the homocitrate are independent in h2 evolution
對四個突變株細胞的c _ 2d _ 2還原特性及還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例進行了測定並與野生型及nifv突變株相比較，結果表明只有- gln ~ （ 194 ）替換不影響c _ 2d _ 2還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例，即未改變固氮酶還原c _ 2h _ 2加氫的立體構型的專一性。4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。Isolation and characterization of nitrate reductase - deficient mutants of dunaliella salina
杜氏鹽藻硝酸鹽還原酶缺陷型突變株的篩選與鑒定This fact suggested that dndb and dndc were directly involved in the novel dna modification in wild type s. lividans
這兩個突變株均喪失了dnd表型，暗示這些基因與變鉛青鏈黴菌的異常修飾直接相關。The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase
尤其是在木聚糖酶譜帶檢測中發現，突變株發酵液中有三種類型的木聚糖酶，而出發菌株中只有兩種類型的木聚糖酶，並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現，突變株中第三種類型的木聚糖酶不僅表達量很大，活力也很高。281 double crossover mutants were examined by fermentation experiments and hplc analysis for production of polyketides. besides avermectin b1a and b2a, a new compound ( about l ~ 4ug / ml ) which showed a retention time and fragmentation patterns identical to those of the authentic sample of 22, 23 - dihydroavermectin bla by hplc and lc / ms analysis were detected in culture extracts of 27 isolates
在篩選的281個雙交換突變株中，有27株除了產生阿維菌素b1a和b2a ，還產生一個新組分，經hplc和lc ms分析，證實該組分為c - 22 ， 23雙氫阿維菌素b1a （即伊維菌素b1a ） 。The total amount of avermectins b were only about 0. 5 ~ 2 % of those produced by the parental strain olm73 - 12. this study suggests that t
在281個突變株中，還篩選到了2個突變株，其發酵產物幾乎全部為有效組分阿維菌素b1 ， b2組分僅佔5 。Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected
搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。The xylanase activity of the mutant kept stable after 10 generations. after orthogonal designing experiment, the optimum fermentation conditions of a. niger j 506 were obtained, which is as followed : concentration of the major carbon resource 4 %, ratio between bran and corncob 5 : 5, concentration of glucose 0. 1 %, concentration of ammonium oxalate as supplemental nitrogen resource 2. 0 %, the initial ph of liquid medium 5. 0, 100ml / 250ml flask
經過正交試驗設計，得出突變株a . nigerj506產木聚糖酶最佳的工藝條件為：主碳源濃度4 、麩皮與玉米芯的比例為5 ： 5 、輔加碳源葡萄糖的濃度是0 . 1 、輔加氮源草酸銨的濃度是2 . 0 ，培養基初始ph為5 . 0 ， 250ml三角瓶的裝液量為100ml 。分享友人