突變體蛋白 的英文怎麼說

中文拼音 [tūbiàntǐdànbái]
突變體蛋白 英文
mutant protein
  • 突 : Ⅰ動詞1 （猛沖） dash forward; shoot out 2 （高於周圍） protrude; bulgeⅡ副詞（突然） abruptly; sud...
  • 體 : 體構詞成分。
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr

    由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。
  2. The sds - page results showed the fusion protein was efficiently expressed in the soluble form. 3 ) the expressed fusion protein was purified and cleavaged by enterokinase to release the mutation i of cmiv and the mutation ii, whiches exhibited antibacterial activity to the ecoli. k12

    三、對以上表達的融合蛋白進行初步純化以及利用enterokinase切割融合蛋白，並進行抗菌活性檢測，結果表明所設計的cmiv突變體具有抗菌活性。
  3. Here we found g proteins also function in leaf, silique development and the yield of pollen microspore. we observed several traits or characters in the offsprings of gpal, agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type, whereas the lamina length, petiole length and rosette diameter is smaller than the wild type, the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type, and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines

    實驗中我們跟蹤觀察了多代異三聚體g蛋白a亞基超表達轉基因植株及a ， p亞基缺失突變體的表型特徵，發現突變體的葉片寬度大於對應的野生型，葉片長度，葉柄長度及蓮座直徑小於野生型，而超表達植株的上述某些特徵與突變體相反; gp時突變體的長角果長度，花梗柄部長度大於野生型，而超表達ga植株種英則略小於對照; gpal突變體長角果尖端未出現咭乙i突變體的特徵: gpal ，口gbl突變體花粉生成量大於野生型，而超表達ga植株的花粉生成量則略小於對照。
  4. They can convert plasminogen into plasmin and thus degrade fibrin. despite the widespread use of established thrombolytic agents such as streptokinase, t - pa and u - pa, all these agents suffer from a number of inadequacies including resistance to reperfusion, occurrence of coronary reocclusion and bleeding complications

    一些pa突變體及新型溶栓劑，如k2p tnk - pa和star等的臨床實驗結果表明它們在延長體內半壽期增強對血纖維蛋白選擇性和溶栓效率等方面有較大的改進。
  5. The quest continues for plasminogen activators with higher potency, specific thrombolytic activity, fibrin selectivity and longer half - life time. the recent progress in the protein engineering of plasminogen activators, including t - pa, u - pa, streptokinase, staphylokinase and other novel plasminogen activators, was presented in this article

    一些pa突變體及新型溶栓劑，如k2p tnk - pa和star等的臨床實驗結果表明它們在延長體內半壽期增強對血纖維蛋白選擇性和溶栓效率等方面有較大的改進。
  6. Construction, expression and cell growth inhibition of translocating peptide granzyme b fusion protein gene

    連環蛋白基因功能區截短突變體的構建及其功能
  7. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  8. Mass spectrometry of synthetic hw - ma and rgd - hw are in full agreement with those speculated theoretically, which proves the success of peptide synthesis and refold. on isolated mouse phrenic nerve - diaphragm preparations, hw - ma can block the neuromuscular transmission in 35 minutes or so ( l 10 - 5 g / ml ), its biological activity shows 73 % decrease comparing with biological activity of native hwtx - i. it proves t hat the protein engineering of synthetic chimera hwtx - i has gained success to some extent, although it did not achieve our expectations. thus it proved that hwtx - i can be using as natural scaffold for protein engineering. and also emphasized the importance of " local stereo circumstances " of activity site when the foreign activity site was transferred into a natural scaffold

    濃度為1 / 1059 / ml的hw一ma突變體能可逆阻斷小白鼠隔神經書高肌的接頭傳遞，阻斷時一間為35min左右，與天然hwtx一i比較，生物學活性下降3一4倍，說明合成的突變體改造獲得了一定的成功，盡管與我們預期的目標有一定的差距，從而證明hwtx一i可以作為蛋白質工程研究的天然分子骨架，同時也強調了往天然分子骨架中轉移外源活性位點時維持活性位點「局部立體環境」的重要性。
  9. Radioimmunoassay was used to test the immune activity of m - insulin fusion protein

    放免法測定人胰島素突變體融合蛋白的活性。
  10. Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway

    摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。
  11. Fourthly, we detected the mitogen activity of sed mutants and found that the mitogen activity of mutant sedn23a, sedn23a / h26r and sedp45a decreased significantly

    由於隨機突變的發生，我們還構建了sedn23aih26r雙突變體。並對突變體蛋白進行分析和純化，獲得了可用於后續功能實驗的sed突變體。
  12. Ie protein have multiple functions, such as conversion of the host cell into a metabolically activate states, activation of viral early genes in the early phase, repression of their own transcription. ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein

    經分析偽狂犬病病毒立即早期蛋白（ immediate - earlyprotein180 ， ie180 ）的蛋白質結構，發現ie180具有轉錄激活因子的結構特徵，能與類rna聚合酶結合， ie180基因缺失突變體對sv40及cmv啟動子調控作用的研究，是考察ie180能否作為轉錄激活因子應用的關鍵。
  13. After sds - page and densitometric scan analysis, the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 %. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %

    今士考個二目卜乙成功構建了ptxbi一hng及ptxbi一m一insulin原核表達克隆，並獲得了高效表達，經過純化得到純度人於80 %的融合蛋白，並對人胰島素突變體融合蛋白進行了初步活性測定。
  14. The ie 180 mutant combined with two special protein binding - sites and inhibited the promoter transcription. accidentally in the approach showed that the plasimd pcdna3 show as activator to sv40 and cmv early promoter. the result is acquired by instantaneous transinfect

    這說明不同的ie180突變體，對于sv40啟動于這類在轉錄起始位置一側只有一個「 5 』 atcgt 3 』 」特徵蛋白質結合序列的11類基因啟動于，可分別表現出抑制活性與激活性。
  15. We got some significant results regarding m - centrin by chemical methods. the titration of m - centrin by terbium ions was monitored by uv different spectrum and fluorescence spectrum

    利用紫外光譜和熒光光譜的方法探討了中心蛋白突變體和金屬離子及蜂毒素之間的相互作用。
  16. All of above main results suggested that ga was involved in pollen germination and tube grown by regulating cytosilic free calcium level, whereas gp was not, and extracellular cam might regulate pollen germination and tube growth through ga. the heterotrimeric g proteins had participated in several other development processes in arabidopsis

    從野生型和突變體花粉細胞中游離鈣離子水平對外源鈣調素的反應能力看， g蛋白參與了花粉萌發過程中外源鈣調素的跨膜信號轉導，且進一步通過其下游的鈣信使來調控。
  17. ( 3 ) we further demonstrated that the atcull component of scfco11 complexes was modified via axr1. mutations axr1 resulted in reduction of the modified atcull component of scfcol1 complex. ( 4 ) the mutant alleles of axri ( axr ] - 3 and axr ] - 12 ) showed moderate insensitivity - to ja - inhibitory root growth and mild reduction of ja - inducible gene expression of atvsp, thi2. i and pdfi. 2 compared with that of wild type

    以表達融合蛋白flag - coi1的axr1基因突變體axr1 - 3為材料，用- flag抗體進行免疫共沉澱分析發現， scf ~ （ coi1 ）復合體中修飾的atcul1蛋白的含量明顯降低，表明axr1基因突變影響對scf ~ （ coi1 ）復合體中修飾的atcul1蛋白的修飾。
  18. The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction. marker exchange method was used to study the relationship between the production of protease and the 3 orfs. a deletion mutant of xcc _ 4463 was constructed successfully

    Orf注釋表明，共中一個基因為胞外蛋白酶結構基因， 3個基因與合成調控有關， 5個與轉運分泌有關， 2個與lps合成有關，其餘3個orf的注釋功能與胞外蛋白酶的關系未見報道，為研究這3個orf與胞外蛋白酶產生的關系，採用同源雙交換orf缺失法進行了進一步驗證，成功地構建了xcc _ 4463缺失突變體，所得缺失突變體經檢測胞外蛋白酶減少，在寄主上致病性降低。
  19. The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied

    通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上，分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ，並分別導入無質粒突變株bmb171 ，篩選得到攜帶相應icps基因的重組菌株。
  20. 4. effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors. after resolution, the expression of cry1ac and cry 1c genes have no obvious change, but the expression of cry3a genes has great increase in the same condition

    解離反應對殺蟲晶體蛋白基因表達的影響成功地利用解離載體將crylac10 ， crylc和cry3a基因導入蘇雲金芽胞桿菌無晶體突變株， crylac和crylc基因解離前後的表達量和殺蟲毒力未見明顯變化， cry3a基因在相同條件下則表達量有所提高，至於為何只對基因cry3a有作用尚不清楚，國內外也未見有人作相關報道。
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