等電聚膠電泳 的英文怎麼說
中文拼音 [děngdiànjùjiāodiànyǒng]
等電聚膠電泳
英文
ife-
6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by sds - page
定量后，分別進行一維等電聚焦分離和二維聚丙烯酰胺凝膠電泳分離。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。Based on the lexicological biochemistry and ultrastructure, effects of heavy mental on 5. yangtsekiense had been studied in this paper by means of transmission electronmicroscope ( tem ) and polyacrylamide gel electrophoresis ( page )
本實驗採用了透射電子顯微鏡和聚丙烯酰胺凝膠電泳等方法，從超微結構和毒理生化兩個方面研究了重金屬鎘、鉛對長江華溪蟹主要組織器官的影響。A page gel, stained by the petiodic acid - schiff ' s method to reveal glycoproteins, further displayed that the bindng - protein was a glycoprotein belonging to lectin, which contained 17. 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method. peptide mapping was comparable to the reported protein in protein bank. the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase, aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b, glutamyl - trna reductase, histidyl - trna synthetase
連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 「 . skda ，由二個相同的亞基組成，亞基分子量為」 . ikda ，等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白，其中糖含量為17 . 44 % ，蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素By means of polyaerylamide gel electrophoresis, peroxidase isozyme, esterase isozyme and a - amylase isozyme of dioscorea zingiberensis, which were gathered from different growing environment conditions, were analyzed. with the results of 3 kinds of isozyme analysis and the analysis of 15 populations morphology and climate, dioscorea zingiberensis were divided into 5 ecotypes. the main results were shown as follows : 1
本研究以來自我國秦嶺山脈以南的甘肅、陜西、湖北、湖南、四川及雲南各省的盾葉薯蕷為試驗材料，通過對盾葉薯蕷的pox 、 est和a - amy3種酶的同工酶進行聚丙烯酰胺凝膠電泳分析，結合其形態性狀和物候期等生物學特徵，進行生態型的劃分，所得結論如下： 1In this thesis, we have done some research on the characterization of cu, zn - sod from hog blood by polyacrylamide gel electrophoresis, electric focusing electrophoresis and dealing with some different reagents
本文採用聚丙烯酰胺凝膠電泳，巰基已醇- sds -聚丙烯酰胺凝膠電泳，等電聚焦電泳以及各種凝膠層析的方法對豬血銅鋅超氧化物歧化酶進行了研究。By using the polyacrylamide gel electrophoresis, thirteen isozymes ( est, sod, gd, gpi, mdh, me, cat et al., ) from five population of r. philippinarum in the north of china were analised and compared
通過聚丙烯酰胺凝膠電泳對中國北方五個群體的菲律賓蛤仔的酯酶、超氧化物歧化酶、葡萄糖六磷酸脫氫酶、蘋果酸脫氫酶、磷酸葡萄糖異構酶、蘋果酸酶和過氧化氫酶等同工酶進行了分析比較。Pcr - based analysis method was carried out in the following study. dna amplification by pcr in cgg repeats were performed on 154 people as normal control ( 73 males and 81 females ) and 23 members from four x - linked mental retardation ( xlmr ) families. after electrophoresed on a 6 % - denaturing polyacrylamide gel ( acr : bis = 19 : l ), genotypes of the objects were determined by analyzing pcr products
本研究採用較以往更為簡便有效的pcr技術方法，對隨機抽取的154人的正常群體（男性73人、女性sl人）及來自四個智力低下家系的23名成員進行了fmr基因cgg重復序列的擴增，利用變性聚丙烯酸胺凝膠電泳技術對不同等位基因類型進行分離和統計。In order to understand the epidemiological behaviors of this pathogen, we studied strains e. coli 0157 isolated from patients and dung beetles in 1999 and 2000 in xuzhou city, jiangsu province, by methods of pcr, antimicrobial susceptibility, biochemical reaction and pfge ( pulse - field gel electrophoresis ) of chromosomal dna digested by restriction enzyme xbal
為了探索我國大腸桿菌o157 ： h7感染的流行病學特點，我們使用了聚合酶鏈反應、生化反應、抗生素敏感實驗、脈沖電場凝膠電泳等分子生物學方法，對我國1999 - 2000年徐州地區分離到的部分大腸桿菌o157 ： h7進行了分析。The peptides were extracted, and analyzed using an improved tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis ( tricine - sds - page ) method
方法通過針刺感染大腸桿菌誘導家蠅各蟲態產生抗菌肽，再經研磨、離心等步驟提取這些抗菌肽，用改進的聚丙烯酰胺凝膠電泳使其分離。This gives huanyu most advantages in paint industry in china. huanyu is engaged in the environment protection at all times, for example, in the area of reuse of waste polymerisates, in the area of making aqueous paint to restrict voc let out. especially in the reuse of polymerisates, by degradation, the macromolecule become oligomer which can be used as materials in paint resin manufacturing, both pet flack, grain, silk, bottle, block appearance and ps espccially in bubble form be widely used in huanyu. besides, huanyu has been using waste pe, pvc, pp to make aqueous paint package, the output reached 1 million size 5 gallons in 2000
公司主要產品包括：醇酸硝基聚酯聚氨酯氨基環氧陰極電泳氯化橡膠丙烯酸苯乙烯瀝青內外墻乳膠漆等12大系列， 1000多個花色品種，年生產塗料油漆2 . 6萬噸，產品的各項性能指標均達到或超過國家標準，並先後榮獲「中國綠色建材產品」 「中國環保產品質量信得過重點品牌」 「山東省名牌產品」 「山東省技術監督局免檢產品」 「濰坊市技術監督局免檢產品」等榮譽稱號。分享友人