篩選過的物質 的英文怎麼說

中文拼音 [shāixuǎnguòdewùzhí]
篩選過的物質 英文
screenings
  • 篩 : 名詞[書面語] （植物名） sedge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 過 : 過Ⅰ動詞[口語] （超越） go beyond the limit; undue; excessiveⅡ名詞（姓氏） a surname
  • 的 : 4次方是 The fourth power of 2 is direction
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 篩選 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; ...
  • 物質 : matter; substance; material
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  3. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. Metallophalocyanines ( mpcs ) are a kind of centrosymmetric planar organo - metallic molecules with an extensively delocalized two dimensional conjugated - electron system which show a relatively large third order optical nonlmearity, varying upon central metal atom substitution and other factors. other interesting properties of this molecule and many of its derivative products are their versatility, architectural flexibility and high environmental stability, which are very important requirements to implement photo - electronic applications

    因其骨架結構特徵和可通過選擇中心離子、軸向配體和在酞菁環上引入功能性取代基等方法進行分子篩選與組裝得到具有特殊的物理化學性質和光、電、催化等功能的材料，而引起化學家和材料學家的濃厚興趣。
  6. Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal

    本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究，並與生化位點標記分析法進行比較，旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法，為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。
  7. In order to study the influence factors of aoa of rose flowers, the effects of drying and extraction methods on the aoa of rose flowers were investigated. the results indicated that drying after high - temperature short - time pretreatment was rather effective to maintain their aoa ; the aoa of water extracts was stronger when the temperature was raised from 25 to 100 ; by using orthogonal test, the optimum extraction conditions of rose flowers were : solvent - 75 % ethanol ; ratio of material and solven - 1 : 10 ; extraction times - three times with 24 h at one time, at the room temperature. the extracts obtained by 75 % ethanol were fractionatedly extracted with petroleum, diethyl ether, ethyl acetate and n - butanol in turn, and the various fractions " aoa were analyzed

    為了探討玫瑰花抗氧化活性的影響因子，比較了不同乾燥方法、提取方法對其抗氧化活性的影響，發現：經短時高溫處理后再進行乾燥有利於較好地保持玫瑰花的抗氧化活性；以水作溶劑提取時， 25 100范圍內水提液的抗氧化活性隨著溫度的升高而增強；通過正交實驗篩選得到常溫下玫瑰花抗氧化活性物質的最佳提取方法為： 75乙醇為溶劑，液料比1 ： 10 ，提取3次，每次24h ； 75乙醇提取物依次用石油醚、乙醚、乙酸乙酯、正丁醇等有機溶劑進行兩相分部萃取，發現玫瑰花的抗氧化活性物質主要存在於乙酸乙酯部，說明玫瑰花抗氧化活性主要成分可能是單寧類、黃酮苷類和原花色素類化合物； 4
  8. The screening experiment of material combination was carried out for kudingcha drink, and the best material combination was chosen mainly according to sensory evaluation and the determination of chemical composition and physical properties. the results show that, when kudingcha gynostemma pentaphyllum makino wulong tea = 10 10 80 and the ratio of tea water is 1 120, it is more beneficial to the improvement of organoleptic quality and keeping - fresh during the process and storage of kudingcha drink

    為開發苦丁茶飲料提供依據，通過設置不同的原料組合，採用以感官審評為主，結合測定茶湯化學成分和物理性狀的方法，對加工苦丁茶飲料的原料配伍進行了篩選.結果表明，採用苦丁茶、絞股藍、烏龍茶的質量比10 10 80配製，以1 120的茶水比進行浸提，有利於改善苦丁茶的感官品質及其飲料加工、貯藏中的保鮮性能
  9. On the basis of the study of the theory and appraise method on land use in the small towns from home and abroad, this paper at first conducts a deep study on the development and role of the small towns, indicating that its development has sawn an uneven development phrase and becomes a carrier of the enterprises, a pool of surplus laborers, a hub of material exchanges between the rural and urban areas, a base of spiritual civilization, an important way to achieve urbanization. second, it conducts a study on the situation and features and the problems the land use, indicating that the efficiency of the land use is low, which has a direct influence on the development of agriculture and the role of the small towns. and the study of the demand of the land indicates the shortage of land is serious, and the small town must rationally use the land and increases its intensive role and the economical efficiency to meet the demand

    在分析國內外已有關于小城鎮土地利用的理論與評價方法的基礎上，首先對小城鎮在我國的發展、地位和作用進行了深入的分析，判明我國小城鎮發展經歷了一個曲折向上的發展階段，已成為鄉鎮企業的載體，農村剩餘勞動力的蓄水池，城鄉物資交流的樞紐，農村精神文明的基地，是我國城市化的重要途徑；其次，對小城鎮土地資源利用現狀和特徵進行了探討，並對發展小城鎮建設導致的土地利用問題進行了剖析，表明目前我國大多數小城鎮土地效益和規模效益低下，佔用耕地過多，直接影響農業的發展，影響小城鎮的地位和作用；通過小城鎮土地供需分析研究表明，我國土地短缺十分嚴峻，小城鎮土地需求缺口較大，小城鎮必須合理利用現有土地，增強集約功能和土地經濟效益，從而緩解需求壓力；最後，論文通過運用特爾菲法，描述統計分析法、多元統計分析（主成分分析）法和系統分析法中的層次分析法（ ahp ）等一系列方法，結合定性和定量兩方面，從土地質量、土地資源數量與結構、土地經濟效益、環境效益、社會效益等五個方面進行分析，篩選、建立了土地資源利用評價指標體系，在因子評價的基礎上，建立了土地利用綜合評價模型，並給出了評價過程和方法。
  10. At the same time i also did many primary experments on separator and these polymer membrane can be used in as soft packaging li - ion battery ’ s separator. used polyvinylidene fluoride ( pvdf ) as the basic material, added cotton fibre and starch in, cooked with 100 in boiling water afer 3 hours, the starch inflate, and then acquire porous polymer membrane. in the process of coating polyester film, copper foil and aluminum boil were used to as carrier,

    選用的基體材料為pvdf ，同時向其中添加棉纖維和澱粉，最後利用100沸水對隔膜進行3小時蒸煮處理使澱粉溶脹，從而達到造孔的目的。在塗布工藝的篩選中，通過對麥臘片、銅箔、鋁箔進行篩選，最後選定以銅箔為載體來進行塗布處理。當以銅箔為載體時，可以使隔膜正反兩面物理性質達到基本一致。
  11. Green fluorescent protein is widely applied in researches of modern life science, such as gene product moved - process in vivo, protein localization, drug screening and preliminary selection of transgenic individual as a molecular marker

    綠色熒光蛋白這種獨特的生物學特性，使其作為報道基因在現代生命科學研究領域中，如細胞內基因產物的動態過程，蛋白質在細胞內的定位，藥物的篩選，以及轉基因個體的初步鑒定等等有著廣泛的應用。
  12. Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway

    摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。
  13. Allergic reactions to animals are caused by a protein that is excreted in saliva, skin glands and urine. the special felines were selectively bred by reducing this trigger protein

    對動物的過敏反應源於一種存在於唾液，皮腺和尿液中的蛋白質。這種特別的貓咪是經過篩選培育出來的，以減少這種引發過敏反應的蛋白質。
  14. Biodiversity exists among am fungi and is influenced by numerous factors including soil properties and plant species. if am fungi are to be used in sustainable agricultural systems it is necessary to study native am fungi in the target areas and then select efficient isolates that can be applied as inocula in the field to improve crop growth. the objectives of this study were to investigate the germplasm of am fungi, to understand the distribution pattern of am fungi in different ecological conditions such as area, soil factor and host plant, to select isolates effective in nutrient acquisition by the host plant sweet potato, to test their effectiveness under field conditions, and to monitor amf after their introduction into the field

    本研究通過調查我國北方部分地區的am真菌資源，研究了am真菌的種群組成及其在空間、土壤利用方式和宿主植物類型等不同環境條件和空間尺度上的分佈規律；在此基礎上，根據它們對甘薯的生長、吸磷效應篩選出高效菌株，在大田條件下研究了am真菌菌絲的分佈特性、代謝活性及其對甘薯產量和品質的影響；並通過分子探針跟蹤調查了引入am真菌在共生體中的發育和表達，以期為菌根真菌的生產應用提供技術支持。
  15. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  16. In this construct, hf2 dna should be expressed under the control of the camv 35s promoter. the construct was transformed to petunia hybrida of the light pink via agrobacterium tumefaciens eh a105 using the leaf disc method. in the end, three transgenetic plants were obtained by screening with the phosphinothricin resistance and pcr amplification

    通過三親交配將重組質粒pc3301 - hf2導入根癌農桿菌eha105 ，抗性篩選、 pcr檢測及dna點雜交表明轉化后的農桿菌帶有完整目的基因片段，能夠用於轉化植物。
  17. In conclusion, the results of checking of physiochemistry items, uv spectrum data, chromatographic fingerprints test and safty evaluation indicated that gpif avivity evaluation samples were eligible. through the comparison of methods for evaluating immunobiologic activity and the choosing of optimum methods to evaluating immunobiologic activity, we created assay methods evaluating immunobiologic activity for quality standard of gpif. the research of dose - effect of gpif can also supply theoretical materials for pharmacology. that the experiments on the factors which affect the immunobiologic activity of gpif and the comparison with similar drugs demonstrated that gpif was a kind of cellar immunoregulating drug with stable and high immunobiologic activity

    綜上所述，通過對gpif免疫生物活性評價實驗樣品材料的準備，顯示由該工藝制備的實驗樣品材料理化性質穩定，活性成分含量充足，安全可靠，可以用來作為gpif免疫生物活性的評價;通過對gpif免疫生物活性評價方法的比較篩選及評價方法的優化，為gpif質量標準建立了較優免疫生物活性評價手段;同時gpif免疫生物活性劑量效應的發現，為該產品的免疫生物活性深入研究奠定了基礎，也為gpif藥效學研究提供了理論資料;對gpif免疫生物活性影響因素的探討及與同類藥品免疫生物活性的對比，證實gpif是一種免疫生物活性穩定、且具有高免疫生物活性的細胞免疫增強劑;同時，本研究也為同類產品免疫生物活性評價提供了方法學參考。
  18. Through rainfall simulating under laboratory, and making slice of sampling, the project researched in the soil crust development, and studied the dynamic rule of erosion factors which were raining and soil erosion factors during soil crust development. in the same time, the research was to find the critical condition of all factors in the process of soil crusting, and to filter the critical factor that could affect soil surface crust

    本文採用人工模擬降雨方法，通過采樣製作土壤切片，觀察分析表土結皮在不同條件下發育的微形態特徵，研究降雨、土壤等侵蝕產沙因子在表土結皮發育過程中的動態響應規律，界定表土結皮發育過程中各因子的臨界條件，篩選有關土壤顆粒組成、結構特徵等物理化學性質對表土結皮形成影響的關鍵因子。
  19. Few researchers doubt that plants and other sources of natural products are superior sources of molecular diversity and novel molecular chemotypes, particularly in the areas where good synthetic leads do not exist and the notion that evolution has been selecting and perfecting diverse bioactive molecules for much longer than any pharmaceutical company cannot be ignored

    一些研究者質疑植物或其他自然物質是否會是更好的藥物來源，這觀念在那些具有優秀合成藥物技術的地區更為明顯，但在長期的演化過程中，大自然確實篩選出完美且多樣化的活性物質，這一點是任何藥廠皆無法忽視的。
  20. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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