粒細胞毒素 的英文怎麼說
中文拼音 [lìxìbāodúsù]
粒細胞毒素
英文
gct- 粒 : Ⅰ名 (小圓珠形或小碎塊形物) small particles; grain; granule; pellet Ⅱ量詞(用於粒狀物)
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 素 : Ⅰ形容詞1 (本色; 白色) white 2 (顏色單純) plain; simple; quiet 3 (本來的; 原有的) native Ⅱ名...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
- 毒素 : 1. [生物學] (有機體產生的有毒物質) toxin 2. (言論或著作中有腐蝕作用的成分) poison
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First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Alcohol, the most common cause, is a hepatotoxin that interferes with mitochondrial and microsomal function in hepatocytes, leading to an accumulation of lipid
乙醇是最常見的病因,作為肝毒素能阻礙肝細胞內的線粒體和微粒體的功能,導致脂質沉集。Indication : treatment of acute or chronic viral hepatitis ( type b, c ), pointed condyloma, hairy cell leukemia, chronic granulocyte leukemia, lymphoma, kaposi ' s sarcoma, malignant melanoma concerned with aids
:用於急慢性病毒性肝炎(乙型、丙型等) 、尖銳濕疣、毛細胞白血病、慢性粒細胞白血病、淋巴瘤、愛滋病相關性卡波濟氏肉瘤、惡性黑色素瘤等疾病的治療。Indication : treatment of acute or chronic viral hepatitis ( type b, c ), herpes zoster, pointed condyloma, viral meningitis ( influenza virus, mumps virus, adenovirus ), hairy cell leukemia, chronic granulocyte leukemia, renal cell carcinoma, kaposi ' s sarcoma, lymphoma, malignant melanoma concerned with aids and other virus and tumor diseases
:用於急慢性病毒性肝炎(乙肝、丙肝等) 、帶狀皰疹、尖銳濕疣、病毒性腦膜炎(流感病毒、腮腺炎病毒、腺病毒) 、毛細胞白血病、慢性粒細胞白血病、腎細胞癌、愛滋病相關性卡波濟氏肉瘤、淋巴瘤、惡性黑色素瘤等病毒性及腫瘤性疾病。The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction
目的基因經雙酶切后連接載體ppic9k ,然後導入大腸桿菌dh5中,在含氨卞青霉素( amp )的lb板上用pcr反應篩選出陽性菌落,雙酶切結果表明目的基因已插入載體中,且方向正確,測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。The effects of sfamnpv on apoptosis - related protein bcl - 2 and cyto c were investigated by western blotting. sfamnpv could down - regulate the level of bcl - 2 expression after induction for 8h. also, cyto c was released from mitochondrial to cytosolic after induction for 4h
雜交可看到sl - 1細胞在接種病毒的4h后線粒體中的細胞色素c被釋放到了細胞質中,這與很多在哺乳動物細胞中所作的結果是相同的。Interaction of beta - carotene on rat pneumocytes exposed to cigarette smoking particle extracts
胡蘿卜素與香煙煙氣顆粒物對體外大鼠肺細胞毒性的交互作用Protective effects of genistein on ovarian granulosa cell toxicity in normal female rats induced by cisplatin
順鉑對正常大鼠卵巢顆粒細胞的毒性和金雀黃素的保護作用It widely applies to particle separation of samples like biologic cell, protein and hormone virus in organizations of r & d and production in fields of biology, genetics, pharmacology and etc
該系列離心機廣泛應用於生物學、遺傳學、醫藥學等科研和生產單位對生物細胞、蛋白質、激素病毒等樣品量要求少的粒子進行分離。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。分享友人