糖基化蛋白 的英文怎麼說
中文拼音 [tángjīhuàdànbái]
糖基化蛋白
英文
glycosylated protein-
The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點，包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等，拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能，而磷酸化位點是arc1參與信號傳導過程所必需的。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較，二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區，都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點，所不同的是： （ ）在結構功能域上， 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區； （ 2 ）在功能位點上， 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區，但沒有n糖基化位點； （ 3 ）擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性（ eThe recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence, which interdicted the process of protein entering golgi body and cytoplasm, and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism. 2
真核分泌信號肽序列可以引導新合成的蛋白質進入內質網腔， kdel序列將進入內質網腔的蛋白質錨定在內質網內壁上，從而阻斷了蛋白質進入高爾基體和細胞質的過程，進而避免了外源蛋白質的異源糖基化修飾，延長了蛋白質在生物體內的半衰期。The major products includes 1 ) food additives : citric acid & its derivatives, l - lactic acid & its derivatives, msg, starch sugar etc ; 2 ) feed additives : lysine & its salts, corn gluten powder etc ; 3 ) bio - energy : fuel ethanol, bio - diesel etc ; 4 ) biochemical products range : bio - ethylene & its derivatives, poly lactic acid ( pla ) bio - degradable plastics, poly lactic acid polymer fiber fabrics etc
主要產品有檸檬酸及其鹽類、 l -乳酸及其衍生物、味精、澱粉糖等食品添加劑；賴氨酸及其鹽類、玉米蛋白粉、氨基酸蛋白粉等飼料添加劑；燃料乙醇、生物柴油等生物能源產品；生物乙烯及其衍生物、聚乳酸生物可降解塑料、聚乳酸聚酯纖維布料、無毒綠色溶劑、無毒增塑劑等生物化工系列產品。Expression system renders products that are free of post - translational modifications, it is likely that the binding of crylab toxin to bt - r3 protein via protein - protein interaction and does not require glycosylation
且該受體蛋白與cry1ab的結合部位位於bt - r3受體蛋白的胞外結構域。 bt毒蛋白與鈣粘蛋白受體的結合與受體是否糖基化無關。It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
跨膜區域（ tmd ）將它分為胞內和胞外兩個部分，它的胞外有13個潛在的糖基化位點， 20個蛋白激酶c的磷酸化位點， 28個酪蛋白激酶的磷酸化位點， 4個酪氨酸酶的磷酸化位點， 15個豆蔻（十四烷基）酰化位點；它的胞內有1個整合蛋白（ integrin ）識別位點。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。Effect of advanced glycosylation end products on the expression of connective tissue growth factor and fibronectin in cultured human mesangial cells
糖基化終產物對人腎小球系膜細胞結締組織生長因子及纖維連接蛋白基因表達的影響Patients enrolled in the trial ranged from 12 to 80 with baseline hemoglobin a1c greater than 7. 5 % and were already using an insulin pump to treat their diabetes
參與試驗的病人年齡是從12歲到80歲，他們的糖化蛋白a1c的基線都大於7 . 5 % ，已經用胰島素泵進行治療。Mammalian cells are among the best systems for biopharmaceutical production due to their excellence in post - translational modifications. the products they produced are much more similar to their natural forms than those produced by prokaryotic, yeast or insect cells
哺乳動物細胞表達系統具有準確的轉錄后修飾功能，表達的糖基化蛋白藥物在分子結構、理化性質和生物學功能方面最接近天然蛋白分子，是目前重組糖蛋白藥物生產的首選體系。Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids
Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵，包括36個氨基酸組成的疏水信號膚，一個cub （ clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl ）結構域和一個胰酶樣絲氨酸蛋白酶結構域（ t汀psin一likeserineproteasedomain ）和幾個保守的糖基化位點等，沒有發現有跨膜區的存在。The gene was 1668bp in length, encoding the f protein composed of 553 amino acids. sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids, six potential glycosylation sites and thirteen cysteines. a sequence region of basic amino acids, rrqrrf, was found at the f, - f2 cleavage site, indicating that f4ge9 was a typical virulent strain
序列分析和二級結構預測表明， f _ （ 48 ） e _ （ 9 ）株f蛋白含有3個分別由25個氨基酸組成的疏水區，存在6個潛在的糖基化位點， 13個半胱氨酸殘基，裂解位點區域的氨基酸序列為rrqrrf ，說明f _ （ 48 ） e _ 9株是一株典型的強毒株。Sequence analysis showed that the full length of this cdna which encodes 364 amino acids is 1398bp. it has 71 % and 69 % identities to lycopersicon esculentum and pisum sativum respectively in amino acid level. this gene is a membrane protein which has one signal peptide, seven transmembrane helices, three n - glycosylation sites and one o - glycosylation site
序列分析表明，該基因的cdna全長為1398bp ，開放閱讀框為1095bp ，編碼一個364個氨基酸的多肽，與番茄和豌豆中的該家族基因分別具有71和69的氨基酸同源性，是一種膜蛋白，具有1個信號肽序列， 7個跨膜螺旋， 3個n ?糖基化位點和1個o ?糖基化位點，分子量大約為39 . 174kd ，等電點為8 . 07 。Protein glycosylation inhibitors
蛋白質糖基化抑制劑Protein glycosylation, overview
蛋白質糖基化Proteoglycans represent a special class of glycoproteins that are heavily glycosylated
是一類高度糖基化的糖蛋白的代表。Inhibitory effects of total flavones of buckwheat flower on the non - enzymatic glycation of proteins in vivo and in vitro
蕎麥花總黃酮對體內外蛋白質非酶糖基化的抑制作用The results of sds - page and western blot showed that the culture supernatant of transforments contained the correct glycosylated e2 protein and the recombinants could still secretedly express the specific proteins after culturing for 8 generations
經甲醇誘導表達后， sds - page和westernblot結果證明了p . pastoris培養上清液中含有正確糖基化的e2蛋白，表達量約250mg / l 。Clinical significance of serum concentrations of advanced oxidation protein products and advanced giycation end products in patients with acute coronary syndrome
急性冠脈綜合征患者血清晚期氧化蛋白產物和糖基化終末產物的測定意義分享友人