糖基化酶 的英文怎麼說
中文拼音 [tángjīhuà]
糖基化酶
英文
dna glycosylases: mechanismsdna-
Based on recent molecular phylogenetic analyses using nucleotide sequences of the encoding the large subunit of ribulose 1, 5 - bisphyosphate carboxylase / oxygenase ( rbcl ), hypodematium should be not included in the athyriaceae, it has closely related to dryopteridaceae. on the other hand, athyriaceae, thelypteridaceae, blechnaceae, onocleaceae and woodisaceae form a large clade, so it may explain that tryon & tryon ( 1982 ) and kramer & kato ( 1990 ) putting it forward as dryopteriaceae s. 1
運用cpdna基因組編碼的磷酸核酮糖羧化酶大亞基( rbcl )的基因序列測定而構建的系統樹,顯示蹄蓋蕨科、金星蕨科、烏毛蕨科以及其他科構成一條與鱗毛蕨科平行的分支,因此可以說明kramer & kato ( 1990 )把蹄蓋蕨科放入廣義的鱗毛蕨科是不合理的。The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明, pcr擴增產物中包含有完整的phya基因,該基因全長1506bp ,其中包含一段長102bp的內含子,該內含子具有真菌植酸酶基因內含子的特徵保守序列: donor序列? gtatgc , lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸,理論分子量為51 . 37kda ,其上有13個潛在的n -糖基化位點, n端19個氨基酸為信號肽序列,植酸酶活性位點序列( crvtfaqvlsrhgaryptdskgk )位於氨基酸序列的+ 71 + 93 。The polysaccharide moiety of glucose oxidase contains n - acetylglucosamine and mannose
葡萄糖氧化酶的多醣構造中,含有n -乙醯胺基葡萄糖及甘露糖。D ) the polysaccharide moiety of glucose oxidase contains n - acetylglucosamine and mannose
葡萄糖氧化酶的多醣構造中,含有n -乙醯胺基葡萄糖及甘露糖。The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點,包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等,拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能,而磷酸化位點是arc1參與信號傳導過程所必需的。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eThe obtained biosensor exhibits high sensitivity, excellent reproductivity and good stability with substantially improved performance. part two describes the manufacture and characterization of glucose oxidase - silver sol - polyvinyl butyral modified platinum electrodes with tris ( 2, 2 ' - bipyridyl ) cobalt ( iii ) perchlorate as an electron transfer mediator in the glucose solution
用納米ag溶膠固定god ,採用聚乙烯醇縮丁醛為輔助固酶膜基質修飾鉑絲電極,並以葡萄糖溶液中的co恤pyh ( cio4 ) 3為電子媒介體組成葡萄糖氧化酶生物傳感器。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。Sodium ferulate inhibits nonenzymatic glycation of myocardial tissue in diabetic rats
阿魏酸鈉抑製糖尿病大鼠心肌非酶糖基化的形成From the results obtained in the determination of km for each glycoform, the following conclusion could be made. which of the following statements is / are correct
根據測定改變糖基型式的葡萄糖氧化酶之km值的結果,下列何者(哪些)是正確的敘述?A new enzymatic electrode for glucose determination was developed by codepositing glucose oxidase into the polymer from electrochemical oxidation of dopamine ( da ) on a pt - modified 8b pencil lead electrode, followed by electrochemically depositing an outer poly ( o - aminophenol ) film, which exhibited very good performance, such as fast current response, high sensitivity, broad linear range, excellent stability and good anti - interferent ability for ascorbic acid and uric acid
摘要市售8b鉛筆芯經鍍鉑處理后,將葡萄糖氧化酶共沉積到新型多巴胺電氧化聚合膜中,最外層再修飾聚鄰氨基酚薄膜,研製出新型葡萄糖氧化酶修飾電極用於葡萄糖檢測。Determination of glucose by the novel glucose oxidase electrode based on prussian blue in juice beverages
基於普魯士藍的葡萄糖氧化酶電極用於果汁飲料中葡萄糖測定It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
跨膜區域( tmd )將它分為胞內和胞外兩個部分,它的胞外有13個潛在的糖基化位點, 20個蛋白激酶c的磷酸化位點, 28個酪蛋白激酶的磷酸化位點, 4個酪氨酸酶的磷酸化位點, 15個豆蔻(十四烷基)酰化位點;它的胞內有1個整合蛋白( integrin )識別位點。( 5 ). glucose oxidase glass micro - pearls, the biology sensitive materials was prepared based on oxygen sensitive materials made by thermo - polymerization method, and could be utilized to detected the concentration of glucose in solution
( 5 )氧敏感材料在光纖葡萄糖傳感器中的應用:採用熱聚法制備氧敏感材料並在此基礎上制備了生物敏感材料? ?葡萄糖氧化酶玻璃微珠。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 )為材料分離克隆到一個細胞色素p450基因,命名為bccyp86mf5 , cdna全長1854bp ,含1575bp的完整開放閱讀框,編碼524個氨基酸,其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ;堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ;二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域,半胱氨酸亞鐵血紅素配體信號區等, -螺旋和-折疊分別佔47 . 7 、 45 . 0 ;與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ,與擬南芥cyp86c4的達到85 . 9 。In this paper, the conditions of the preparation of polypyrrole / glucose oxidase electrode and the characteristic of the polypyrrole gas sensor was discussed
論文對基於絲網印刷電極的聚吡咯氣敏傳感器的氣敏特性和聚吡咯在固定葡萄糖氧化酶制備葡萄糖氧化酶電極中做了初步的研究和探討。Objective : polypeptide : n - acetylgalactosaminyl transferase is the initiation enzyme catalyzing the linkage of o - glucan chain. recent study shows that o - glucosylation is closely related to molecular recognition, tumor formation, development and metastasis, as well as embryonic development. due to the initial study on function of o - glucosylation in china, this thesis aims to obtain stably expressed pp - galnac - t2 gene clones for further study
目的多肽: n乙酰氨基半乳糖轉移酶是合成o糖鏈的起始酶,而目前的研究認為, o -糖基化與分子及細胞識別、腫瘤的發生發展和轉移以及胚胎發育等功能密切相關。Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids
Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵,包括36個氨基酸組成的疏水信號膚,一個cub ( clr / cls , anembryonicseaurehinproteinuegf , andbonemorphogeneticproteinl )結構域和一個胰酶樣絲氨酸蛋白酶結構域( t汀psin一likeserineproteasedomain )和幾個保守的糖基化位點等,沒有發現有跨膜區的存在。Inhibitory effects of total flavones of buckwheat flower on the non - enzymatic glycation of proteins in vivo and in vitro
蕎麥花總黃酮對體內外蛋白質非酶糖基化的抑制作用Isolation and characterization of defense response genes involved in neck blast resistance of rice
水稻蔗糖轉化酶基因的克隆及其功能的初步探討分享友人