糖測序 的英文怎麼說

中文拼音 [tángcèxù]
糖測序 英文
carbohydrate sequencing
  • 糖 : Ⅰ名詞1 [化學] （碳水化合物） sugar 2 （食糖的統稱） sugar 3 （糖果） sweets; candy; sweety Ⅱ形容...
  • 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
  1. Based on recent molecular phylogenetic analyses using nucleotide sequences of the encoding the large subunit of ribulose 1, 5 - bisphyosphate carboxylase / oxygenase ( rbcl ), hypodematium should be not included in the athyriaceae, it has closely related to dryopteridaceae. on the other hand, athyriaceae, thelypteridaceae, blechnaceae, onocleaceae and woodisaceae form a large clade, so it may explain that tryon & tryon ( 1982 ) and kramer & kato ( 1990 ) putting it forward as dryopteriaceae s. 1

    運用cpdna基因組編碼的磷酸核酮糖羧化酶大亞基（ rbcl ）的基因序列測定而構建的系統樹，顯示蹄蓋蕨科、金星蕨科、烏毛蕨科以及其他科構成一條與鱗毛蕨科平行的分支，因此可以說明kramer & kato （ 1990 ）把蹄蓋蕨科放入廣義的鱗毛蕨科是不合理的。
  2. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  3. G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

    本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。
  4. In this paper, 45 e. coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e. coli by animal test. type 1 pili of 45 strains isolated was detected by msha. the pila gene of 45 avian pathogenic e. coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank. results showed that pcr was more sensitive, faster and more characteristic than msha to detect type 1 pili

    本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株，採用d -甘露糖敏感血凝試驗（ msha ）檢測1型菌毛，根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。
  5. The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank. concentration of tp was analyzed after amplification invitro by rt - pcr, purified by low melt agarose and labeled by digoxigenin

    根據genbankth - 98株序列設計第2對引物，應用rt - pcr方法體外擴增該片段（命名為tp ） ，瓊脂糖凝膠純化后測定其濃度和純度，進行非放射性地高辛標記。
  6. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  7. A aada2 cassette, encoding aminoglycoside adenylyltransferase, was found in 2 isolates of shigella sonnei, and a two cassettes array of dfra1, encoding dihydrofolate reductase, and aadala in one isolate of shigella sonnei, and another two cassettes array of dfra1 7 and aada5 in two isolates of shigella sonnei and shigella flexneri y - variant each, and a dfrv cassette in one isolate of shigella flexneri 6, respectively

    對其可變區測序分析，其中2株宋內志賀菌i類整合子均攜帶1個基因盒，為氨基糖昔腺營酞基轉移酶（ aminoglycosideadenylyltransferase ） az基因（ aa朔2 ） ，編碼對鏈黴素的抗性。
  8. Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively

    Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
  9. Plant expression vector pbil21 - cspds was constructed in which the expression of cspds gene was under the control of camv35s promoter and t - nos terminator via genetic engineering

    對番紅花屬番紅花的核糖體55一rr膚基因序列進行基因克隆、測序，獲得了的55一rrna基因的序列特徵。
  10. The gene was 1668bp in length, encoding the f protein composed of 553 amino acids. sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids, six potential glycosylation sites and thirteen cysteines. a sequence region of basic amino acids, rrqrrf, was found at the f, - f2 cleavage site, indicating that f4ge9 was a typical virulent strain

    序列分析和二級結構預測表明， f _ （ 48 ） e _ （ 9 ）株f蛋白含有3個分別由25個氨基酸組成的疏水區，存在6個潛在的糖基化位點， 13個半胱氨酸殘基，裂解位點區域的氨基酸序列為rrqrrf ，說明f _ （ 48 ） e _ 9株是一株典型的強毒株。
  11. Four colonies of transformed e. coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained. the gene fragment in these isolates was identified by the methods of plasmid processing. dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b. subtilis from 2599451 to 2812870 was 85 %, and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b. subtilis ) in genebank

    測序並序列比較結果表明該基因片段同已發表的枯草芽孢桿菌幾丁質酶和內切葡聚糖酶編碼幕因的克隆及重組芽抱桿菌的構建glyb一apre之間的同源性是最高的，為35 % ;同bacz 』了了ussp . bp23ce1b 、 b . p朋刀us內切葡聚糖酶和b . pol理vxap一1 ， 4一內切葡聚糖酶的編碼基因的同源性只有27 % 。
  12. Its and 5. 8s rrna genes of 4 species in the cardamine were sequenced. sequences of all species are complete in the useful segments and the results of the two - way sequencing are complementary sequences, which indicates that the evolution of its repeating units of those species had synchronized with a high degree

    ） 4種植物的核糖體dna中的內轉錄間隔區（ its ）序列及5 . 85 : rna基因全序列，在有效的區段內，序列數據完整無缺，且正、反向測序結果完全互補，這表明所用材料的its重復單位同步進化程度很高。
  13. The activity of the invertase of three high - sugar saccharomyces cerevisiae strains were investigated and their amino acid sequence were determined by the use of modern biotech including enzymatic activity determination, specific pcr, the cloning sequencing and bio - information analysis etc

    摘要採用現代生物技術中的酶活測定、特異pcr 、克隆測序和生物信息分析等對比研究3株高糖酵母蔗糖轉換酶活性及其氨基酸序列。
  14. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
  15. The nucleic acids sequence and amino acids sequence of xynbi were homologously analysed with the sequences coding for xylanases published in genebank. the result indicated that xynb ] was the highest similarity to the gene xyli from sm ptomyces sp. strain s38, up to 86 %

    將所測出的基因xynb1的成熟蛋白部分核苷酸序列與genebank上的木聚糖酶基因序列進行同源比較，最高同源性為86 ，氨基酸序列最高同源性也為86 ，並且與之同源性最高的基因所表達的酶酶學性質與xynb酶學性質有較大差異。
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