紅黴素鏈黴菌 的英文怎麼說
中文拼音 [hóngméisùliànméijūn]
紅黴素鏈黴菌
英文
streptomyces erythraeus- 紅 : 紅Ⅰ形容詞1 (像鮮血或石榴花的顏色) red 2 (象徵順利、成功或受人重視、歡迎) symbol of success lu...
- 素 : Ⅰ形容詞1 (本色; 白色) white 2 (顏色單純) plain; simple; quiet 3 (本來的; 原有的) native Ⅱ名...
- 鏈 : Ⅰ名詞1. (鏈子) chain Ⅱ動詞(用鏈栓住) chain; enchain Ⅲ量詞(計量海洋上距離的長度單位) cable length
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 黴素 : mycin
- 黴菌 : [微生物學] mould; mycete; mucedine
-
It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ) it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中,細菌污染是影響疫苗質量和產量的關鍵性因素,筆者通過了兩年左右的時間,選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份,進行細菌學檢查,並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗,結果表明:我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌,潛在威脅是桿菌和鏈球菌;強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果,可作為疫苗生產后備抗菌手段參考First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后,考察了影響兩親株原生質體形成和再生的主要因素,確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件:前者用s培養基,在28 、 220r . min ~ ( - 1 )培養24h后,用3mg ml的溶菌酶在32恆溫酶解50 60min ,得到的原生質體在乾燥的r5培養基上28倒置培養5 6天,可得到再生率在20左右的再生菌落;後者採用二級菌絲培養,用1mg ml的溶菌酶在37恆溫酶解1h左右,得到的原生質體也在乾燥的r5培養基上28倒置培養5天,即可得到再生率在20左右的再生菌。But in s. mycarofaciens, there is no distinctive propionate kinase, so that the utilization rate of propionic acid is lower than that of acetic acid. for this reason, it is hoped that the higher producers would be obtained by protoplast interspecies fusion between s. mycarofaciens and s. erythreus, which would transfer propionate kinase of s. erythreus to s. mycarofaciens. therefore, the fusion cells could use propionic acid as precursor in synthesis of mdma _ ( 1 ), which would reduce the production costs
根據文獻報道,紅黴素鏈黴菌中的丙酸激酶對丙酸的利用率是對乙酸的利用率的13倍;而在生米卡鏈黴菌中,無特異的丙酸激酶,菌體對丙酸的利用低於對乙酸的利用,因此希望利用原生質體種間融合的方法將紅黴素鏈黴菌的丙酸激酶基因轉移到生米卡鏈黴菌中,從而使融合子能夠利用丙酸鹽作為合成mdma _ 1的前體,提高mdma _ 1的產量,降低生產成本。464 and erythromycin producer s. erythreus as original strains, studies were carried out for researching higher producers and enhancing content of midecamycin ( mdm ) ai, which could use propionate as precursor to reduce production costs. according to the literature, the utilization rate of propionic acid is thirteen times to that of acetic acid by propionate kinase in s. erythreus
本論文主要研究了生米卡鏈黴菌與紅黴素鏈黴菌原生質體種間融合育種,以提高麥迪黴素a _ 1 ( mdma _ 1 )的組分,增加其對丙酸鹽的利用度,降低生產成本。分享友人