純培養分離 的英文怎麼說

中文拼音 [chúnpéiyǎngfēnlí]
純培養分離 英文
isolation of pure culture
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  1. Wild filamentous fungi obtained from natural fermented millet catsup were identified as aspergillus oryzae by morphology after purifying iteratively

    摘要反復純化培養自然發酵的粟米醬中分離的野生絲狀真菌，經形態學鑒定，確認其為米曲?菌群的米曲?菌。
  2. Eng. ) preparation of media, culture of bacteria, isolation and purification of bacteria, preservation of bacterial strain, gram stain and observation of bacterial strain, biochemical test, growth curve, preparation and analysis of bacterial dna

    中）培養基的制備，菌株的培養，菌株的分離及純化，劃線分離法，及連續稀釋法，菌株的保存，菌株的格蘭氏染色法，菌株生化反應的測試，菌株生長曲線的測定，菌株的染色體dna之制備及分析。
  3. Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba

    擴張床吸附（ eba ）技術是一種新型的生化分離技術，它集成了固液分離、濃縮和初期純化於一步單元操作之中，可以直接從含有細胞和細胞碎片的發酵液或培養液中提取目標蛋白，而不必事先除去懸浮的固體顆粒。
  4. The isolation and cufure of the ductal cells of smg in vitro

    實驗結果一、領下腺細胞的分離純化與體外培養、傳代。
  5. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  6. Exosomes derived from human intestinal epithelial cell ( iec ) could be released from apical and basolateral sides and expressed mhc class i molecules, cd26, cd63 in basal conditions besides mhc class ii in inflammatory conditions. the data showed exosomes might be a mechanism of oral tolerance. exosome - like vesicles isolated from rat iec line, which were pulsed by antigens, were shown to induce antigen - spec

    模型的建立和exosomes的分離與純化我們利用一種c57bl / 6小鼠的3lllewis肺癌細胞系作模型，將3ll細胞皮下注射到c57bl / 6小鼠，待3周時無菌取出腫瘤組織，剪碎後用dmem加10 %的血清在37 ， 5 %的czo中培養， 30小時后收集培養上清。
  7. The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum

    應用胰酶消化法進行頜下腺細胞的分離、純化及原代培養、傳代培養。
  8. Crumbs of nails were isolated from onychomycosis suffers, koh was chosen to be solvent, nail fungi were examined by microscopy, cultured in sabourud, purified by method of concentration grads and method of exsecting cusp of mycelia. finally, sixteen purified strains were ready

    從甲病患者病甲中分離出甲碎屑，選用koh為浮載劑，直接鏡檢，採用沙堡氏培養基對它們進行分離培養，濃度梯度法和切取菌絲尖端生長法對菌種進行純化，得到純化后的菌株16株。
  9. So the preparation of a pure culture involves the isolation of a given microorganism from a mixed natural microbial population.

    因此純培養制備要從混合的天然微生物群中分離出特定的微生物。
  10. In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method

    為研究外生菌根真菌本身對重金屬污染的耐性，比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法，以真菌生物量大小和分離難易程度為主要指標，篩選出液體靜置方法為最優方法。
  11. This method of isolation, cultivation and purification of the synovial cells in vitro is proved to be simple and feasible

    結論本研究建立了簡單易行的滑膜細胞分離、培養和純化方法。
  12. Results isolation, culture and purification of the mmscs typically, mmscs were isolated from bone marrow by their adherence to plastic and grew as fibroblastic cells that developed into visible symmetric colonies

    顯色15 30分鐘。結果1 mmscs分離、培養及純化用含10胎牛血清的dmem ? lg培養基在玻璃培養瓶中培養骨髓單個核細胞。
  13. Thirty - nine pure isolates and eighteen pure cultures were obtained, of which five pure cultures were registered in the european bank of the glomales ( beg ). a germplasm stock was established for storage of the resources in the target area

    原始樣品經過誘集培養、分離純化后，得到39個純凈菌株， 18個純株，在歐洲菌藏中心登記5個菌株，在此基礎上初步建立了菌種保藏室。
  14. The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively

    主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
  15. 3 ) dispased cold digested single cell is the best to obtain pured epithelial cell line ; it is appropriate to obtain pured fibroblast cell line that repeated attachment and the pured method to their different sensibility to trypsin

    對于秦川牛皮膚上皮細胞與成纖維細胞的純化： dispase冷消化單細胞培養的效果最佳，可獲得純化的上皮細胞系，而根據二者對胰蛋白酶的敏感性不同的分離方法、反復貼壁法適宜於獲得純化的成纖維細胞系。
  16. After washing with reagent, adopt the newest purification technology source30rpc, sds - page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins. after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition. finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %

    結論:總之，通過對發酵罐中重組工程菌各種培養因素的研究，建立了一種高密度、高表達發酵工藝體系，為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究，建立了一種高效穩定、快速簡潔、易於放大的包涵體重組蛋白分離純化體系。
  17. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。
  18. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下： 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板培養基從土樣中篩選出102株酸性植酸酶產生菌，從中挑選出透明圈較大的菌株32株，經分離純化後分別進行搖瓶復篩， 28 、 220r / min發酵5天後，在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活，結果發現有3株菌產酶活性較高且產酶性能較為穩定。
  19. In the current study, we tried to isolate liver progenitor cells from retrorsine - treated mouse. after long - term culture and purification, the pure epithelial cell population was established in cobblestone fashion with high nuclear - to - cytoplasm ratios

    採用兩步膠原酶消化法從損傷后的再生肝中分離細胞，經過長期的培養和不斷的純化，最終在體外建立了形態均一的連續細胞系。
  20. The protein were first purified on the basis of the stimulatory effect on the basal release of gonadotropins by rat anterior - pituitary cells in culture

    最初是由於可以促進培養中的大鼠垂體前葉細胞分泌促性腺激素而被分離、純化的。
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