純培養法 的英文怎麼說

中文拼音 [chúnpéiyǎngfǎ]
純培養法 英文
pure culture method
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  1. The respiratory intensity of the contaminated soil decreased by 29. 93 % while ammonification and nitrification increased significantly than that of control soil. 2. extraction and purification of soil microbial total dna a method of extracting soil total dna was developed, and it can extract dna from g + bacteria

    二、土壤微生物總dna的提取和純化方法研究為了採用不依賴于培養的16srdna分析的方法研究有機磷農藥長期污染對土壤微生物群落結構的影響，建立了從土壤中提取總dna的方法，並通過改進使適合於對革蘭氏陽性菌的提取。
  2. Eng. ) preparation of media, culture of bacteria, isolation and purification of bacteria, preservation of bacterial strain, gram stain and observation of bacterial strain, biochemical test, growth curve, preparation and analysis of bacterial dna

    中）培養基的制備，菌株的培養，菌株的分離及純化，劃線分離法，及連續稀釋法，菌株的保存，菌株的格蘭氏染色法，菌株生化反應的測試，菌株生長曲線的測定，菌株的染色體dna之制備及分析。
  3. Spawn is necessary for both cultivation of edible fungi and tameness of wild mushroom. the reliable method of spawn identification is to produce carpophores by the culture, which is not only time - consuming for domestic fungi but also unuseful for untamed wild mushroom

    無論是食用菌的人工栽培，還是珍貴野生蘑菇的馴化研究，都需要制備菌種並對菌種的真偽進行鑒定，其中最可靠的鑒定方法是誘導純培養菌種產生賴以識別的子實體。
  4. The molecular library technology has special values compared with conventional techniques for serodiagnostic screening of antigens, especially when the pathogens are difficult to culture artificially or the specific antigen purification is hard

    特別是當病原體不易人工培養或難于制備純化抗原時，這種技術較傳統診斷抗原篩選方法具有明顯的優勢。
  5. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  6. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  7. The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum

    應用胰酶消化法進行頜下腺細胞的分離、純化及原代培養、傳代培養。
  8. Crumbs of nails were isolated from onychomycosis suffers, koh was chosen to be solvent, nail fungi were examined by microscopy, cultured in sabourud, purified by method of concentration grads and method of exsecting cusp of mycelia. finally, sixteen purified strains were ready

    從甲病患者病甲中分離出甲碎屑，選用koh為浮載劑，直接鏡檢，採用沙堡氏培養基對它們進行分離培養，濃度梯度法和切取菌絲尖端生長法對菌種進行純化，得到純化后的菌株16株。
  9. Chapter one has mainly introduced the development of the market of chinese car, and the theory of satisfaction to customer has carried on the survey. foreign study on customer satisfaction deepen and not thorough quite already, customer satisfaction research to accompany by enterprise management idea transformation of weight and produce. the basic goal that enterprises exist is to pursue the maximization of the profit or maximization of enterprise ' s value, satisfied with the centre theory to develop from theory of centre of output value to the customer progressively, just because the customer is satisfied with the appearance of the centre theory, have produced customer ' s satisfactory research

    我國的汽車市場已經步入了高速發展的快車道，市場競爭空前激烈，產品同質化越來越高、可替代性越來越強、服務模式也日趨同一化，顧客的消費行為亦由感性階段進入了理性階段，在這樣一個復雜、瞬息萬變的市場激烈競爭中，單純的產品策略、服務策略等營銷手段無疑已經無法被顧客識別，而以「顧客為中心」 ，不斷提高顧客滿意度、提升顧客忠誠度、培養獨特的品牌已經成為了轎車企業突圍的唯一出路。
  10. The aim of cesar ' s methods is to produce a calm and assertive human in order to bring about a calm and submissive state in the dogs - not submissive in a fearful sense, but simply to relinquish leadership to the humans and allow them to decide what rules, boundaries and limitations should be set

    凱薩的方法是要培養出冷靜果斷的領導人，以便在狗群中製造出冷靜果斷的氛圍並非畏縮地一味服從，而是單純地聽憑領導人決定應該遵守的規則，紀律與限制。
  11. In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method

    為研究外生菌根真菌本身對重金屬污染的耐性，比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法，以真菌生物量大小和分離難易程度為主要指標，篩選出液體靜置方法為最優方法。
  12. This method of isolation, cultivation and purification of the synovial cells in vitro is proved to be simple and feasible

    結論本研究建立了簡單易行的滑膜細胞分離、培養和純化方法。
  13. The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level

    抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。
  14. The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively

    主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
  15. 3 ) dispased cold digested single cell is the best to obtain pured epithelial cell line ; it is appropriate to obtain pured fibroblast cell line that repeated attachment and the pured method to their different sensibility to trypsin

    對于秦川牛皮膚上皮細胞與成纖維細胞的純化： dispase冷消化單細胞培養的效果最佳，可獲得純化的上皮細胞系，而根據二者對胰蛋白酶的敏感性不同的分離方法、反復貼壁法適宜於獲得純化的成纖維細胞系。
  16. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。
  17. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下： 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板培養基從土樣中篩選出102株酸性植酸酶產生菌，從中挑選出透明圈較大的菌株32株，經分離純化後分別進行搖瓶復篩， 28 、 220r / min發酵5天後，在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活，結果發現有3株菌產酶活性較高且產酶性能較為穩定。
  18. In the current study, we tried to isolate liver progenitor cells from retrorsine - treated mouse. after long - term culture and purification, the pure epithelial cell population was established in cobblestone fashion with high nuclear - to - cytoplasm ratios

    採用兩步膠原酶消化法從損傷后的再生肝中分離細胞，經過長期的培養和不斷的純化，最終在體外建立了形態均一的連續細胞系。
  19. Human granulosa cells isolated from follicular fluid of 16 patients received ivf or icsi were cultured in tcm199 medium. granulosa cells were examined to detect fshr by immunobiochemistry assay and rt - pcr. 3

    對16例接受體外受精患者卵泡液中分離的卵巢顆粒細胞進行體外培養，利用免疫組化法和rt pcr法檢測顆粒細胞的fshr的蛋白表達和mrna的表達，鑒定體外培養顆粒細胞的純度； 3
  20. The molecular approach to detecting peptide hormones using cdna probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them

    採用cdna探子來測定肽激素的這一分子生物學方法同時也應該比免疫學的方法速度來得快，因為對于免疫的方法來說，需耗費好幾年枯燥的提純進程，方能將肽素分離了出來，然後再培養出針對它們的抗血清。
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