純蛋白比 的英文怎麼說

中文拼音 [chúndànbáibǐ]
純蛋白比 英文
npr=net protein ratio
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 比 : Ⅰ動詞1 （比較; 較量高下、 長短、距離、好壞等） compare; compete; contrast; match; emulate 2 （比...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  3. Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %

    本研究的主要結果和結論如下：一、經抗原分子設計，在430a自動肽合成儀上合成的新抗原p3 ，經質譜儀分析證明其荷質比與理論一致；純化后，其純度大於95 ；將新抗原與klh偶連后免疫小鼠，經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。
  4. The growth of f - 16 and the production of enzyme were affected by microbial medium, including c source, n source, mineral, initial ph of medium, rotating rate, culture time and culture temperature. the results showed that the optimal n sources were peptone, corn steep liquor and yeast extract ; the optimal c sources were sucrose, glucose and maltose ; the optimal minerals were mgso4 7h2o, khpcu and cuso4 5h2o

    實驗表明，氮源中蛋白腖、玉米漿、酵母粉比較好；碳源中蔗糖、葡萄糖、麥芽糖這三種糖對產酶和生物量提高效果顯著；無機鹽中mgso _ 4 ? 7h _ 2o ， k _ 2hpo _ 4 ， cuso _ 4 ? 5h _ 2o對產酯酶的酶活及其反應后所得水解液的光學純度有較好的作用。
  5. Comparison of the entire derived peptide sequence with other serine protease revealed significant homology, especially with a reported gene from another b. pumilus strain tyo - 67 ( 99 % homology )

    將克隆到的堿性蛋白酶基因（命名為ap ）編碼的成熟蛋白n一端序列與純化的脫毛蛋白酶n一端序列進行比較，二者完全相同。
  6. According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat, we designed primers and got the structure gene successfully. by 3 ' - race method combined with nested pcr, the 3 ' - end nuclear acid sequence was also obtained ; in additon, for the 5 ' - end sequence, we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer, and till now, partial 5 ' - end sequence has been amplified as well

    本研究根據先前分離純化所得天然tb22kda蛋白經maldi - tof - ms （質譜法）測得的氨基酸序列和文獻報道的過敏蛋白核苷酸序列設計引物，擴增克隆了該過敏蛋白結構基因的編碼序列；根據測得的序列設計特異性引物，並利用3 』 - race方法結合巢式pcr擴增得到基因的3 』末端；依據同源性比較的結果選用一段保守序列為5 』引物，並根據結構基因內部序列設計3 』特異性引物，進一步獲得了該基因5 』端的部分序列。
  7. 12 % sds - polyacrylamide gel electrophoresis ( sds - page ) was employed to analyze the purity and the protein concentration was measured by the bradford method

    將蛋白純化後分別測定rl ， a和rta一yqrl蛋白對各種細胞的毒性作用，比較ics 。
  8. The yeast recombinant expression vector pplc3. 5k - ndrg2 was constructed, then the ndrg2 protein was expressed in soluable form in pichia. after purification by metal chelate affinity chromatography, we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future

    為研究ndrgz的結構與功能，構建了ndrgz的酵母融合表達質粒，並在比赤酵母中得到可溶形式的表達；利用金屬螫合親合層析純化出了6his融合的ndrgz蛋白。
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