細分篩 的英文怎麼說
中文拼音 [xìfēnshāi]
細分篩
英文
sifting screen-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation
簡要綜述了生物技術在水稻遺傳育種中的應用，主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。In the research field of food preservation, an intense interest for scientists is focused on the isolation of antimicrobial substances from microbial metabolites. natural biopreservatives derived from traditional fermented foods are generally recognized as safe. in this paper, one strain screened for its safety and broad antimicrobial spectrum and its antimicrobial substance - - bacteriocin are studied in detail
本文從傳統的發酵食品中篩選出一株安全、具有廣譜抗菌活性的芽孢桿菌，並對其抗菌活性物質細菌素進行分析，為微生物天然生物防腐劑的開發以及在食品中的應用提供理論依據。Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library
我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法，獲得了對酵母細胞即無毒性作用，又沒有自激活作用的「誘餌」 ，通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。The pollen can then be separated from the rest of the catkin by sieving through cheesecloth or fine screen.
以粗紗布或細篩過篩，便可將花粉同雄花序的其它部分分開。Both intraductal and infiltrating ductal carcinoma are seen here. note the intraductal component in the center with cribriform pattern and prominent microcalcifications. surrounding this are infiltrating carcinoma cells
同時看到導管內癌與浸潤性導管癌。值得注意的是中心處篩型且有明顯微鈣化的導管內癌組成部分。其周圍是浸潤性癌細胞。Whole machine with simply structure, crush room and material feeder for easy clean and dismantle. the touching parts of material, all adopt stainless steel, to acid endurable, erosion endurable. the machine with balance operation, low noise, good effects on crush and low energy consumption
本機有加料機構及粉碎機構組成，結構簡單，清洗方便，與物料接觸部分全部採用不銹鋼製造，能耐酸、堿侵蝕、噪音低、效果好、能耗低、粉碎刀片可四面更換，並可根據物料的不同性能及粗細通過更換篩網布獲得理想的效果。Cyclosis ( cytoplasmic streaming ) the streaming of cytoplasm in a circular motion around the cell observed in some plants, particularly young sieve tube elements
胞質環流（胞質運動） ：在一些植物細胞特別是在幼篩管分子中觀察到的細胞質圍繞細胞的環形流動的現象。Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control
選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為pAt first, this paper analyzes the factors of water - sand influencing water level of yellow river and the feasibility just using the factors of water - sand to study water level, and collects the corresponding data ; secondly, because there are strong nonlinear relation in the corresponding data, by meticulous theory analysis, this paper integrates basic nonlinear analysis method, theory of random analysis, method of least squares and so on. it puts forward a method which can get the high accuracy simulation of the data, perfects the multi - factor analysis of variable ( over three factors ) of the statistic ; thirdly, it applies the method to the approximation of corresponding water level process which belong to the capacity of sand of middle - high and middle - low, and get the high - accuracy simulation about the typical nonlinear relation ; at last, this paper definitudes the main influence mode that the capacity of sand. it mainly unite with other factors to work on the water level in the yellow river lower reaches ; mor eover, this paper analyzes the difficult point and the direction of improvement to realize the accuracy forecasting of the flood level of erodible - bed channel
首先，系統分析了影響黃河水位的水沙因素，及僅用水沙因素有效研究水位的可行性，並按變量對應思想採集它們的相應數據；其次，由於相應水位過程數據中含極強的非線性關系，本論文經細致的理論分析，將基本的非線性分析方法、統計建模方法、隨機分析理論、最小均方誤差原則等等數學理論及方法有機揉合，提出了能有效實現這類數據高精度擬合的分層篩選法，並改進了統計學中多因子（三個以上）方差分析法；再次，將這一方法用於黃河中高及中低含沙類洪水相應水位過程的擬合，實現了這一典型非線性關系的高精度擬合，各年汛期上下游相應洪水位過程的擬合誤差都較小；最後，明確黃河下游含沙量對水位的主要影響方式，即含沙量主要是與其它因素聯合對水位作用；另外分析了要實現變動河床洪水位過程準確預報的困難所在及改進方向。Determination of degree of fineness of dyes by screen analysis
染料篩分細度的測定方法Isolation and screen of microbial cell flocculent
微生物細胞絮凝物的分離篩選Abstract : selective mechanisms exist in organism and biosphere, which select the cells with less dna injuy and reproduces them. there are two models ; mechanisms 1 and mechanisms 2, represented respectively b bacteria and mechanolytes of human beings. cells with mechanisms 1 ( e. g. bacteria, generative cells, cancer cells ) can reproduce infinitely, while somatic cells can not. there are deep - going reasons behind the two categories of selective mechanisms. the canceration of the cells is a degenerating process from mechanisms 2 to mechanisms 1
摘要生物界存在一類篩選機制，它將dna損傷較小的細胞篩選出來，產生後代.主要有兩類模式機制1和機制2 ，分別以細菌和人的成纖維細胞為代表，那些具有篩選機制1的細胞（如:細菌、生殖細胞、癌細胞）能無限傳代，而體細胞則不能.這兩類篩選機制背後有更深刻的原因，而細胞的癌變正是從機制2退化為機制1的過程。2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent
取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞，再常規融合和篩選，三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。Sp2 / 0 myeloma cells are fused with spleen cells of balb / c mice ( the ratio is 1 : 10 ) immunized with at - by routine method. positive clones are detected by elisa
Sp2 / 0細胞和免疫脾細胞按1 ： 10比例融合，融合劑peg分子量為1500 ，陽性克隆篩選採用三步進行。For the purpose of exploiting the affluent resource of traditional chinese medicines, rat pheochromocytoma pc 12 cells were used as a model to screen the neuroactive components from natural products. most of all, the effect of it on inducing the differentiation of pc 12 cells and / or execute the neuroprotective effect challenged by serum - deprivation and oxidative pressure, and its mechanism were studied in this paper
本文以利用我國豐富的天然藥物資源為目的，用大鼠嗜鉻神經瘤細胞pc12作為模型，篩選能模擬ngf的功能誘導pc12細胞分化、對神經細胞有一定保護作用的天然藥物，並對其作用機制進行初步探索。Conclusion : the system not only could pick upon the op timum strain on fixed culture medium or the optimum substrate to special strain fleetly but also could be used to evaluate the microbe growth character apace, monitor z ymogen upgrowth in fermentor and determine total number of bacteria in fresh cre amery rapidly
結論：研製的生物傳感器系統可用於菌種和培養基組分的快速篩選、微生物活細胞生長特性的快速評估，還可用於發酵罐中發酵菌的生長監測及新鮮牛奶中雜菌總數的快速測定。In aqueous solution, interaction between crude venom and different bacteria including gram - positive bacteria, i. e. streptococcus mutans, streptococcus aureus, and gram - negative bacteria, i. e. e. colik12, was investigated in detail. molecule weights of multiple proteins were identified as 110kda, 47kda, 24kda, 23kda and 15kda by sds - page, respectively. it had little influence on extraction of snake venom proteins by the same cells of streptococcus mutans even used for 10 cycles
其次，在利用s . mutans分離細菌特異性抗體的基礎上，首次利用細菌篩選作用蛋白，將革蘭氏陽性菌（ s . mutans和s . aureus ）和革蘭氏陰性菌（ e . colik12 ）分別與蘄蛇蛇毒相互作用，發現有五種蛇毒蛋白能夠與細菌相互作用，其分子量分別為110kda 、 47kda 、 24kda 、 23kda和15kda ；同時表明s . mutans菌體能夠多次與蛇毒相互作用，並且對作用蛋白的結合沒有影響。Zy series vibrated filter is one of precised powder griddle, low noise, high efficiency, need only 3 ~ 5 minutes to rapidly replace the griddle, all closed structure. used to filter particles, powder and mucilage
Zy系列三次元振動篩是一種高精度細粉篩分機械，具有噪音低、效率高、快速換網只需3 ~ 5分鐘，全封閉結構，適用於粒、粉、粘液的篩分過濾。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。分享友人