細胞周期時間 的英文怎麼說
中文拼音 [xìbāozhōuqīshíjiān]
細胞周期時間
英文
cell cycle time- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 周 : Ⅰ名詞1 (圈子; 周圍) circumference; periphery; circuit 2 (星期) week 3 [電學] (周波的簡稱) c...
- 期 : 期名詞[書面語]1. (一周年) a full year; anniversary 2. (一整月) a full month
- 時 : shí]Ⅰ名1 (比較長的一段時間)time; times; days:當時at that time; in those days; 古時 ancient tim...
- 間 : 間Ⅰ名詞1 (中間) between; among 2 (一定的空間或時間里) with a definite time or space 3 (一間...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
- 時間 : time; hour; 北京時間十九點整19 hours beijing time; 上課時間school hours; 時間與空間 time and spac...
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Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen
試驗二,不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一,進入正式試驗期后,在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠,稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液,並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血,姬姆薩-瑞氏染色,計算t淋巴細胞轉化率,計算免疫器官指數,測定肝臟、脾臟銅含量。Constructing quantitative model with ordinary differential equations for the cell - cycle control system, it is appropriate to use ordinary differential equations ( odes ), because molecular diffusion, transcription, translation and membrane transport seem to be fast ( a matter of seconds ) compared with the duration of the cell cycle ( hours ). spatial localization of reactions can be handled by compart - mental modelling, in the spirit of pharmacokinetics
對于這樣的細胞周期控制系統,應用常微分方程是適合的,因為比起細胞周期的時間(以小時計)來,分子擴散,轉錄,翻譯和膜運輸是很快的(以秒計應用藥物動力學的區域化模型的方法,可以處理反應的空間分佈。Germinal vesicle ( gv ) transfer is a technique developed in recent years, which may be a valuable research procedure to study cell cycle regulation and characterize the cytoplasmic - nuclear interplay during maturation and fertilization of oocytes
生發泡( gv )移植是最近幾年才建立起來的一種技術。通過gv移植可以研究細胞周期調控、成熟及受精時細胞核與細胞質之間的相互作用。At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage
在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化,結果表明,分裂期細胞總體熒光強度強于間期細胞;而對同一細胞內熒光強度的分析說明,間期細胞的熒光主要分佈於胞質中,細胞核內則分佈較少;而正在進行有絲分裂的細胞內熒光主要集中於赤道板處;剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。In xenopus embryonic extracts camp concentration and pka activity also oscillations accompany with the cell cycle, low in mitosis and increased during m / g1 transition, keep high until next mitosis, and the mechanism concern with the cdc25 phosphorylation state and cyclin b concentration. mouse fertilized egg is the most simple and natural model for cell cycle that near to human, but little is know about pka on mpf also pka on mitosis
通過對非洲爪贍胚胎細胞周期的研究發現jka活性也隨細胞周期變化而波動,在m期出現時pka活性下降,而m間期轉換時pka活性達最大值,一直維持高值直到下一個m期出現,其對mpf活性調節與cdc25磷酸化狀態及周期素b表達有關。Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e. metck darmstadt methods superovulation and collection of eggs for superovulation, female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ), and after 46 - 48 hours with human chorionic ginadotropin. ( hcg ). one - cell fertilized eggs were collected on the next day from oviduct of females
取4一5周齡成熟雌性昆明系小白鼠,腹腔注射pmsg (孕馬血清促性腺激素) 10iu , 46一48小時后腹腔注射hcg (人絨毛膜促性腺激素) 1oiu ,將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配,次日檢察陰栓,將查到陰栓的雌鼠處死,取輸卵管于mz培養液中,解剖鏡下撕開壺腹,釋放細胞團,然後用300林歲nil透明質酸酶消化去除顆粒細胞,口控吸管將卵細胞在m :中反復清洗,然後置於孵箱中,根據時間點收集g2期細胞。Because selective period of chloroplast transformation was longer than that of the nuclear transformation, only the several weakly regenerated plants with spectinomynic resistantance were obtained
由於葉綠體轉化植株生長較為緩慢,篩選周期比細胞核轉化長,並且一段時間后會出現白化現象。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系,本實驗研究分為三個部分: ( 1 ) axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建; ( 2 )探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理,並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響; ( 3 ) axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下: 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ,並將其構建入真核表達載體中,編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。Rapid responses occured when cells were exposed to genotoxic stresses : one is initiating dna damage checkpoint that leads to arrest in gl, in order to provide the cell with sufficient time to repair damaged dna, or leads to arrest in g2, in order to avoid defective chromosome happening mitosis. on the other hand, if damage to the genome is excessive, the cells initiating apoptosis, when damages cannot be repaired
這是因為細胞在受到諸如電離輻射等基因毒應激損傷時,細胞最先作出的細胞學反應:一,起始dnadamagecheckpoint使細胞周期阻滯( arrest )在g1期,贏得時間識別與修復損傷,避免損傷的dna進行錯誤的復制,或和阻滯在g2期,避免缺陷的染色體進一步分裂;二,損傷嚴重而修復無望啟動細胞死亡程序,清除那些帶有病變傾向的細胞危害機體。分享友人