細胞性抗體 的英文怎麼說
中文拼音 [xìbāoxìngkàngtǐ]
細胞性抗體
英文
cellular antibody- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
-
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent
取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞,再常規融合和篩選,三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。Advances on the pathogenesis of anti - neutrophil cytoplasmic antibody associated vasculitis
抗中性粒細胞胞漿抗體相關性血管炎研究進展This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。The bispecific antibody tilers of ascites fluid and culture supernatant of 3 - hn are 1 : 16000 and 1 : 64. at the same time the anti - p24 antibody and the anti - human rbc type a antibody in the ascites fluid of hybrid - hybridoma 3 - hn are tested, and their titer is 1 : 800 and 1 : 16000 respectively
為89士9條j十; ;的腹水型和上清型雙特異性抗體的效價分別為1 : 16000和1 : 64 ,同時測定腹水型抗體抗p24抗體活性及抗人a型紅細胞凝集抗體活性,效價分別為1 : 800和1 : 16000 。Vasculitis associated with propylthiouracil - induced antineutrophil cytoplasmic antibody is not drug induced lupus
丙基硫氧嘧啶引起抗中性粒細胞胞質抗體相關小血管炎並非藥物性狼瘡2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody
選其中一株3 - h _ ( 11 )做雜交-雜交瘤細胞染色體計數,同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數:制備腹水型和上清型抗體,用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體,即紅細胞固相吸附法測雙特異中文摘要性抗體,紅細胞凝集試驗測單特異性抗人a型紅細胞抗體,間接elisa法測抗p24抗體;用腹水型抗體做耐熱性及耐凍融實驗。Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but
通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價,高親和力,較好特異性的抗mt單克隆抗體,最後獲得了5株單克隆細胞株,都能分泌針對mt的特異性抗體,建立了選擇性基團免疫分析法,用制備的抗體測定了人血清中mt的含量,作了mt的抑制標準曲線,其抑制范圍從50pg ? 30ng ,半抑制量為1These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr. it seems that the increased 1, 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n, while the decrease of this branch may prevent these processes
用特異性抗體結合westemblot結果發現,正義或反義gnt一vcdna的轉染並不引起pkb 、 p44 / 42mapk和mek蛋白質表達的變化,而gntv一s / h 」 21細胞pkbt308 、 5473位點磷酸化和免疫沉澱pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化為指標的pkb的活性都較mock細胞增加, gntv一as / h7721細胞中這些指標的變化則相反。Most recent evidence suggests that physiologic apoptosis serves as the mechanism to translocate intracellular antigens to the cell surface ( 7 ), while other reports have indicated that the specific antibodies may act first by inhibiting inward calcium fluxes across the target cell membranes, producing the arrythmia s ( 8 )
最近的證據表明,生理性凋亡可能促使細胞內抗原移位到細胞表面[ 7 ] ;但也有研究表明,特定的抗體可能首先通過抑制靶細胞膜的鈣內流,從而導致心律失常[ 8 ] 。Conclusions : this study opened a new field of application of antigen - pulsed dcs for prophylactic purposes when adequate levels of protective antibody cannot be induced by traditional vaccination approaches
結論:該研究開辟了負載抗原的樹狀突細胞、新的、作為預防用的應用領域,當足夠的保護性抗體水平通過傳統的接種方法不能被誘導時。This bispecific antibody companied with another anti - p24 monospecific monoclonal antibody can build a new way of indirect rbc agglutination to detect p24 antigen, and this new way is preliminarily applied to detect serial diluted p24 to determine the sensitivity
用雙特異性抗體致敏人a型紅細胞做指示劑,以抗p24單特異件單克隆抗體9a7做交聯劑,建立間接血凝檢測p24抗原的新方法,並初步應用於檢測系列稀釋的p24抗原以確定測定的敏感性。Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce
( 3 )細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣,細胞核轉化獲得了生長良好的抗性萵苣植株,經pcr 、 pcr - southern 、 southern斑點雜交分析證實, adw基因已整合到萵苣基因組中。Then using ecbp21 antibody and immunogold transmission electron microscopy method, we studied the subcellular localization of ecbp21. the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica. these results indicated that ecbp21 mainly localized in cell wall, which provide a direct evidence of the extracellular existence of ecbp21. furthermore, using ecbp21 antibody and immunohistochemical method, we studied the organic specially distribution of ecbp21, the results indicated that ecbp21 distributed in all organize, but it distributed more in leave n flower rachis than in leafstalk and root
首先,構建了ecbp21表達載體,誘導了重組蛋白的表達,並通過膠回收法獲得了大量純化重組ecbp21蛋白,制備了高效價、高特異性抗體;隨后,利用ecbp21抗體,結合免疫膠體金電鏡定位技術進行了ecbp21亞細胞定位研究,結果顯示:在白芷愈傷組織細胞和花序軸細胞中金顆粒主要分佈在細胞壁區域,而在細胞內未發現或僅有少量金顆粒分佈,表明ecbp21蛋白主要定位於細胞壁區域,這為細胞外cambp ( ecbp21 )的胞外存在提供了直接證據:進一步,利用ecbp21抗體,通過免疫組織化學分析研究了ecbp21組織特異性分佈狀況,結果表明ecbp21在白芷各組織中均有分佈,但在葉、花、花序軸中分佈較多,而在葉柄、根中分佈較少。A positive correlation of scc ag with the percentage of body surface and psoriasis area and severity index ( pasi ) score was found
鱗狀細胞癌抗原與身體表面受侵犯的百分比及乾癬區域嚴重度指標呈現正相關性。To investigate the potential function of nogo - a involved in neuronal development and differentiation, this study mainly used hippocampal neuron culture model, both in conventional and low - density condition, and immunohistochernical and western blot techniques. nogo - a expression pattern in hippocampal neurons at different stages and its subcellular distribution were explored by using specific antibody raised against nogo - a. pc12, 3t3 cells were also cultured as controls
為探討nogo a在神經元中的表達與神經元發育分化的關系,本研究藉助了海馬神經元常規培養方法和低密度條件下培養方法,應用針對nogo a的特異性抗體,採用免疫熒光細胞化學染色和westernblot方法,觀察了nogo a在體外培養的大鼠海馬神經元表達分佈模式,和不同培養時間nogo a亞細胞分佈的變化。Therefore the hybrid - hybridoma can secrete not only bispecific antibody but also anti - human rbc type a monospecific antibody and probably anti - p24 monospecific antibody. the mixed antibodies purified by ammonium sulfate is further purified by p24 affinity chromatography. the unsp
這樣,分離出雙特異性抗體,並證實雜交雜交瘤除分泌抗p24和人紅細胞的雙特異性抗體外,還分泌抗p24單特異性抗體和抗人a型紅細胞單特異性抗體。The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively
方法是先用硫酸綏鹽析,再過親和層析柱,即33飽和硫酸按沉澱后,透祈過夜,上p24抗原做配體的濱化氰活化的sepharose4b柱,流洗掉雜蛋白和抗人紅細胞單特異性抗體后,再洗脫並收集雙特異性抗體。Objection : this study is to prepare a kind of bispecific antibody of anti - hiv p24 antigen and anti - human rbc type a antigen, and to build a new way to detect hiv p24 antigen with this bispecific antibody
目的:制備抗hivp24和抗人a型紅細胞雙特異性抗體,建立檢測hivp24抗原的新方法。Preparation and characterization of non - agglutinating mabs against membrane antigen on human erythrocytes
抗人紅細胞膜抗原非凝集型單克隆抗體的研製及特性鑒定Second, the ecbp21 was localized in the cell wall area by immuno - gold electron microscopy and by using gfp as a reporter
Ecbp21亞細胞定位:以重組ecbp21蛋白為抗原制備了特異性抗體。分享友人