細胞性狀轉化 的英文怎麼說

中文拼音 [xìbāoxìngzhuàngzhuǎnhuà]
細胞性狀轉化 英文
cellular transformation
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 狀 : Ⅰ名詞1 （形狀） form; shape 2 （情況） state; condition; situation; circumstances 3 （陳述事件或...
  • 轉 : 轉構詞成分。
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. Infectious agents such as human papillomavirus can lead to cellular transformation as well

    感染性因素如人類乳頭狀瘤病毒能導致細胞轉化。
  2. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  3. In xenopus embryonic extracts camp concentration and pka activity also oscillations accompany with the cell cycle, low in mitosis and increased during m / g1 transition, keep high until next mitosis, and the mechanism concern with the cdc25 phosphorylation state and cyclin b concentration. mouse fertilized egg is the most simple and natural model for cell cycle that near to human, but little is know about pka on mpf also pka on mitosis

    通過對非洲爪贍胚胎細胞周期的研究發現jka活性也隨細胞周期變化而波動，在m期出現時pka活性下降，而m間期轉換時pka活性達最大值，一直維持高值直到下一個m期出現，其對mpf活性調節與cdc25磷酸化狀態及周期素b表達有關。
  4. Gonadal development and fecundity are affected by certain essential dietary nutrients, especially essential amino acids, essential fatty acids, and vitamins etc. thus, during the last two decades, more attention has been paid to the optimal level of different nutrients in broodstock diets. it is well known that protein is a kind of indispensable nutrient because of its important physiological function on body structure and supplying energy to the growth and development of shrimp and crab. protein and amino acids of broodstock diet have been identified as major dietary factors that determine successful reproduction and survival of offspring

    而這些問題的解決則要求對中華絨螯蟹生殖過程中的攝食和消化生理機制，親體性成熟期對各種必需營養素（如必需氨基酸、必需脂肪酸和維生素等）的需求，卵細胞發生過程中營養物質的合成、轉運及貯存規律，以及在受精卵、胚胎和后續以內源性營養為主的?狀幼體發育階段對卵黃磷蛋白的消耗和利用模式等環節進行較為深入的研究，在全面系統地了解中華絨螯蟹的生殖營養生理的基礎上，探討親體生殖營養對卵質、孵化及后續幼體生長發育的影響。
  5. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  6. Serous cortisol content in each group increased, while cortisol content in group i was higher than that in each other group ( p < 0. 05 ). serous - ep content in each group increased and then decreased gradually, but - ep content in group iv has a significant increased at half an hour following incision ( p < 0. 05 ). conclusions canine immune functions are suppressed by operation with different anesthetic techniques to different degree, but resume at 15th day after operation. there is an interacting and interadjusting mechanism among the cns ( central nerve system ), the endocrine system and the immune system

    結果各組在麻醉手術后4h及術后1d白細胞總數、嗜中性分葉狀粒細胞數顯著增加（ p 0 . 05 ） ，淋巴細胞數減少；血清球蛋白含量先降低后升高；組rbc ? c _ 3br花環率先降低再升高后降低，其它組均先降低； t淋巴細胞轉化率均呈現不同程度的降低，在術后7d左右恢復正常；血漿皮質醇濃度先升高后降低，但組皮質醇含量在切皮后30min較其它組上升顯著（ p 0 . 05 ） ；血漿- ep濃度先升高后降低，但組- ep含量在切皮后30min較其它組上升顯著（ p 0 . 05 ） 。
  7. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  8. Aralia elate ( miq. ) seem is a kind of uncultivated vegetable from the mountain changbai. the process of somatic embryogenesis includes morphogenesis stage of calli, conversion stage of embryonic calli, embryogenetic stage and regeneration stage of plant in aralia elate (

    本論文採用sds -聚丙烯酰胺凝膠電泳技術，對龍芽?木體細胞胚發生過程中愈傷組織形態建成、胚性愈傷轉化、胚狀體發育和植株再生四個階段的可溶性蛋白的動態變化進行分析。
  9. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  10. The results suggested : the differential gene expression in the adrenal gland under acute hyperglycemia / hypoglycemia was related to blood glucose ; these genes might regulate blood glucose by influencing the metabolism and secretion of hormones, cytoskeleton constitution and signal transduction in the adrenal gland ; bbs4 gene might participate in glucoregulation via the activities of adrenal medulla ; the differential genes expressed under acute vs chronic hyperglycemia might play a basic role of the structural and functional changes in the adrenal gland, lead to the process of chronic complications of diabetes or accommodate to the status of hyperglycemia

    結果提示：腎上腺基因差異性表達和血糖變化有關；在急性高低血糖情況下，這些基因可能通過影響腎上腺內激素的分泌和代謝、細胞骨架構成和信號轉導來調節血糖； bbs4可能參與血糖調節，其作用可能和腎上腺髓質的活動有關；慢性高血糖情況下的腎上腺差異表達不同於急性高血糖，這種表達差異可能與腎上腺慢性的結構和機能改變相關，或參與了糖尿病慢性並發癥的發生發展、高血糖狀態的調節。
  11. Pbluebachisc - vp7 was identified and analped by compnding restriction endonuclease, pcr and nested pcr on the basis of the genetic sites of pbluebachisc, which was idenhfied as vp7 gene of prv hafied pbluebachisc - vp7 and barmidn - blue dna were cbeinfected insect cell sro and harvested mmbinan beculovirus 5d later identification with restriction empme and pcr showed vp7 gene has been arembwt comehy with baculovirus dna and namd a - l

    純化該重組質粒並與線性桿狀病毒dnabac - n - blue共轉染昆蟲細胞sr9 ， 5d后收獲重組病毒。重組桿狀病毒dna分子的pcr及酶切鑒定表明，獲得了prv - vp7基因與桿狀病毒dna的重組子，命名為a - 1代病毒。
  12. The results showed that the salt tolerance of transgenic strawberry was increased, the development of damage symptoms ( wilting ) caused by salt stress was delayed, and the recovery improved when salt stress conditions were removed

    結果表明， lea3基岡導入草譴細胞后，與對照相比轉化細胞再生植株抗鹽性明顯增強，並且在受到鹽脅迫時，轉化株萎蔫癥狀出現晚，受害程度輕，一口
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