細胞株蛋白 的英文怎麼說

中文拼音 [xìbāozhūdànbái]
細胞株蛋白 英文
cytoglobin
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 株 : Ⅰ名詞1. （露在地面上樹木的根和莖） root and stem of a tree above the ground 2. （植株） individual plant; plant Ⅱ量詞（棵）
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 細胞株 : cell line
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. With the successful expression of exogenous gene in plant. the advantage of plant expression system become a highlight increasingly. based on the successful expression of hbmp - 3m gene into tobacco, we maked the study o f transferring hbmp - 3m gene into tomato and hbmp - 3 gene into tobacco, in order to obtain tomato transgenic plant with hbmp - 3m gene and tobacco transgenic plant with hbmp - 3 gene, to establish basis for getting step farther of leaning the expression of hbmp - 3m gene and hbmp - 3 gene in plant, the difference between the product of hbmp - 3m gene and hbmp - 3 gene in plant and the active of expressible product

    隨著植物基因工程的迅猛發展，一些疫苗、抗體、細胞因子等異源蛋白在植物中的成功表達，植物表達系統的優點日益受到關注。本研究室在成功地將hbmp - 3成熟肽基因轉入煙草並檢測有目的蛋白表達的基礎上，進行hbmp - 3成熟肽基因轉化番茄和hbmp - 3全長基因轉化煙草的研究，以期獲得轉hbmp - 3成熟肽基因番茄和轉hbmp - 3全長基因煙草植株，為進一步研究hbmp - 3成熟肽基因和全長基因在植物體內的表達及區別奠定基礎。
  4. Signals were strong in the cell periphery of procambium, and longitudinal signals were stronger than lateral ones ; in root ground meristem cytoplasm, concentration in the perinuclear region was stronger than one in the cell periphery. in cell periphery of root ground meristem, distribution of actin mrna was heterogeneous, longitudinal signals were stronger than lateral ones ; in callus meristem cytoplasm, concentration in the perinuclear region was low ;

    這表明，從棉花愈傷組織薄壁細胞到鳥巢狀管胞團再到正常苗的過程中肌動蛋白mrna的分佈和濃度都有明顯的變化，而在這里愈傷組織在分化到鳥巢狀管胞團后就不再繼續發育，因而推測，肌動蛋白mrna分佈和濃度可能影響愈傷組織分化出正常的植株。
  5. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  6. Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst

    Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
  7. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  8. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  9. Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but

    通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1
  10. Recombinant adenovirus vectors carrying antisense mmp2 inhibit invasion of hcc cells in vitro

    重組腺病毒介導反義2型基質金屬蛋白酶抑制人肝癌細胞株浸潤的實驗研究
  11. 2. the functional of the purified trka extracellular domain proteins were tested using pc - 12 cells

    2 、用pc12細胞株測定腸ka膜外域各結構域重組蛋白的功能。
  12. The recombinant fasl - ecd was purified and its biological activity was analyzed on several cell lines and the most sensitive cell lines are selected. ( 2 ) using a computer program, a single short peptide is derived from antisense homology box of fas ligand and is chemically synthesized. ( 3 ) examine the apoptosis - inducing effect of the recombinant fasl - ecd and the ten - peptide on the most sensitive cell lines, and the relationship between them was analysed

    純化重組蛋白fasl - ecd ，並分析其生物學活性，篩選出對之最敏感的腫瘤細胞株； （ 2 ）根據生物信息學軟體分析結果，選取fasl胞外區256 - 265的十肽（ n ） - hlyvnvsels - （ c ）作為目標分子，委託生物公司合成該十肽； （ 3 ）分析fasl - ecd和十肽對最敏感腫瘤細胞的毒性作用，分析重組fasl一ecd及十膚作用的相關性。
  13. The ig class is iggi. mcab of at - iii provides very efficient tools for the detection and purification of at - iii

    獲得三株分泌抗人抗凝血酶的雜交瘤細胞株d5 、 e2 、 f6 ，免疫球蛋白的類型為igg _ 1型。
  14. Expression of human papillomavirus type 16 l1 and construction of hybridoma cell strain of human papillomavirus type 16 l1 monoclonal antibody

    1蛋白表達及其單克隆抗體雜交瘤細胞株的建立
  15. Then protein crystal of eif - 5a was observed using atomic force microscopy. further, the polyclonal and monoclonal antibodies were gained from immunized rabbits and rats. hypusine - contained eif - 5a plays a role in cell growth and differentiation

    用得到的重組蛋白分別免疫家兔和小鼠，獲得兔的抗血清和三株持續分泌單抗的雜交瘤細胞株，為進一步分析eif - 5a的功能提供了檢測手段。
  16. The optimal expressing time with the calcium phosphate method was 72h after transfection, and the transfection method of calcium phosphate is proved to be more effective than the lipofection method

    結果表明，采碩士論文戴君勇： k亞科動物的親緣關系及hsblys的真核表達用cos 7作為真核表達細胞株，其表達目的蛋白的最佳時間是72小時。
  17. Effect of lanthanium on proliferation and protein expression of liver cell line

    鑭對肝細胞株7701的增殖和對蛋白質表達的影響
  18. To make clear the hypothesis, a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study, respectively. we first studied the cellular localization of kvl. 2 and the co - localization of kvl. 2 protein and vegf receptors flk - 1 and flt - 1, observed the effect of mcao on kvl. 2 expression and phosphrylation in the rat brain in vivo, then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl. 2 in sh - sy5y cells. finally, in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl. 2 phosphorylation, we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl. 2 phosphorylation

    為了驗證上述假設，本文分別在整體和離體水平，採用大腦中動脈缺血（ middlecerebralarteryocclusion ， mcao ）和體外氧?糖剝奪（ hypoxiaandglucose - deprivation ， hgd ）缺血模型，首先了解了kv1 . 2蛋白的細胞定位及與vegf受體flk - 1和flt - 1的共存情況，觀察了整體mcao后缺血再灌不同時間大鼠腦內kv1 . 2蛋白的磷酸化水平變化，然後通過外源性給予vegf蛋白，在sh - sy5y細胞株上觀察其對缺血細胞存活率及kv1 . 2蛋白磷酸化水平的影響，最後利用vegf反義脫氧寡核苷酸（ oligodeoxynucleotide ， odn ）特異阻斷內源性vegf蛋白的表達，觀察內源性vegf蛋白在缺血細胞損傷及調節kv1 . 2蛋白磷酸化中的作用，以進一步明確vegf缺血保護效應與其調節kv1 . 2蛋白磷酸化之間的關系。
  19. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  20. The recombinant virus adhuctla4 - ig prepared in this study efliciently inltcted l - o2 " cells, and the infected cells expressed a - nd excreted soluble rccolllbina11t protein huctla4 - ig

    該重組病毒在體外能有效感染正常肝細胞株l - o2 ，受感染細胞能表達、分泌可溶性的重組融合蛋白huctla4 - ig ； 2
分享友人
分享到 Line

分享到臉書