細胞離心培養 的英文怎麼說

中文拼音 [xìbāolíxīnpéiyǎng]
細胞離心培養 英文
centrifugation onto cell culture
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  • 離心 : 1 （不是一條心） be at odds with the community or the leadership2 （離開中心） centrifugal; eccen...
  1. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  2. We can see a lot of new pictures of induced neuron - like cells, include neuron - like cells and glial - like cells, with the apparent nerve cells. material and methods rat bone marrow cell were collected, after sacrifice of a 3 months old wistar rat, from femurs and tibias by flushing the shaft with culture media ( dmem - 10 % fetal bovine serum ) using a syringe of 5ml

    無菌條件下取股骨和胚骨的骨髓b人snd含10胎牛血清的dmem培養液1000rpm離心10分鐘，吹打b人兩個培養瓶，置人37 j coh飽和濕度條件下培養，隔日更換培養液，當細胞達到70融合時，傳代。
  3. Cell morphology was similar no matter what method was used. percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) %. proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method. the maximal cell number was 4. 67 vs 4. 10 times that of the initial cell number at cell inoculation

    紅細胞裂解法較密度梯度離心法細胞貼壁較慢， 36小時才可見零星細胞貼壁，兩種方法細胞形態相似。活細胞百分比，紅細胞裂解法為（ gi士4 ） ，密度梯度離心治（ 83士5 ） ，有明顯差異。傳代培養： 2 6代范圍內，紅細胞裂解法較梯度離心法細胞增殖更旺盛（分別是按種時細胞數的4
  4. Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities

    因此將這些蛋白包被、固定到材料表面，觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節，並從以下方面進行探討： （ 1 ）採用不同原代細胞分離方法，研究其對mscs細胞的生物學特性影響。 （ 2 ）檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 （ 3 ）分別採用型膠原及纖維粘連蛋白（ fibronectin ， fn ）包被聚乙醇酸-乳酸共聚物（ poly （ 1actide - co - glycolide ） ， plga ）膜及多孔塊型plga材料，觀察細胞在單層或三維培養狀態下，型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。
  5. All the 4 extractions showed obvious pharmacological effects by decreasing the area of myocardial infraction of the model rats, increasing the amount of blood stream of the isolated guinea - pig hearts and reducing the beating rate and amplitude of cultured myocardial sheets of mice

    方法：採用冠狀動脈結扎致大鼠急性心肌缺血模型，觀察革葉獼猴桃果實70 %乙醇提取物、正己烷部位、氯仿部位及正丁醇部位對急性心肌梗塞范圍的影響，同時觀察上述部位對豚鼠離體心臟冠脈流量及小鼠胚胎心肌培養細胞功能的影響。
  6. Methods : gradient centrifugation and differential adherence were employed to isolate type pneumocytes from the lungs in the rats, proliferative activities were compared in the cultued cells from the young, adult and aged rats

    方法：應用梯度離心和貼壁培養分離大鼠肺泡型細胞，比較幼年、中年和老年大鼠培養肺泡型細胞的增殖能力。
  7. 3 after incubated with 5 - bromo - 2 - deoxyuridine ( brdu ) for 7 days, newborn cells derived from regenerative neurospheres were immunostained. 4 the cells were collected by centrifugation separation and put into poly - 1 - lysine - coated terasaki wells, which were randomly divided into one of three groups of 24 wells cells each

    4將傳代培養的神經球離心收集，轉移至24孔培養板，隨機分為3組，每組24孔細胞，置5 % coz 、 370c培養箱，連續培養7d ，觀察神經球的誘導分化。
  8. Research on the ratio method of measuring intracellular ca2 + concentration with ratio fluorescence imagemaster was reported in the last chapters, which include the folio wings : ( 1 ) the ratio experiment system of measuring intracellular ca concentration with ratio fluorescence imagemaster was established ; ( 2 ) the primary culture mode of suckling mouse cardiac muscle cell was established and the ca2 + concentration in suckling mouse cardiac muscle cell was measured. after adding ne ( norepinephrine ) to cardiac muscle cell, the relative fluorescence intensity was dramatic increased

    本文後半部分主要圍繞用比率熒光光譜儀測量細胞內游離ca ~ （ 2 + ）的研究進行了以下工作： （ 1 ）建立了比率熒光光譜儀測量細胞內游離鈣離子濃度的實驗系統； （ 2 ）建立了乳鼠心肌細胞原代培養的模型，並對培養細胞進行了細胞內ca ~ （ 2 + ）濃度的測量。
  9. Effect of et - 1 on intracellular free calcium in cultured neonatal myocardial cells

    內皮素對培養心肌細胞內游離鈣濃度的作用
  10. Image processing applying in analysis of motion features of cultured cardiac myocyte in rat

    圖像處理方法用於離體培養乳鼠心肌細胞動態特徵分析
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