細菌區系 的英文怎麼說
中文拼音 [xìjūnqūxì]
細菌區系
英文
bacterial flora-
The rhizosphere microflora dynamics of bacteria, actinomyces, fungi and four bacterial physiological groups of kentucky bluegrass under different quality of illumination were studied by adopting selective culture medium to explain scientifically response regular of this grass to different illumination condition
摘要研究了草地早熟禾在不同光照條件下其根際與非根際細菌、真菌、放線菌以及氨化細菌、硝化細菌、好氣性纖維素分解菌、固氮菌生理類群的區系動態變化，擬從根際土壤微生物數量變化方面來闡述草地早熟禾對不同光照條件的響應規律。Serious cross reation existed between v. albo - atrum and mv2, mv3, mv4. the other pathogen isolates v31 and v32 also had cross reactions, but the reaction was not serious. because limited number of pathogen isolates were selected, it could not prove that the selected immunogen was widely presentative, more pathogens isolates should be tested to verify the acquired hybridomas cells
5株單抗雜交瘤細胞中沒有一株具有種或屬的特異性，其中mv2在棉花黃萎病菌若干菌系間的檢測表明其能夠區分不同的致病類型； mv1和mv4組合檢測的結果基本上能將棉花大麗輪枝菌鑒定到種；黑白輪枝菌與mv2 ， mv3 ， mv4的交叉反應比較強烈，其他菌株v3 ， v32有個別的交叉反應，但不強烈Microbial populations and heavy metal tolerant bacteria in soils around a copper - zinc smeltery were investigated
摘要對某銅鋅冶煉廠周邊土壤微生物區系和土壤抗性細菌數進行了實驗分析。High performance ion exchange chromatography was applied in studying qualitatively and quantitatively of bacteria, which was shown as follows : firstly, physio - biochemical characteristics of bacteria was investigated by ion exchange chromatography. for the first time spores and nutrient of bacillus pumilus had been separated successfully by chromatography. chromatographial behaviors of bacteria at different cultivating environment and different growth phase were also studied
本文利用高效液相離子交換色譜系統研究細菌學，探討了該方法在細菌定性、定量方面的應用，主要包括三個方面：首先，利用離子交換色譜系統表徵細菌生理、生態方面的變化，首次成功地在色譜上區分了短小芽孢桿菌的芽孢及營養體；考察了不同的培養環境對細菌色譜行為的影響及不同生長階段的細菌的色譜行為。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。分享友人