細菌質粒 的英文怎麼說

中文拼音 [xìjūnzhílì]
細菌質粒 英文
bacterial plasmid
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Combining with practice of china ' s uranium ore heap leac hi ng, this paper proposes main ways and suitable technologies in the fields of emp hasizing feasib ility research, adop - ting strengthened technologies, improving equipment le vel, op timizing control technological factors and developing application range and so o n, which include adopting acid - curring and ferric sulphate - tric kle leaching process, bacteria heap leaching, countercurrent heap leaching, sele cting advanced material of heap bottom, developing large mechanized heap constru ction equipments and methods, popularizing drip i rrigation distributing solution, optimizing heap leaching process parameters, as we ll as developing recovery equipments suited to heap leaching, etc, in order to i n crease leaching rate, reduce heap leaching period and achieve more economic bene fits

    結合我國鈾礦堆浸實際，在重視堆浸可行性研究、採用強化堆浸技術、改進堆浸裝備水平、嚴格工藝條件及拓寬堆浸的應用范圍等方面提出一些有效途徑及適用技術，包括採用拌酸熟化-高鐵淋濾浸出、細菌堆浸、制粒堆浸、逆流堆浸，選用優質底墊層材料，研製大型機械化築堆方法和設備，推廣滴灌式布液，優化堆浸工藝參數，以及開發堆浸相配套的回收設備等，以提高浸出率、縮短堆浸周期，獲得更大的經濟效益。
  3. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  4. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論：截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達，並檢測劍其表達產物的抗原特性。
  5. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  6. The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid

    將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。
  7. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  8. A conceptual approach including measurements of materials at rest ( step 1 ), measurements using a large rotating drum ( step 2 ) or a particle - flec ( step 2 ) and measurements at a workplace ( step 4 ) has been used to characterize the release of microbial components ( bacteria, fungi, actinomycetes, endotoxin or enzymes ) and particles from straw, wood chips or fungal cultures of different ages on gypsum boards

    一套整體概念性的方法，包括物質在靜止時（步驟一） 、使用大轉動滾筒時（步驟二）或微粒逸散完時（步驟二） ，和工作場所（步驟四）進行量測，以描述由麥稈、木頭碎片或不同年份的石膏板上的真菌菌落所釋放的微生物組成（細菌、真菌、放線菌、內毒素或酵素）和微粒特性。
  9. The nitrobenzene degradative plasmid in pseudomonas xn - 1 is discussed in this paper, based on the characterization of nitrobenzene degradation by strain pseudomonas xn - 1, which attempts to do some prepared work for the research in molecule level of nitrobenzene degradation by this strain

    本論文在對菌株pseudomonasxn - 1降解硝基苯的特性及降解條件優化的基礎上，研究了菌株細胞內所含有的降解性質粒，希望能為今後對這株菌在分子水平上降解硝基苯的特性研究打下一些基礎。
  10. Gfral was high expressed in e. coli after iptg induction. by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained. based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family

    2 . gdnf的表達及其對pc12基因工程細胞的影響用本教研室已構建好的表達質粒pet一gdnf轉化大腸桿菌bl21 （ de3 ） ，經lmmipto誘導gdnf表達，並在niz氣nta柱上進行純化，稀釋復性后，純度達90 %以上。
  11. In the sky, if water droplets freeze, they must need something to stick to. dust is the material that is the easiest found in the atmosphere. smog, or even bacteria, can act as the freezing nuclei too

    在天空中，水點需要黏附在一些物質才能凝固，大氣里最容易找到的應該是塵埃了，不過煙霧甚至細菌也可以作為所需的凝結粒子呢
  12. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  13. Distribution of bacterial plasmids in taiyuan segment of fenhe river

    汾河太原段水體細菌質粒的分佈研究
  14. A few mutants were found on the plate containing nitrobenzene selective medium. their colony appearances have some difference from the wild strain. some evidences show that their growth characteristics on the selective medium are related to the plasmids harbored in their cells

    對菌株的自發突變株的研究表明，這些菌株在硝基苯選擇培養基上的生長特性是與它們細胞中所含質粒的特性相關的。
  15. So some methods suitable to large plasmid extraction, including lysis by sds and a method from a literature, were used to try to extract the large plasmid from the strain cell. the lysis reactions in these two methods are gentle, so the large plasmid cannot be injured in the lysis process, opposite to lysis by alkali. it would be helpful to keep the integrality of the large plasmid during the extraction

    因此我們採用了適合於大質粒提取的sds法，和文獻中應用於硝基苯降解性質粒的提取方法，來嘗試對菌株細胞進行質粒提取，這兩種方法裂解反應溫和，不會像堿裂解法那樣，在裂解過程中損壞質粒，可以實現質粒提取的完整性。
  16. In this paper, based on the experi ment and research data of the injected water quality in lunnan oil field in many years, the actual situation of the injected water in lunnan oil field is analyzed and discussed and the chemical indices ( content of the iron, dissolved oxygen, sulfide, carbon dioxide and oil and corrosion rote ), the bacterial index, the phys ical indices ( content and particle diameter of the suspended sub stance ) and the compatibility of injected water with formation water, etc. are evaluated one by one

    文章立足於多年來輪南油田注入水水質的實驗研究數據，對輪南油田注入水現狀進行分析討論；對化學指標（鐵含量、溶解氧、硫化物含量、二氧化碳含量、含油量、腐蝕率） 、細菌指標、物理指標（懸浮物含量、懸浮物粒徑）和注入水與地層水配伍性等進行逐一評定。
  17. To determine the plasmid function, the experiment is done to cure the plasmid from the cell, which will cause some change on the strain physiology. the result show that after curing the plasmid, the strain cell cannot grow on the selective medium containing nitrobenzene

    為了考察質粒的功能，進行了質粒消除實驗，結果表明，質粒消除后的菌株細胞難以在硝基苯選擇培養基上生長，表明這個質粒是與硝基苯的降解代謝過程有關的。
  18. Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa

    將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。
  19. The plasmid extraction from wild strains needs an appropriate method. first, lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1. but the expected result had not been got, and a lot of cracked plasmid dna fragments were got only

    對於野生菌株的質粒提取，需要選擇合適的提取方法，我們首先採用了細菌細胞質粒提取常用的堿裂解法，但是未取得理想的效果，只得到了許多破碎的質粒dna片段。
  20. These results are very important for us to further understand the genetic background, biological characteristics, evolutionary rule and the anti - schistosomajaponicum mechanism of microtus fortis at the molecular levels. the specific base changes of the dna fragme nt between the two subspecies are probably correlative with the animal immigration, survival conditions, and species evolution. the cdna library of microtus fortis bone marrow cells was transferred in situ to nylon membrane, which was divided into eight equals ( ga ~ - gh )

    利用已經建立的東方田鼠骨髓細胞質粒cdna文庫，將cdna文庫轉化菌落印跡至尼龍膜，將膜均分成8份（ ga gh ） ，制備基因池，分別培養、提取基因池質粒dna ，通過lipofect - 2000脂質體轉染技術，將基因池質粒dna導入hek293細胞， 48h后收集轉染細胞上清液，即條件培養基。
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