終止子 的英文怎麼說

中文拼音 [zhōngzhǐzi]
終止子 英文
teminator
  • 終 : Ⅰ名詞1 （最後; 末了） end; ending; finish 2 （指人死） death; end 3 （姓氏） a surname Ⅱ形容詞（...
  • 止 : Ⅰ動詞1. （停止; 攔阻） stop; cut out 2. （截止） close; end Ⅱ副詞（僅; 只） only; just Ⅲ名詞（姓氏） a surname
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 終止 : 1 （結束） stop; end; suspend 2 （停止） termination; annulment; abrogation 3 [音樂] cadence; 終...
  1. 27 unniraman s, prakash r, nagaraja v. alternate paradigm for intrinsic transcription termination in eubacteria

    我們還發現了8對雙向不依賴終止子bi - directional terminators 。
  2. Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

    本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。
  3. Effect of mifepristone in combination with misoprostol in terminating early pregnancy in women with myoma

    米非司酮配伍米索前列醇終止子宮肌瘤患者早孕的探討
  4. 7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp

    7 、首次以fam熒光素標記探針5 』端作為發光基團，以tarma標記探針3 』端為淬滅基團，以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a ）為檢狽目標，設計、篩選出特異性引物和探針，優化實驗參數，建立了轉基因植物通用性熒光pcr定性檢測方法體系。
  5. J mol biol., 1992, 224 : 53 - 63. 26 abe h, aiba h. differential contributions of two elements of rho - independent terminator to transcription termination and mrna stabilization. biochimie, 1996, 78 : 1035 - 1042

    通過計算，我們預測到266個不依賴終止子，其中包括232個蛋白編碼基因， 12個trna基因和3個rrna基因，約17 %不依賴終止子位於操縱子的末端。
  6. 2. in order to express chs in plant, we constructed plant expression vector p1301b q c. first added camv 35s promoter and nos terminator to chs by one median clone

    （ 2 ）為了能在在植物中表達（ chs ，通過一步的中間克隆，將chs連上camv35s啟動子和nos終止子。
  7. 25 reynolds r, chamberlin m j. parameters affecting transcription termination by escherichia coli rna. ii. construction and analysis of hybrid terminators

    研究synechococcus sp . wh8102的不依賴終止子可促進對該菌基因調控網路的理解。
  8. Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay

    由於vgb基因啟動子不能在枯草桿菌中啟動表達，因此，根據已發表的果聚糖蔗糖酶基因（ sacb ）序列設計引物，從枯草桿菌wb600總dna中擴增出該基因的啟動子片段，然後將其與vgb基因編碼區及終止子序列相連，成功地組建了sacvgb融合基因。
  9. Another detection method, dot blotting, was built also based on 35s promoter and nos terminator. the two genes were labeled by digoxin to done as probe used in dot blotting

    並將35s啟動子和nos終止子用地高辛標記后制備成基因探針，建立了轉基因檢測的分子雜交技術?點雜交。
  10. The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work

    高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。
  11. A new method for intrinsic terminator prediction based on rnall, an rna local secondary structure prediction algorithm developed recently, and two u - tail score schemas are developed. by optimizing three parameters thermodynamic energy of rna hairpin structure, u - tail t weight, and u - tail hybridization energy, the method can recognize 92. 25 of known terminators while rejecting 98. 48 of predicted rna local secondary structures in coding regions negative control as false intrinsic terminators in e. coli. this method was applied to scan the genome of synechococcus sp

    在過去二十年中，不少研究者已開始研究如何用計算方法來預測轉錄終止信號，如brendel和trifonov的雙核苷酸分佈矩陣法dinucleotide distribution matrix carafa等的統計方法transterm和rnamotif法等，這些方法都從不同方面考慮了rna二級結構和u -尾部的特徵，而gester的預測模型則設定rna二級發夾結構是不依賴終止子的唯一因素。
  12. The blotting detection showed : the pcr detection on 35s and nos is correct, while when the gms content decrease the detection signal weakened. so this method was not fit to be applied in market detection

    雜交試驗證明pcr擴增的35s啟動子和nos終止子為特異性擴增，當材料轉基因含量降低時，檢測信號則明顯降低。
  13. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  14. 5. in this study, we have cloned camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryia ( b ) genes used as positive collate

    5 、克隆了camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1ao ）的陽性質粒作為轉基因植物檢測的陽性對照。
  15. Then, 5. 5kb thrombiotin gene was amplified with the same technique from the genome of a baby ' s blood, which included the begining part of intronl to the teminator. in addition, 6. 0kb and 1. 8kb homlogous arms were also amplified from a cow with high yield. the 6. 0kb homologous arm contains the promotor, extron 1, extron2, extron3 and intron 1, intron2 and part of the intron3 fragment, while the 1. 8kb homologous right arms contains exon13, exon14 and part of intron 13, the whole intron14 and part intron 14 of asl - casein gene of bovine

    通過長片段pcr從高產奶牛的基因組中獲得了打靶所需的長、短同源臂序列，長度分別為6 . 0kb和1 . 8kb ，位於s1 -酪蛋白基因的5上游區到第三內含子和十二到十四內含子；從綿羊全血基因組克隆得到了綿羊的-酪蛋白基因啟動子區到第二內含子區4 . 1kb的5調控序列；利用同對引物克隆得到了水牛的同基因序列；從廣西當地一嬰兒臍血基因組中通過獲得了人血小板生成素基因，位於第1內含子到終止子後部分的序列，長達5 . 5kb 。
  16. Ln our experiment, a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos, the fl sequence and kozak sequence for improving the genetic statement in the level of translation, were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process, and the products were linked into pbil21

    本實驗中人工合成了美洲擬鰈抗凍蛋白基因，並在美洲擬鰈抗凍蛋白基因兩側添加了許多調控序列，如具有加倍增強子的35s啟動子和nos終止子，它們可以促進抗凍蛋白基因的轉錄，及可在翻譯水平上提高基因表達的序列和kozak序列，從而構建了抗凍蛋白基因的高效表達盒。
  17. A new e. coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1, bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed. 2, a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter. 3, another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19

    進一步以紅移且熒光強度提高21倍的gfpmut3為報告基因，構建了大腸桿菌啟動子探針載體phn1005 ，該載體上gfpmut3結構基因5 』端的bamhi位點可用來克隆具有啟動子活性的dna片段並定量分析插入的啟動子強度；其3 』端含rrnat1t2終止子，可允許克隆強啟動子；在bamhi上游同樣插入rrnat1t2終止子以防止載體puc19上的啟動子的轉錄通讀； gfpmut3結構基因上游還插入一段內含子序列使正反六種讀碼框的翻譯均可被終止，可保證gfpmut3以正確的讀碼框翻譯。
  18. Intrinsic terminator prediction and its application in synechococcus sp

    不依賴r終止子的預測及其在synechococcus sp . wh8102中的應用
  19. The results showed that the sequence of two strains basically coincided but differenc

    因此， biob與biof基因間終止子序列的刪除，不影響基因的表達。
  20. 4 yarnell w s, roberts j w. mechanism of intrinsic transcription termination and antitermination. science, 1999, 284 : 611 - 615. 5 gusarov i, nudler e. the mechanism of intrinsic transcription termination

    本論文報道了一種不依賴終止子預測的新方法- rnall法，它建立於我們最近開發的rna局部二級結構和以前發表的兩種u -尾部分數計算方法。
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