組成酵素 的英文怎麼說

中文拼音 [zǔchéngjiàosù]
組成酵素 英文
constructive enzyme
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 成 : Ⅰ動詞1 （完成; 成功） accomplish; succeed 2 （成為; 變為） become; turn into 3 （成全） help comp...
  • 酵 : 動詞（發酵） ferment; leaven
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 組成 : form; make up; compose; formation; composition; configuration; make-up; compo
  • 酵素 : biocatalyst
  1. Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da

    採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱，通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰，這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的，通過tricine - sds - page檢測，只能檢測到一條分子量相對較大的細菌素，分子量在8 ， 570da左右。
  2. Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins

    將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。
  3. Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected

    搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。
  4. In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated

    將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。
  5. A conceptual approach including measurements of materials at rest ( step 1 ), measurements using a large rotating drum ( step 2 ) or a particle - flec ( step 2 ) and measurements at a workplace ( step 4 ) has been used to characterize the release of microbial components ( bacteria, fungi, actinomycetes, endotoxin or enzymes ) and particles from straw, wood chips or fungal cultures of different ages on gypsum boards

    一套整體概念性的方法，包括物質在靜止時（步驟一） 、使用大轉動滾筒時（步驟二）或微粒逸散完時（步驟二） ，和工作場所（步驟四）進行量測，以描述由麥稈、木頭碎片或不同年份的石膏板上的真菌菌落所釋放的微生物組成（細菌、真菌、放線菌、內毒素或酵素）和微粒特性。
  6. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  7. To explain the nature of food as a medium for chemical reactions and the effects of electrolytes, non - electrolytes, ph and dispersed phases on the structures and reactivities of food components ; to examine in detail the mechanisms of non - enzymic browning, food additive - food component reactions, and methods of control

    介紹食物的化學反應、電解質與非電解質的效果、食物元素的酸堿值發散階段及組織與反應、非酵素褐化的結構、食品添加物成分與反應、與控制方法等。
  8. These enzymes are significant as a digestive aid to larval fish, improving survival and enhancing growth. sick and stressed fish are also beneficiaries of these enzymes. genchem polytase is our top of the line product, genchem polytase is a compound of several enzymes, rhizoma coptidis and cortex phellodendri extracts which can speedily and safely dissolve left - over feed, thus solving the problem of sediment formation and the stress caused by nitrous or ammonia

    維生消化素也是消化整腸酵素，經由多種酵素及黃蓮，黃柏抽出物的綜合體，因為是多種酵素的組合所以能迅速分解殘餌，完全解決污泥的產生，免除亞硝酸及阿摩尼亞所造成的緊迫甚至泛池，更因為神奇的酵素作用能預防及免除使擾亂幼蝦正常代謝的生化與營養變化物質，而獲得幫助蝦苗消化酵素分化的正常機能，提高飼料效率，增加存活率防止疾病。
  9. Although yeast cannot digest cellulose or lignin, the molecules that form a plant ' s skeleton, some bacteria and other species of fungi are able to do the job

    雖然那些組成植物莖干纖維素和木質素的分子不能為酵母所消化，但是某些細菌和其他種類的真菌可以完成這項任務。
  10. Zymosn in the kangsheng multi - nutriment is consist of gluconsan and mannan. zymosan is the most active glucosan in life - bodies, which has the function of human immunity, anti - cancer, anti - inflammation, anti - radiation and reduce the cholesterol

    康聖天元多價營養素中酵母多糖由葡聚糖和甘露聚糖組成，酵母葡聚糖是生物活性最強的葡聚湯，具有人體免疫、抗腫瘤、抗炎、抗輻射和降低膽固醇的作用。
  11. The elements affecting the formation of high alcohols include raw material, composition of malt liquid, sorts and quantity of yeast, fermentation condition, concentration of oxygen dissolution, fermentation degree and storage period

    影響高級醇形成的主要因素包括原料、麥汁的組成、酵母菌種類和接種量、發酵條件、溶解氧濃度、發酵度和貯存期。
  12. Introduced beer brewing in the process the diacetyl formationmech anism and the influence factor and the control method, from theyeast mold mushroom spawn, the wheat juice component, pass craft and so on oxygen quantity carries on the adjustment, may in the ac tivecontrol beer the diacetyl content, the improvement beer maturity

    摘要介紹了啤酒釀造過程中雙乙酰的形成機理及影響因素和控制方法，從酵母菌種、麥汁組分、通氧量等工藝進行調節，可以有效控制啤酒中雙乙酰的含量，改善啤酒的成熟度。
  13. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  14. The deletion mutants were proved to be genetically stable, and thus might be promising strains in industrial production of avermectins b. although s. avermitilis produces eight related components of avermectins, only two components, bla and bib, are available for the medical, veterinary and agricultural fields

    該缺失突變是在染色體上通過同源雙交換完成的，不會發生進一步的重組，因此突變株性狀穩定，在工業生產上具有應用價值。阿維菌素的天然發酵產物共有八個組分，其中只有b1組分的殺蟲活性最高，被作為殺蟲劑在農業和畜牧業中使用。
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