組成性表達 的英文怎麼說

中文拼音 [zǔchéngxìngbiǎodá]
組成性表達 英文
constructive expression
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 成 : Ⅰ動詞1 （完成; 成功） accomplish; succeed 2 （成為; 變為） become; turn into 3 （成全） help comp...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 表達 : deliver; express; show; voice; convey; communicate
  1. The changes of the expression of gfp in biu - 87 cell that induced by the aconitine and hab toxins, gtx were detected with fluorescence microscope, and quantitatively measured with image - pro plus software

    經誘導，抗性細胞發出較強的綠色熒光，表明重組質粒pegfp - c - fos在biu - 87細胞中成功表達。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Mouse homeobox gene pem was newly isolated in 1990 ' s. its expression pattern during the murine ontogeny and in the adult tissues is unique, namely, in a stage and spatial specific manner

    Pem基因在小鼠發育過程及成體部分組織中呈時間和空間特異性表達，並在一系列腫瘤細胞株中持續表達。
  4. These results indicated that the cloned osg6b " had a constitutive characteristics but not anther specificity, and the transgene was segregated in a mendelian fashion in the tl generation

    研究表明，所克隆的osg6b 』不具有花藥特異性，而具有組成型表達的特徵。第二部分：從大腸桿菌e
  5. Driven by the cauliflower mosaic virus ( camv ) 35s promoter, the er - shsp over - expressed constitutively. the growth and the phenotype of transgenic plants can be used for researching the function of er - shsp in improving tomato ' s cold resistance and the er - shsp chaperones function in vivo. after degested by kpnl and xbal enzymes from the pbs - er plasmid, the gene er - shsp - lehsp21. 3 was inserted into the prokii vector to construct an eukaryotic expressing vector

    利用基因工程方法，將內質網小分子熱激蛋白基因（ endoplasmicreticulum - locatedsmallheatshockproteingene ， er - shspgene ） - lehsp21 . 3導入到番茄體內，使之在植物體中組成性表達（ constitutiveexpression ）內質網小分子熱激蛋白（ er - shsp ） ，觀察轉基因番茄在低溫條件下的生長和表型反映，研究er - shsp在提高植物耐寒性中的作用，同時為體內研究er - shsp的分子伴侶機制提供依據。
  6. It can be boiled down to combinatorial optimization problem in mathematics. on the basis of summarizing the complexity and structural features of hmb and rules of its design and manufacture and analyzing the spatial relationship in 3d layout of hmb, the expressions of relevant variants are put forward using the object - oriented approach

    在全面總結液壓集成塊設計問題的復雜性特點，以及集成塊類零部件的結構特徵和設計、製造信息組成規律的基礎上，本文深入分析了集成塊立體布局的空間關系，用面向對象方法定義了與該問題有關的特徵變量的示性表達式，給出優化目標和約束條件，進而確立了集成塊設計問題的數學優化模型。
  7. So, we can concluded that over - expressed constitutively er - shsp can improved the tomato ' s cold - resistance indeed

    由此我們可以確定， er shsp的組成性表達確實提高了番茄的耐寒性。
  8. Results the study had revealed that not only the bone tubular and the degree of mineralization, but also the activity expression of alkaline phosphatase of osteoblasts in fluoride group were better than the control group

    結果研究發現氟組成骨細胞不僅其礦化結節和礦化程度，而且其堿性磷酸酶活性表達都要早於對照組。
  9. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  10. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  11. The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction

    目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。
  12. These results showed that hsp70 is highly conserved in shrimps. semi - quantitative rt - pcr was developed to study the hsp70 transcription pattern in fenneropenaeus chinensis. the transcription of hsp70 was detected in the major tissues of hepatopancreas, muscles, eyestalks under both normal and heat shocked conditions. the mrna level was increased after the animals were heat shocked in the 3 tissues

    為了研究中國對蝦hsp70的轉錄表達，發展了半定量rt - pcr方法，對hsp70轉錄表達分析顯示， hsp70mrna在中國對蝦正常肝胰腺、肌肉、眼柄組織存在組成性表達；在受到整體熱休克后的對蝦肌肉、肝胰腺、眼柄組織hsp70轉錄水平增加。
  13. In this dissertation, influences of various factors on plant regeneration and agrobacterium - mediated transformation of tall fescue embryogenic calli were systematically studied, and thereafter, stress tolerance - related cbf1 gene guided by constituent promoter camv 35s was incorporated into genome of this grass to obtain transgenic plants with increased stress tolerances

    本論文在對高羊茅胚性愈傷組織植株再生與農桿菌介導遺傳轉化的多種影響因素進行系統研究的基礎上，將組成型表達啟動子camv35s引導的耐逆相關cbf1基因導入該草種的基因組，獲得耐逆性增強的轉基因植株。
  14. Constitutive expression of class ii mhc is restricted to " professional " antigen - presenting cells but can be induced on various tissues by gamma interferon

    Mhc -類的組成型表達僅限於「專職性」抗原提呈細胞，但細胞因子如ifn -可引起誘導型類分子表達。
  15. Analysis of acbadh expression in leaves showed that the expression levels of acbadh increased about 10 - fold after plants were treated with nacl and aba for 48 hours when compared to control plants respectively

    Northernblotting雜交結果表明， badh具有組成型表達的特性。 100umaba和400mmnacl處理48小時后， badh的表達增加10倍左右。
  16. A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127. a group of constitutive - expression fusions with different fluorescent strength were obtained

    將7653r的總dna經sau3ai部分酶解並克隆到phn127上，獲得的融合子庫，從中篩選到gfp組成型表達的具有調控活性的dna片段。
  17. However, so far only the results obtained by whitelaw et al ( 2002 ) concerning leetrl gene were partly consistent with the negative model and partly against it. obviously, the action models for these ethylene receptors are far from being clearly understood

    目前已經從模式植物番茄中分離了6個受體基因（ leetr1 6 ） ，對它們的基因和蛋白結構特徵、表達特性等已經有較多的研究，但是對組成型表達受體基因在乙烯受體系統中的功能研究較少。
  18. In this study we constructed two promoters ( dgp1 and sigp1 ) and analyzed their function in transgenic tobacc os. we combined the dehydration responsive element ( dre ) with guard cell specific element ( gcse ) to construct a novel drought - induced guard cell - specific promoter - dgp1

    受乾旱誘導的保衛細胞特異性表達啟動子（ dgp1 ， droughtinducedguardcellspecificpromoter ）的構建：利用pcr的方法，擴增出乾旱應答元件和保衛細胞特異性元件，然後組裝而成。
  19. The genomics dna of the transformants was extracted and assayed by pcr with nptii primer camv35 / cp primer and the results indicated that the chloroplast shsp gene has been integrated into the genomics of the tomato. then the transgenic tomato were exposed to low temperature ( in winter, on natural condition, the top temperature was 15 ? and the lowest temperature was 5 and a set of physiology parameters were measured after 6 weeks. the results were shown as follows : 1 ) effect on growth height of the transgenic tomato and the control plants after 6 weeks at low temperature showed that the transformants had been grown faster than the control. in addition, the leaves of the control plants appeared to be much reder than the transgenic tomato, and the change were obvious followed by far from the treated time at low temperature, which suggested that the constituently expression of the chloroplast shsp had some protective fountions to the tomato at low temperature

    提取轉基因番茄基因組dna ，分別以npt和35s cp引物對其進行pcr分析，結果表明葉綠體shsp基因已整合進番茄基因組中；對轉基因番茄進行低溫處理（冬季，自然條件下（無加熱的溫室） ，白天最高溫度15 ，夜間最低溫度5 ） ，生長6周后，檢測轉基因番茄的系列生理指標，主要結果如下： 1 ）生長勢：測量轉基因番茄與對照（未轉基因番茄）的株高，結果顯示轉基因植株生長明顯快于對照，且從外觀上看到對照葉片發紅程度遠大於轉基因植株，隨著低溫時間延長，對比更加明顯，說明葉綠體shsp的組成性表達在低溫下對番茄具有一定的保護作用。
  20. It sets up the improved spring - mass model, derives the formulation for dynamic system of modeling and simulation with given composition and expression in internal and external cloth forces, describes the simulation algorithm

    摘要對彈簧質點模型進行了改進，給出模型方程中質點所受內力和外力的組成與表達式以及動態系統的推導和求解過程，並且描述了模擬柔性受風飄動的具體實現演算法。
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