組織培養條件 的英文怎麼說

中文拼音 [zǔzhīpéiyǎngtiáojiàn]
組織培養條件 英文
conditions of tissue culture
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 織 : 動詞（編織） knit; weave
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 條 : Ⅰ名詞1 （細長的樹枝） twig 2 （條子） slip; strip 3 （分項目的） item; article 4 （層次; 秩序; 條...
  • 件 : Ⅰ量詞（用於個體事物） piece; article; item Ⅱ名詞1. （指可以一一計算的事物） 2. （文件） letter; correspondence; paper; document
  • 組織 : 1 （組織系統） organization; organized system 2 （組成） organize; form 3 [紡織] weave 4 [醫學] [...
  • 條件 : 1. （客觀的因素） condition; term; factor 2. （提出的要求） requirement; prerequisite; qualification
  1. With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

    （ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。
  2. Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency

    多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。
  3. A homolog of ga sbeta - hydroxylase gene expression pattern was examined. hybridization signals of this gene could be detected at day 5 and however, they appear again after day 10 when explants cultured with hormones. it has the similar pattern when the explants cultured without hormones or uncultured

    風信子ga羥基化酶類似基回在外植體表達，在含有外源撒素條件下培養的前5天受到激素的抑制而幾乎不表達，第10天開始在花分生組織中表達。
  4. Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth

    建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。
  5. The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6

    方法無菌條件下，從6月齡紐西蘭白兔的膝關節囊內剪取滑膜組織，採用組織塊培養法和酶消化法分離滑膜細胞。
  6. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  7. The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body

    小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑，其制備、傳代及冷凍保存均有不同的研究報道，飼養層的質量受許多因素的影響，如動物的品種、培養液、所傳代數及實驗條件等，關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少，故本實驗以昆明小白鼠為實驗動物，研究其胚體培養過程中細胞的形態學、組織學等方面的變化，以期為本實驗室建立飼養層細胞庫提供理論依據，同時探討心肌細胞和成纖維細胞層共培養的可行性，以期為心臟生物學特性的體外研究奠定基礎。
  8. In this scientific dissertation, some medicinal plant tissues were chosen as the materials for separation of antimicrobial endophytes, a11, one of produced wide - spectrum antibacterials endophytes isolated from aloe, was indentified by physiological and biochemical techniques, and the antimicroorganism substances were purified. the results were as follows. 1

    實驗結果如下： 1 、採用組織表面消毒的方法從銀杏、蘆薈等十多種植物組織中分離出了115株植物內生菌，其中細菌60株，真菌55株，這些真菌在28恆溫條件培養大多都不產孢。
  9. Many researchers have conducted experimellts about it, but not succeeded. culture media were compared to find the best medium of gingkgo culture for controlling callus browning by different sugars, antioxidants and sorbents. the result showed that the medium with ms + zt 1. 0mg / l + naa 1. 0mg / l + sucrose 50g / l + agar 8g / l + ac 2g / l was the best medium, at l5 days subculturing intervals

    銀杏組織培養過程中，尤其是在愈傷組織的繼代培養中，褐變現象特別嚴重，曾有不少的人做過這方面的研究，但都沒有成功，而本研究通過對不同糖類物質、抗氧化劑、吸附劑以及不同的培養基對褐變的影響和控制效果，探索出有效控制褐化現象發生的最佳培養條件，試驗結果表明： ms + zt1 . 0mg / l + naa1 . 0mg / l +蔗糖50g / l +瓊脂8g / l + ac2g / l培養基上的繼代效果最好，繼代時間最好在15d左右。
  10. This thesis clarifies the development course of household appliance enterprises of china ( haec ), and summarizes the characteristic and evolution tendency. it elaborates the qualitative and quantitative method to expound the principle and index system for evaluating marketing management situation ( mms ) of those haec. furthermore, it employs a typical instance analysis to the evaluating method so as to deduce the actual mms of haec

    在營銷管理對策的探討上，本文對我國家電企業的營銷管理環境進行整合以優化家電業產業組織結構，提出了通過國家改進營銷管理人才的培養模式和激勵機制、完善我國家電企業的產權治理機制，為企業的營銷管理創造良好的外部條件。
  11. Pre - differentiation and proper partial desiccation of calli before transferred to regeneration medium was found to apparently improve the frequency and quality of plant differentiation. with our optimized culture condition and treatment style, induction frequency of pei ' ai64s and 9311 can be reached 62. 45 % and 85. 30 % respectively and after 3 months of subculture calli can remain high - quality embryogenic state, when high - quality embryogenic calli after two times of subculture were used as acceptor, callus differentiation frequency can arrive at 85. 5 % and 87. 7 % respectively

    採用我們優化后的培養條件與處理方式，培矮64s和9311愈傷組織的誘導頻率分別可達到62 . 45和85 . 30 ；繼代培養時間達三個月左右仍能保持較好的胚性生長狀態；對于繼代兩次胚性生長狀態良好的愈傷組織分化頻率分別可達到85 . 5和87 . 7 。
  12. The authors suggested that the government should create favorable conditions and atmosphere for developing peasant sport, speed up the construction and perfection of supporting small towns and sport organization networks for rural areas, cultivate sports backbones, utilize the sports capital of local schools, enterprises and institutions, and guide peasants to utilize local characteristics and resources to develop featured sports activities

    建議政府要為發展農民體育創造良好的條件和氛圍；加快建立和完善依託小城鎮，面向農村的體育組織網路，培養體育骨幹；利用當地學校和企事業單位的體育資源；引導農民利用地方特點和資源開展有特色的體育活動。
  13. At the same time, the count of neurons with axons, the length of axons, and the ratio of neurons in visual field under microscope in each group were analyzed

    將脊髓神經組織細胞懸液置於不同濃度的神經幹細胞條件培養液中培養。
  14. There are a lot of shortcomings of traditional tissue culture conditions, which have been improved through some ways such as culture medium, container, environmental conditions ( illumination, gas )

    摘要植物組織培養技術具有巨大的應用價值，傳統組織培養存在許多缺點，許多學者從培養基、培養容器、環境條件（光照、氣體等）進行了研究和改善，取得了一定的成效。
  15. Culture conditions for callus induction and anthocyanin accumulation of celosia cristata " phumosa " were studied in this paper

    本文研究了穗冠花celosiacristata （ phumosa ）愈傷組織誘導和影響愈傷組織生長及花色素苷積累的培養條件。
  16. Effects of different culture conditions on callus salidroside contents of rhodiola sachalinensis

    不同培養條件對高山紅景天愈傷組織紅景天甙含量的影響
  17. In inducing callus process, the suitable illumination conditions were the former period with darkness culture and the later period with light culture. in inducing dedifferentiation and redifferentiation process, the temperature of 24 - 26 was suitable

    在愈傷組織誘導時，光照條件以前期暗培養、後期光培養為宜，在誘導脫分化和再分化時，培養條件以溫度24 - 26為宜。
  18. The results showed that : when cultured in the medium of m199, supplemented with 20 % bovine serum containing a moderate amount of antibiotics, the incubtion ph 7. 2 - 7. 4, the culture temperature 28. the primary culture cells formed the dense single wall within three weeks, the generation time of the subculture cells was 5 - 6 days, most cultured cells were fibroblastic - like cells with few epithelial - like cells

    研究初步表明：在m199培養基加入20小牛血清（常規量雙抗） ， ph在7 . 2 - 7 . 4之間， 28的培養條件，四倍體鯽鯉魚腎組織原代培養三周左右即可形成緻密單層，傳代細胞為5 - 6天左右傳一次。培養細胞以成纖維樣細胞為主，有少數上皮樣細胞。
  19. The constuction of plant expression vector, tissue culture of cabbage and gene transformation conditions were studied to obtain ubic transformants. ubic gene was obtained from the escherichia coli. by pcr amplication and was ligated to puc118 vector

    為獲得轉ubic基因甘藍，對植物基因工程載體的構建、甘藍的組織培養以及基因轉化條件進行了研究。通過pcr方法從大腸桿菌基因組中擴增得到了ubic基因，擴增產物克隆到puc118載體，轉化大腸桿菌jm109 。
  20. Traditionally autologous chondrocytes implantation is applied at the damage site, which has many problems such as the losing of its primary characteristics

    胚胎幹細胞具有自我更新和多分化潛能的特性，在一定培養條件下經適當刺激可誘導分化成軟骨組織。
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