組織提取液 的英文怎麼說

中文拼音 [zhī]
組織提取液 英文
tissue extract
  • : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
  • : 動詞(編織) knit; weave
  • : 提動詞(垂手拿著) carry (in one's hand with the arm down)
  • : Ⅰ動詞1 (拿到身邊) take; get; fetch 2 (得到; 招致) aim at; seek 3 (採取; 選取) adopt; assume...
  • : 名詞(液體) liquid; fluid; juice
  • 組織 : 1 (組織系統) organization; organized system 2 (組成) organize; form 3 [紡織] weave 4 [醫學] [...
  • 提取 : 1. (取出) draw; pick up; collect 2. (提煉) extract; abstract; recover
  1. Extract the bursin from the bursa fabricius of chichens and ducks through the ultrafilter with loooda filter. ( the bursin of chickens - cbs. the bursin of ducks - dbs ) determine the major constitution by high - performance liquid chromatography ( hplc ) and thin - layer chromatography ( tlc ). it is proved that there is bursin ( bs ) in two extractions. caculate the quantitation of bursin in the extraction of the bursa fabricius of chickens and ducks. it can be found through tlc that the quantitation of cbs in the extraction of the bursa fabricius of chickens is 1. 436mg / ml and the quantitation of dbs in the extraction of the bursa fabricius of ducks is 4. 035mg / ml. the result obtained through hplcis that the quantitation of cbs is 1. 199mg / ml and dbs is 3. 316mg / ml

    採用超濾法從正常雞、鴨法氏囊分子量小於1000da的物質,分別以薄層層析法和高壓相色譜法檢測其主要成分,證明兩種超濾中均有法氏囊活性肽(囊素三肽, bursin , bs )的存在,分別計算雞、鴨法氏囊物中的囊素三肽的含量。薄層層析方法求得雞法氏囊物中的bs含量為1 . 436mg / ml ,鴨法氏囊物中的bs含量為4 . 035mg / ml ;高壓相色譜的方法求得雞法氏囊物中的貼含量為1 . 199mg / ml ,鴨法氏囊物中bs含量為3 . 316mg / ml 。
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    正清然科技公司作為立志從事綠色環保事業的一員,鑒於我國目前以城市為中心的環境污染日益嚴重,並有急劇向農村蔓延之趨勢,深深感受到自己身上的責任義務和緊迫感,於2000年初了國內一流的科研開發人員,用了四年的時間傾力攻關,厚積薄發,拿出了擁有自主知識產權的「碧清」品牌清潔護理系列產品,與其他有機溶清潔產品不同的是,該品主要採用純天然植物精華為原料,對所清潔對象採去污上光除菌護理並可形成保護膜,達到濕潤滋護防老化抗黃變防靜電等作用,是目前最為理想的綠色環保清潔護理系列用品之一,該品的研製,填補了國內空白,並達到世界領先水平。
  3. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  4. To find dnaase in the earthworm the tissue extract of earthworm with different concentration reacted with rna, circular dna, linear dna for an hour at 37, then the producetion was detected by 1 % agrose gel. the tissue extract of earthworm, the tissue protein extract of earthworm and the tissue extract of earthworm without protein reacted with pbv220 - r - inf for an hour at 37, then the producetion was detected by 1 % agrose gel. 2

    雙胸蚓中dna酶的發現用不同濃度的雙胸蚓組織提取液與rna 、環狀dna及線狀dna在37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察;雙胸蚓、雙胸蚓蛋白粗及雙胸蚓去蛋白分別與pbv220 - ? inf質粒37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察。
  5. Under the same condition, the tissue extract of earthworm and the tissue protein extract of earthworm could decompose the pbv220 - r - inf completely but the tissue extract of earthworm without protein could n ' t. 2

    雙胸蚓及雙胸蚓的蛋白在37oc保溫1小時可以完全消化pbv220 p一inf質粒而雙胸蚓去蛋白對pbv220 y一wf質粒無消化作用。
  6. The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane

    雙胸蚓中dna酶的分離純化雙胸蚓經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素( de52 )柱層析、超濾膜分級分離后得到一個電泳純的dna酶。
  7. Results : ( 1 ) obtained the levels of monoamine transmitters and their metabolites in the cerebrospinal fluid ( csf ), spinal perfusate and dorsal half of the spinal cord ; ( 2 ) observed that 5 - ht, na and da existed in the dorsal half of the spinal cord, but only na could be detected in all samples of the csf and spinal perfusate, da could be detected in part of samples ; ( 3 ) the contents of the metabolites of monoamine transmitters are higher than their corresponding transmitters in the csf and spinal perfusate

    結果: ( 1 )分別測得了大鼠腦脊、脊髓灌流和脊髓背部組織提取液中單胺類遞質及其代謝產物的含量; ( 2 )在大鼠脊髓背部中同時含有5 -羥色胺、去甲腎上腺素和多巴胺,但在脊髓灌流和腦脊中只能檢測到去甲腎上腺素,部分樣品中能測到多巴胺; ( 3 )遞質代謝產物在腦脊和脊髓灌流中的含量均高於其對應的遞質。
  8. Secondly, put acetone in the abstracting liquor, put it aside for one hour and slowly centrifuge. thirdly the deposit is fully dissolved and heated for 15 minutes in 50 c water, and get crude enzyme solution through centrifuge

    分離純化將蘆薈的葉子洗凈、擦乾,加入tris - hcl緩沖進行搗碎sod ,高速離心;將用丙酮使sod沉澱、靜止,低速離心;將沉澱用蒸餾水充分溶解, 50水浴中加熱15min ,離心除去部分雜蛋白,獲得粗酶
  9. To isolate and purify dnaase in the earthworm first, the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer. then dnaase was purified by denaturing the protein with higher temperature. the following steps were ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane

    雙胸蚓中dna酶的分離純化採用蔗糖溶解雙胸蚓,並選擇性酸變性制備雙胸蚓,再經選擇性熱變性、硫酸銨分段鹽析、 deae ?纖維素( de52 )柱層析及超濾膜分級分離對雙胸蚓中dna酶進行分離純化。
  10. It is true in difficult cases, such as degraded dna or samples containing minimal amounts of genomic dna. to investigate the sequence diversity of mtdna in taiyuan han population, mtdna nt16081 - 16546 sequences were determined in 58 unrelated han chinese from taiyuan. sscp method was developed as one of screening measures which were used to examine polymorphism of mtdna, so as to supply a rapid and simple method for forensic casework

    方法隨機抽58名太原地區漢族群體無血緣關系個體的靜脈血, edta抗凝,改良的tkm法mtdna , pcr擴增、純化后,應用377序列分析儀進行直接測序分析;對毛發、指甲、骨骼等微量或嚴重降解檢材進行mtdna序列分析;採集同一屍體血、肌肉、肝、腎、心肌、不同部位的毛乾等進行同一性檢測;對20個兩代家系進行突變觀察。
  11. 2. preparation of crude cell extracts ; the cultured cells were treated with different medium containing 17 - e2 and / or progesterone of different concentration

    用不同濃度的雌激素分別處理卵巢癌的端粒酶zh h在h ,加乙醇純,去乙醇,放置一20冰箱待用。
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