結合子誘導 的英文怎麼說

中文拼音 [jiēgěziyòudǎo]
結合子誘導 英文
zygotic induction
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 誘 : 動詞1. （誘導） guide; lead; induce 2. （使用手段引人隨從自己的意願） lure; seduce; entice
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 結合 : 1 （發生密切聯系; 聯合） combine; unite; integrate; link; binding; coalition; cohesion; connectio...
  • 誘導 : guide; lead; induce; guidance; induction
  1. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %

    本研究的主要結果和結論如下：一、經抗原分子設計，在430a自動肽合成儀上合成的新抗原p3 ，經質譜儀分析證明其荷質比與理論一致；純化后，其純度大於95 ；將新抗原與klh偶連后免疫小鼠，經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。
  4. Aiming at the present design fashion of domestic bigger tunnel, simulating a bigger tunnel with catholic characteristic, confirming the parameters of traffic inducement and controlling ( tunnel length, traffic, the selection and location of detection equipments, amount of roadway indicative lamp, etc ) on the base of the analog tunnel, discussing the controlling and revulsive mode of tunnel. briefly discussing the constitution of tunnel surveillance and controlling system and the executive means of traffic controlling and inducement subsystem and network structure of tunnel controlling system. finally discussing the conformation of emulational tunnel ' s database and detailed executive program by programming the computer emulation of controlling induce subsystem

    論文圍繞「交通誘導與控制」這一中心展開，探討長大隧道交通誘導與控制設備及其控制誘導方式；結合國內外對交通流模型研究的成果，提出一套適合長大隧道交通流特點的交通流模型；並針對目前國內長大隧道的普遍設計方式，模擬一個帶有普遍性特徵的長大隧道，確定了關于交通誘導與控制方面的參數（隧道長度、交通量、檢測設備的選取和位置、車道指示燈的數目等） ；以此模擬隧道為基礎，進一步探討隧道的具體控制與誘導方式；簡要探討隧道監控系統的構成、交通控制與誘導子系統的實現方式，隧道控制系統的網路架構；最後通過編程實現控制與誘導子系統的計算機模擬，討論關于模擬隧道數據庫的構建，具體編程實現等。
  5. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  6. The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot

    4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。
  7. The levels of metallothionein synthesized in liver of the mice induced by different stress factors including trauma, starvation, zinc ( zn ) nutriment, cadmium ( cd ) poison, both zn nutriment and cd poison and different treating ways which were designed according to the time - effect and dosage - effect were detected by atomic absorb mean and sulfydryl saturation mean respectively

    用鎘飽和法及巰基顯色法兩種方法檢測小白鼠肝臟mt的量，研究不同應激因子誘導小白鼠肝臟mt合成的對應情況，結果表明： 1鎘常規毒理實驗中，鎘對小白鼠腹腔注射的半致死劑量為2 . 12 （ 2 . 67 1 . 71 ） mg / kg 。
  8. Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin. the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5. 4 ). the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5. 4 ), which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching

    第一部分：等離子點ph （ 5 . 4 ）條件下，用平衡透析法和紫外光譜，熒光光譜，共振散射光譜研究了ag （ ）與人血清白蛋白（ humanserumalbumin ，簡稱hsa ）或牛血清白蛋白（ bovineserumalbumin ，簡稱bsa ）的結合。 scatchard圖分析表明， ag （ ）在hsa或bsa中有強弱兩類結合部位，通過計算機擬合獲得結合的逐級穩定常數值。紫外掃描發現ag （ ）與hsa或bsa的結合存在滯後效應，表明ag （ ）與hsa或bsa的結合可能誘導蛋白質構象發生緩慢變化（ a - b ） ，測得並討論了這一構象變化的速度常數和活化參數。
  9. The latest research progress in supramolecular fluorescence / phosphorescence sensors and switches used for metal cation recognition is summarized. two types of systems were reviewed, photo - induced electron transfer and monomer - excimer systems

    本文總結了近年來用於金屬離子識別的超分子熒光磷光傳感器的研究工作進展，著重介紹了光誘導電子轉移及單體激基締合物體系在金屬離子識別中的應用。
  10. Molecular tissue engineering should be a subject that combine molecular biology and tissue engineering, to study cells, tissue - inducing factors, biomaterials and the subtle relationships between them at molecular level and, finally develop better biological substitutes which would restore, maintain, or improve tissue function

    分子組織工程學是將分子生物學與組織工程學有機結合，運用分子生物學的理論和技術，從分子水平研究細胞、組織誘導因子、生物材料及其相互作用的關系，為研製能更有效地恢復、維持或改善病損組織或器官功能的生物替代物奠定基礎。
  11. Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag

    目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。
  12. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  13. The results were as follows : ( l ) the pre - incubation of pmr induced the exocytosis of the cortical granules ( cgs ) in the oocytes and reduced the quantity of the microvillus on the surface of oocytes ; ( 2 ) the matrix of cg might migrate to the surface of plasma membrane of oocytes after pmr pre - treatment. ( 3 ) incubation of zona - free hamster eggs with spermatozoa pretreated with pmr a ntiserum was associated with a dose - dependent significant reduction in the number of spermatozoa binding to the oolemma, compared with a contraband with a dose - dependent reduction of the fertilization rate and the penetration index. the results indicate that mr of human sperm plays an important role in sperm - oocyte fusion and oocyte activation

    結果顯示： （ 1 ）人精子mr能夠誘發去透明帶金黃地鼠卵母細胞中皮質顆粒的胞吐，並引起卵母細胞膜表面微絨毛形態和數量發生改變； （ 2 ）皮質顆粒胞吐后，其內容物可結合到卵膜表面； （ 3 ）抗mr抗血清預處理精子可抑制精子與卵子的結合與融合，使受精率、穿透指數和結合指數下降，並均有劑量依賴性。以上結果提示，人精子mr在受精過程中起重要作用，其主要作用可能是介導精-卵膜融合併促進卵母細胞的活化。
  14. ( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1. 2 gene promoter. these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants. the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene, and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling

    （ 2 ）對該啟動子的5 』端進行不同長度的缺失突變，突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明，該啟動子中- 300 - 243bp區域（有一個gccbox和一個gbox - likesequence ）是其應答meja處理所必須的區域，並將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。
  15. ( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter

    （ 3 ）在缺失突變的基礎上，通過對gccbox及其相鄰的上下游六個堿基進行取代突變，將突變啟動子與gus構建融合基因，在煙草中受heja誘導的瞬時表達結果表明， h1和m3的突變對該啟動子應答ja信號的影響很小，而m2 （ gccbox的突變）則幾乎使該啟動子應答ja信號的功能完全喪失，所以gccbox是該啟動子中應答ja信號的必需元件。
  16. Bsp ( boyine seminal plasma ) protein is the cardinal protein in bovine sperm, which can combine with choline or phosphycholine and then adhere to the surface of sperm to promote the sperm capacitation induced by heparin and high density lipoproteins ( hdl ). moreover, bsp protein can combine with heterogeneous collagen, fibrinogen, heparin, calmoduline, igf - ii and apoa - i. bsp can also inhibit the migration of cholesterol on the sperm membrane

    前言bsp （ bovineseminalplasma ）蛋白是牛精漿中的主要蛋白質， bsp蛋白可以與膽堿或磷脂膽堿結合，黏附於精子表面，可促進由肝素、 hdl等誘導的獲能過程，另外bsp蛋白與不同來源的膠原、纖維蛋白原、肝素、鈣調蛋白、 igf - 、 apoa -等結合， bsp蛋白還可抑制精子胞膜上的膽固醇遷移，這些都暗示了bsp蛋白在精子胞膜的脂類代謝及調節精子的獲能以及頂體反應方面發揮重要作用。
  17. By combining the information from the cdna sequences with functions of their homologs, we suggest that two kinds of hormones may play important roles during flower regeneration in a few ways : ( i ) proteolytic system of ubiqutin / 26s proteosome complex, ( ii ) actin - dependent vesicular cycling of auxin efflux carrier, ( iii ) regulating transcription of genes, ( iv ) phosphorylation and dephosphorylation

    經過進一步的篩選，結合序列特徵和同源基因的功能信息對這些基因功能進行了初步分析。認為兩種外源激素可能通過泛素26s蛋白酶體復合體對調節蛋白的降解機制，生長素運輸載體肌動蛋白依賴的小泡運輸，基因轉錄調節，磷酸化和去磷酸化等調節過程誘導風信子花分生組織的再生。
  18. Results steroid receptor coactivators are essential for several kinds of steroid hormones binding to steroid receptors, so they are important accessory factors that induce the initiation, development and recurrence of breast cancer, and predictive factors that estimate the prognosis of breast cancer

    結果類固醇受體輔活化因子是多種類固醇激素與類固醇受體結合併發揮生物學活性的必需因子，是誘導乳腺癌發生、發展和復發的重要輔助因子，同時也是判斷乳腺癌預后的重要預示因子。
  19. This part emphasizes the synthesis of nanoarrays, aiming at controlling the size and distance of nanocrystallites using calixarene derivatives by altering the size, length and chemical structure of the organic molecules ; 2. this part emphasizes in situ synthesis strategy for fabrication of polymer network of zns based nanopowder, aiming at size controls, coating and preventing agglomeration following " one - pot " synthesis ; this method fits to low cost, large scale production ; 3. according to development in zno nanomaterials, we first report on the synthesis, characterization of amorphous zno, aiming at describing the principles and approaches of synthesis techniques, optical properties, spatial structure and doped effect ; the amorphous zno displays cage - like structure, showing a strong ultraviolet emission while the visible emission is nearly fully quenched, a potential uv - emission material ; 4

    本論文以量子結構自組裝為出發點，提出利用杯芳烴及其衍生物的化學受限反應實現尺寸可調半導體納米粒子自組裝；提出有機聚合網路原位組裝zns基納米熒光粉方法，把熒光粉的納米化、包敷、防團聚在「一鍋」反應中完成，適于低成本，批量生產；根據當前zno的研究情況，我們首次合成了非晶zno ，研究了它的光學性質，確定了它的結構，並對其摻雜進行了初步的研究，非晶zno表現出強的深紫外發光特性，而可見發射非常弱，是一種有巨大潛在應用價值的深紫外發光材料；利用非晶zno的亞穩特性，對晶化過程中非晶zno納米晶zno三維受限量子結構特性，界面特性進行了深入的研究；利用固相熱分解一般受擴散控制特性，實現了尺寸可控的zno三維量子結構的自組裝；利用非晶zno的高度分散性，容易均勻成膜特性，實現了非晶籽晶誘導低溫液相外延自組裝生長高取向zno晶體薄膜。
  20. Induced either by iptg or l - tryptophan in the absence of easily metabolized carbon such as glucose the strain bl21 ( de3 ) / pet28c - tnaa can express tryptopanase. the fermentation conditions were optimized respectively. in the presence of glucose and iptg as induce agent, the concentration is the crucial factor for expression of active tryptophanase. if the iptg concentration is less than 0. 2mm, the optimum temperature is 37 lower temperature is necessary to obtain active tryptophanase in the case of higher concentration of iptg

    用iptg誘導表達實驗結果表明：利用t7啟動子在胞內能表達出有活性的酶； iptg與溫度的合適組合可以得到較高活性的色氨酸酶，在0 . 2mm以下濃度的iptg誘導時， 37是最適溫度，而用較高濃度的iptg誘導，低溫是表達有活性的色氨酸酶的必要條件，而且誘導時間要短。
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