結核桿菌培養 的英文怎麼說
中文拼音 [jiēhégǎnjūnpéiyǎng]
結核桿菌培養
英文
tubercle bacillus culture- 結 : 結動詞(長出果實或種子) bear (fruit); form (seed)
- 核 : 核構詞成分。
- 桿 : 桿名詞(桿子) pole; staff
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 結核 : 1. [醫學] tuberculosis; consumption; phthisis; noddle 2. [地質學] concretion3. [礦物學] nodule
- 桿菌 : [微生物學] bacillus
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In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定,該基因為雞il - 2基因,其序列與sundick等報道的完全一致。在此基礎上,我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ ( tm ) ht中,構建重組表達質粒並進行確證性序列測定,重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ ( tm ) ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養, sds - page和westernblot分析顯示,表達的雞il - 2融合蛋白分子量約為18kda ,表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象,選用真核表達質粒pci - neo ,根據已構建好的含54位密碼子突變型mbl基因t載體的結構,設計合成新的引物, pcr擴增54位密碼突變型mbl基因,凝膠回收,雙酶切pcr產物和pci - neo質粒, t4連接酶連接,將前者克隆至後者的sma和sal位點,轉化大腸桿菌xl - 1blue ,氨芐選擇培養。
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