維系胞胎 的英文怎麼說
中文拼音 [wéixìbāotāi]
維系胞胎
英文
support the fetus-
Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining
（ 3 ）人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞（ primordialgermcells ， pgcs ） ，培養在添加人重組白血病抑制因子（ lif ） 、人重組堿性成纖維細胞生長因子（ bfgf ）和forskolin的小鼠飼養層細胞上。We get to the conclusions : ( 1 ) the histon acetylation level has some derease in the lung of cloned cattle ; ( 2 ) in the cloned cattle xiaobai, the histone acetylation level is lower than that in normal control, however, higher or adjacent to that in other dead cloned cattle ; ( 3 ) there is no significant difference during those cattle from the cumulus, fetal fibroblast and adult fibroblast cell donor
我們發現： （ 1 ）在克隆牛中組蛋白乙酰化水平基本上都有所降低； （ 2 ）克隆牛xiaobai乙酰化水平比正常牛低，而基本上是高於或接近死亡克隆牛水平的； （ 3 ）在我們所研究的三個供體細胞系（卵丘細胞，成體成纖維細胞和胎兒成纖維細胞）中，乙酰化水平無明顯差異。Gonadal development and fecundity are affected by certain essential dietary nutrients, especially essential amino acids, essential fatty acids, and vitamins etc. thus, during the last two decades, more attention has been paid to the optimal level of different nutrients in broodstock diets. it is well known that protein is a kind of indispensable nutrient because of its important physiological function on body structure and supplying energy to the growth and development of shrimp and crab. protein and amino acids of broodstock diet have been identified as major dietary factors that determine successful reproduction and survival of offspring
而這些問題的解決則要求對中華絨螯蟹生殖過程中的攝食和消化生理機制，親體性成熟期對各種必需營養素（如必需氨基酸、必需脂肪酸和維生素等）的需求，卵細胞發生過程中營養物質的合成、轉運及貯存規律，以及在受精卵、胚胎和后續以內源性營養為主的?狀幼體發育階段對卵黃磷蛋白的消耗和利用模式等環節進行較為深入的研究，在全面系統地了解中華絨螯蟹的生殖營養生理的基礎上，探討親體生殖營養對卵質、孵化及后續幼體生長發育的影響。Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation, development of the organisms, cell transformation and oxidative stress. till now, the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated, nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported. this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed, thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively. in this way, we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly, which will contribute a lot to further analysis
鑒于plc - 1發揮上述作用的機制尚未完全闡明， plc - 1通路與其他信號通路間的交聯和代償尚無系統報道，又因為以往的研究方法不夠全面，本研究以野生型小鼠胚胎成纖維細胞（ plc - 1 ~ （ + / + ） ）和缺失磷脂酶c - 1的小鼠胚胎成纖維細胞（ plc - 1 ~ （ - / - ） ）為研究模型，在眾多蛋白組學策略中選擇了雙向電泳+質譜（ 2 - de + ms ）作為研究手段，通過對比表皮生長因子（ egf ）刺激24小時後上述兩種細胞的總蛋白質表達差異，全面地探討敲除plc - 1對生長因子誘導的信號傳遞的影響，從而間接研究plc - 1生物學作用、信號傳遞機制及其代償情況，為后續的深入研究打下基礎。L. a total of 312 fetuses were collected from 27 wuzhishan inbreeding pigs ( wzsp ) and white minitype pigs to study the factors influencing the establishment of porcine embryonic germ cells, and we draw the following conclusions : ( 1 ) we can get embryonic fibroblasts from d26 - 28 fetuses ( day of first mating is day 0, that is d 0 ) ; in this thesis, sto and mef1 were inactivated by incubation in medium containing 10 g / ml of mitomycin c for 1. 75h ~ 2h ; while pef were inactivated by the same medium for 2. 25h ~ 2. 5h
本實驗以中國近交系五指山小型豬（ wzsp ）和微型白豬為材料，先後從27頭豬採集了312枚早期胎兒用於進行豬胚胎幹細胞（ eg ）建系影響因素研究，得出如下結論： （ 1 ） d26 - 28豬胎兒可用於製作胎兒成纖維細胞。製作飼養層時， sto 、 mef ~ 1細胞用10 g ml絲裂酶素c處理1 . 75h 2h ； pef細胞用絲裂酶素c處理2 . 25h 2 . 5h 。We also investigated the factors including feeder layer cells, growth factors and complements, which may affect the cultivation and differentiation of these cells in vitro. the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers
主要內容包括： （ 1 ）小鼠胚胎成纖維細胞（ mef ）的分離培養和飼養層細胞的製作以妊娠13 . 5天或14 16天的小鼠為材料，建立了mef分離培養體系。分享友人