維達隆 的英文怎麼說
中文拼音 [wéidálōng]
維達隆
英文
vidalon-
Dacron polyester fibers
達克隆聚脂纖維Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned
乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。Alonso ' s seville run will take place on a specially - constructed course 2. 5 kilometre course, running from the glorieta de san diego to the glorieta de mejico, and back again
阿隆索在塞維爾的表演將從一條特別修建的2 . 5公里跑道開始，從聖地亞哥廣場出發，最後到達梅吉科廣場，然後再返回。Then daguenet added a rapid word or two about the rest. there was clarisse besnus, whom a lady had brought up from saint - aubin - sur - mer in the capacity of maid while the lady s husband had started her in quite another line. there was simonne cabiroche, the daughter of a furniture dealer in the faubourg saint - antoine, who had been educated in a large boarding school with a view to becoming a governess. finally there were maria blond and louise violaine and lea de horn, who had all shot up to woman s estate on the pavements of paris, not to mention tatan nene, who had herded cows in champagne till she was twenty
隨后，其餘女人的情況達蓋內就三言兩語地說一下：克拉利瑟貝尼，是被一個太太從海濱聖歐班帶來作女僕的，后來那個太太的丈夫把她送出來當了煙花女西蒙娜卡比羅什是聖安托萬郊區的一個傢具商的女兒，在一所很大的培養小學教員的寄宿學校里長大瑪麗亞布隆路易絲維奧萊納和萊婭德霍恩都是被迫走上巴黎街頭，淪為娼妓的。5 - day only arrive at catalina in the morning. this lovely island just off the coast of los angeles is famous for its beauty and tranquility
上午八時抵達卡特琳娜島catalina island ，游覽此島之心臟地帶阿維隆avalon 。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。Leaders and honored guests attending the opening ceremony include princess maha chakri sirindhorn from thailand, zhang xinsheng, vice - minister of the education department of china, chen haosu, president of the chinese peoples association for friendship with foreign countries, professor khunying suchada kiranandana, president of chulalongkorn university of thailand, professor min weifang, director of administrative committee of peking university, and professor hao ping, vice - president of peking university, etc
應邀出席開幕式的領導和貴賓有，泰國詩琳通公主教育部副部長章新勝中國對外友好協會會長陳昊蘇泰國朱拉隆功大學校長坤仁素茶達吉臘南教授北京大學校務委員會主任閔維方教授北大副校長郝平教授等。Restin was a novel gene isolated by our lab from differentiation tumor cell induced by all - trans retinoic acid in 1999, and its molecular functions were not well understood. it mainly expresses in terminally differentiated cells. by transfection of restin into tumor cell, the cells were arrested in g1 phase and the cell proliferation was inhibited
Restin是1999年本室在研究全反式維甲酸誘導細胞分化時克隆得到的一個人類未知功能的新基因，該基因主要表達于終末分化細胞，穩定轉染該基因的細胞出現g1期阻滯，抑制細胞的增殖，提示restin與細胞周期的調控有關。If calderon is elected then he has vowed to bring former player pedja mijatovic back to the club as sporting director and he too admits an admiration for carvalho
卡爾德隆宣稱如果他當選了主席，則要將前皇馬球星佩德加?米亞托維奇帶來，並成為俱樂部新的體育總監。後者也表達了對卡瓦略的興趣。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。The lactase gene from kluyveromyces lactis was researched in the thesis. the cloned gene was expressed in e. coli and the properties of lactase was determinated. in addition, we studied the expression of lactase gene in the methylotrophic yeast pichia pastoris
本論文從一株乳酸克魯維斯酵母菌中克隆獲得乳糖酶基因，在大腸桿菌中進行表達並測定其酶學性質，同時也對利用巴斯德畢赤酵母（ pichiapastoris ）系統表達該基因進行了探索。In order to patriate constitution from the british parliament, several proposals had been made. but all of them failed at last because of the opposition from quebec. queb6cois parti that first came to power in quebec held a referendum over " sovereignty - association " plan in 1980
為收回憲法，聯邦與各省先後達成《富爾頓方案》 、 《富爾頓?法弗隆方案》以及《維多利亞憲章》修憲協議，但由於魁北克省的反對而最後功虧一簣。分享友人