纖維分解酶 的英文怎麼說
中文拼音 [xiānwéifēnjiě]
纖維分解酶
英文
cellulolytic enzyme- 纖 : 纖形容詞(細小) fine; minute
- 維 : Ⅰ動詞1 (連接) tie up; hold together; link 2 (保持; 保全) maintain; safeguard; preserve; keep ...
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 解 : 解動詞(解送) send under guard
- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 纖維 : fibre; staple; filamentary
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Study on the screening of cellulase strains for distiller biomass degradation
分解酒糟生物質的纖維素酶生產菌的篩選研究The peak times of amylase, cmc enzyme, cellulose, laccase, guaiacol oxidase and polyphenol oxidase were the 10th day, the 12th day, the 12th day, the 14th day, the 16th day and the 16th day respectively. it indicated that phellinus igniarius has the capability of discomposing amylum, cellulose and lignose
澱粉酶、 cmc酶、纖維素酶、漆酶、愈創木酚氧化酶、多酚氧化酶的活性高峰分別出現在第10d 、第12d 、第12d 、第14d 、第16d 、第16d ,說明桑黃對澱粉類物質、纖維素類、木質素類物質均具有降解能力。These enzymes are incapable of breaking down or digesting wool, hair or silk fibres which are to them indigestible protein
這些酶無法分解羊毛,動物毛發或絲等其它對於它們來說無法消化的纖維。This paper is a general review of the determination of enzyme activity in cellulose - decomposing bacteria & the development and utilization of wheat bran
本文就纖維分解菌的酶活測定及麩皮的開發和利用進行綜述Purification and characterization of a cellobiohydrolase from the thermophilic fungus chaetomium thermophilum ct
嗜熱毛殼菌外切葡聚糖纖維二糖水解酶的純化和部分性質研究The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。Characteristic of respective components from cellulase system induced by aspergillus niger and condition of enzymolysis
黑麴黴產纖維素酶系各組分特性及酶解條件Its km for sacilin at50 andph5. owas3. 73mg / ml. with the analysis of kinetics, the p - glucosidase showed synergistic action to the endoglucanase and the endoglucanase inhibited the p - glucosidase. the mechanism for their interaction was explained that the endoglucanase was combined with its products and the inhibition of these products to endoglucanase was removed by its hydrolysis by p - glucosidase
經兩組分共同作用的酶反應動力學分析后發現,兩組分這種相互作用機制的核心是:內切酶和?葡萄糖苷酶與內切酶的水解產物,也是其底物類似物? ?纖維二糖和纖維寡糖的結合以及-葡萄糖苷酶對該產物的水解。According to the properties of litter substrate, the enzymes are classified into cellulolytic enzymes, ligninolytic enzymes, proteolytic enzymes and phosphatase
根據森林凋落物底物性質的不同,將凋落物分解酶分為纖維素分解酶類、木質素分解酶類、蛋白水解酶類和磷酸酶類。3. 2. 1. 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25, and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography. the other was a p - glucosidase ( ec. 3. 2. 1. 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography. the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433. 38 hj / mg
-葡萄糖苷酶對水楊素的比活力為597 . 12iu mg ,並對其專一,不能水解棉花和羧甲基纖維素鈉;分子量為117 . 5kda ,加dtt後分子量不變;該組分最適ph和溫度分別為4 . 5和70 ,在ph5 . 0 、 50下對水楊素鈉的米氏常數km為3 . 73mg ml ,最大反應速度vm為0 . 088mg葡萄糖( ml ? min ) ;與文獻中從黑麴黴中分離的-葡萄糖苷酶比較后發現,該組分是一個新的-葡萄糖苷酶。The results indicated that the yields of water - soluble dietary fiber from rice bran by direct water extraction, enzymatic hydrolysis and biological fermentation were 10. 78 %, 16. 32 % and 10. 28 % respectively, and the method of enzymatic hydrolysis was more suitable for preparation of water - soluble dietary fiber
結果表明:直接水浸提法、酶解法和生物發酵法水溶性膳食纖維得率分別為10 . 78 % 、 16 . 32 %和10 . 28 % ,酶解法更適合制備水溶性膳食纖維。The fungus age, enzyme system, osmotic stabilizer, ca2 + concentration influenced on the formation of the protoplast have been confirmed. the result show that 16 - 18hr fermention, 32 1. 0 % cellulase plus 1. 0 % snailase confected by pba ( phosphate blend ammonium chloride ) contant 0. 2 % ca2 +, acted 3 hr is optimal for the protoplast production of aspergillus. niger
分別採用單因子法和正交實驗法考查,認為16 18hr菌齡的菌絲體, 32 ,用含0 . 2 ca ~ ( 2 + )的pba高滲緩沖液配製的1纖維素酶與1蝸牛酶的混合酶酶解3hr ,得到的原生質體數最多。Effect of yeast cultures on fibrolytic bacterial population and activities of fiber hydrolytic enzymes in the rumen
添加不同酵母培養物對瘤胃纖維分解菌群和纖維素酶活的影響Recently, people have being researched enzymic hydrolysis for cellulose due to the development of biotechnology and the high efficiency of enzyme
近年來,隨著生物技術的迅速發展以及具有催化高效性的酶的研究,人們開始試圖利用纖維素酶來水解纖維素,以期達到充分利用的目的。To isolate and purify dnaase in the earthworm first, the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer. then dnaase was purified by denaturing the protein with higher temperature. the following steps were ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane
雙胸蚓組織中dna酶的分離純化採用蔗糖溶解雙胸蚓,並選擇性酸變性制備雙胸蚓組織粗提取液,再經選擇性熱變性、硫酸銨分段鹽析、 deae ?纖維素( de52 )柱層析及超濾膜分級分離對雙胸蚓組織中dna酶進行分離純化。Nattokinase ( nk ) is a new fibrinolytic enzyme which cleaves directly cross - linked fibrin in vitro / in vivo
納豆激酶是一種能夠在體內及體外直接分解交聯纖維蛋白的新型溶血栓酶。The physiological character of cellulase and the best culture ph, temperature and stability were quested for. then according to this base, this thesis analyzed the influence of some parameters, including energy and dose for the purpose of finding the best ion mutant parameters, and studied the mechanism of implanted ion on this strain
選擇了纖維分解細菌酶活測定方法以及dns法測定還原糖的最適條件,對纖維素分解細菌的生長特性及纖維素酶的最適溫度、 ph值、溫度和ph值的穩定性進行了探索。1 examined decomposition rate of filter paper, cmc enzyme and crude cellulose enzyme of bacteria
篩選出對天然纖維素分解能力較強的菌株共5株,酶活力在60 . 00mg ml ? d以上。The research advances of molecular structure and the degradation mechanism of cellulases separated horn microorganism and animal west summarized
摘要綜述了近年來微生物纖維素酶和動物纖維素酶分子結構和降解機制方面的研究進展。分享友人