缺失突變體 的英文怎麼說

中文拼音 [quēshībiàn]
缺失突變體 英文
deletion mutant
  • : Ⅰ動詞1 (缺乏; 短少) be short of; lack 2 (殘缺) be missing; be incomplete 3 (該到而未到) be ...
  • : Ⅰ動詞1 (猛沖) dash forward; shoot out 2 (高於周圍) protrude; bulgeⅡ副詞(突然) abruptly; sud...
  • : 體構詞成分。
  • 缺失 : hiatus; deficiency; [遺傳學] deletion
  1. Two magnetosome deletion mutants were constructed by conjugative transposon mutagensis and the application of this genetic system. the two magnetosome deletion mutants were named as nm4 and nm21 respectively

    並利用此系,通過接合轉座誘技術,獲得了2個磁小株: nm4 、 nm21 。
  2. In most wild type e. coli strains containing heterologous pha synthase genes, the amount of pha accumulation was almost undetectable while in e. coli km32b the amount of pha accumulation were clearly visible

    在多種野生型大腸桿菌中,盡管使用了各種不同的表達載,也很難獲得可供檢測的pha ;但應用大腸桿菌的fqdb株卻可以獲得較多量的pha 。
  3. Here we found g proteins also function in leaf, silique development and the yield of pollen microspore. we observed several traits or characters in the offsprings of gpal, agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type, whereas the lamina length, petiole length and rosette diameter is smaller than the wild type, the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type, and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines

    實驗中我們跟蹤觀察了多代異三聚g蛋白a亞基超表達轉基因植株及a , p亞基缺失突變體的表型特徵,發現的葉片寬度大於對應的野生型,葉片長度,葉柄長度及蓮座直徑小於野生型,而超表達植株的上述某些特徵與相反; gp時的長角果長度,花梗柄部長度大於野生型,而超表達ga植株種英則略小於對照; gpal長角果尖端未出現咭乙i的特徵: gpal ,口gbl花粉生成量大於野生型,而超表達ga植株的花粉生成量則略小於對照。
  4. We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression

    對其進行,構建5個表達載轉基因擬南芥,葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達,說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。
  5. Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions

    細胞色素b基因序列異分析表明: 1 )雁形目鳥類細胞色素b基因全序列長度一致,無插入和:對照雞線粒密碼子系統全序列能全部翻譯成氨基酸序列,無無義,全序列內部無終止密碼子; 2 )序列比對后1143加,含416個核著酸異位點, 306個簡約信息位點,其中處於密碼子第三位的異最大,第一位和第二位堿基的異相對較小。
  6. It is possible that exogenous dna fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level

    這可能是外源dna片段整合進受的基因組中,改基因的順序或者引起基因堿基的、插入,從而在基因水平上發生
  7. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶( exonuclease , exo )順序,獲得單向長度漸減重疊的系列,核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps , frameplot程序分析揭示出該區域一個完整的開放閱讀框( orf )的存在,其大小為1656bps , g + c含量為70 . 3 ,編碼552個氨基酸,利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較,發現無論在核苷酸水平還是在蛋白水平上,該orf均與膽固醇氧化酶表現出同源性,而且與鏈黴菌膽固醇氧化酶同源性最高,說明該orf編碼膽固醇氧化酶基因。
  8. The availability of a newly selected aba - deficient arabidopsis mutant ios5 makes it convenient to study aba signaling

    新篩選的擬南芥aba缺失突變體los5為研究aba信號轉導提供了便利的條件。
  9. It indicated that the magnetosome deleted phenotype w as caused by tn5 insertion

    Southern雜交驗證磁小確為tn5插入所致。
  10. Ie protein have multiple functions, such as conversion of the host cell into a metabolically activate states, activation of viral early genes in the early phase, repression of their own transcription. ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein

    經分析偽狂犬病病毒立即早期蛋白( immediate - earlyprotein180 , ie180 )的蛋白質結構,發現ie180具有轉錄激活因子的結構特徵,能與類rna聚合酶結合, ie180基因缺失突變體對sv40及cmv啟動子調控作用的研究,是考察ie180能否作為轉錄激活因子應用的關鍵。
  11. Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe

    對片段進行克隆、亞克隆之後測序分析,發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性;並且三次獨立的pcr均得到相同的片段,所克隆的基因序列與choe相比,連續三個堿基( ttc ) ,即相應的氨基酸序列一個氨基酸( phe ) ,因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然,命名為choew 。
  12. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載與具有高度感染性的bartha - k61株基因組dna通過脂質加plus法共轉染vero細胞,採用甲基纖維素固定病, x - gal染色,經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因株,命名為rprv - lacz 。
  13. The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction. marker exchange method was used to study the relationship between the production of protease and the 3 orfs. a deletion mutant of xcc _ 4463 was constructed successfully

    Orf注釋表明,共中一個基因為胞外蛋白酶結構基因, 3個基因與合成調控有關, 5個與轉運分泌有關, 2個與lps合成有關,其餘3個orf的注釋功能與胞外蛋白酶的關系未見報道,為研究這3個orf與胞外蛋白酶產生的關系,採用同源雙交換orf法進行了進一步驗證,成功地構建了xcc _ 4463缺失突變體,所得缺失突變體經檢測胞外蛋白酶減少,在寄主上致病性降低。
  14. By analyzing the wing phenotype, cuticle phenotype of germline clone embryo, deficiency kit mapping, direct sequencing and searching the genome sequence database, three genes : sll, oxt and pygo were identified ( each gene corresponding to one group of mutant alleles )

    並通過綜合分析翅的形態,生殖系克隆胚胎的表皮形態,以及作圖,直接測序和搜索基因組數據庫,鑒定出了三個基因,這三個基因分別對應于該三類等位基因。
  15. Based on the results that were investigated on the mutants etr1, a member of ethylene receptor genes, etr1, ers1, etr2, ein4, ers2, ethylene receptor genes might play a negative role in regulating response to ethylene

    Etr1是五個乙烯受基因家簇( etr1 , ers1 , etr2 , ein4 , ers2 )中的一員,對他們的功能缺失突變體研究表明他們在乙烯受系統中可能是起負調控作用。
  16. The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1. using erase - a - base system, a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster

    將add基因簇亞克隆到測序載pbluescript sk ( + )上,採用exo法得到了66個單向遞減亞克隆,然後對這些克隆進行測序。
  17. Cat ( chloramphenical acetyl transferase ) elisa assays to identified the function of constructed plasmid. the result indicates mutant p1p2, p1p3p2 and p6p2 repressed to cmv promoter. same as ie promoter two special sequences " atcgt " located at two side of the transcription initial site of promoter

    共轉染hplp細胞,結果表明所有的缺失突變體對cmv啟動子都表現抑制活性,我們發現在ie180和p啟動子的轉錄起始位置兩側同時具有兩個特徵序列( 5 』 atffit 3 』 ) 。
  18. The deletion mutants were proved to be genetically stable, and thus might be promising strains in industrial production of avermectins b. although s. avermitilis produces eight related components of avermectins, only two components, bla and bib, are available for the medical, veterinary and agricultural fields

    是在染色上通過同源雙交換完成的,不會發生進一步的重組,因此株性狀穩定,在工業生產上具有應用價值。阿維菌素的天然發酵產物共有八個組分,其中只有b1組分的殺蟲活性最高,被作為殺蟲劑在農業和畜牧業中使用。
  19. It was devoted that class ii promoter was stimulated by ie180. there is not tata box on the upstream of the promoter, but there are binding sites of protein in the upstream of the promoter. david and his company identified ie gene in the human cytomegalovirus virus dna segment

    目前國內外沒有關於野生型的ie180或其對sv40和cmv啟動子調控作用的報導,此研究可為ie180基因缺失突變體,在人的巨噬細胞病毒和猴空泡病毒40控制方面的應用提供理論依據。
  20. The level of cytosolic free calcium in gp mutants is not different. adding exogenous purified cam to the cultural medium increased the pollen germination, tube growth, and cytosolic free calcium concertration in both wild type and ga mutant. however, the former was more sensitive to exogenous cam than the latter

    外源鈣調素(花椰菜和擬南芥11亞型, 10一7m )處理能夠提高野生型及gpol缺失突變體中的游離鈣離子水平,但對野生型花粉細胞游離鈣離子水平的激活效應高於gpal缺失突變體
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