缺失突變 的英文怎麼說

中文拼音 [quēshītūbiàn]
缺失突變 英文
deletion mutation
  • 缺 : Ⅰ動詞1 （缺乏; 短少） be short of; lack 2 （殘缺） be missing; be incomplete 3 （該到而未到） be ...
  • 突 : Ⅰ動詞1 （猛沖） dash forward; shoot out 2 （高於周圍） protrude; bulgeⅡ副詞（突然） abruptly; sud...
  • 缺失 : hiatus; deficiency; [遺傳學] deletion
  • 突變 : 1 （突然急劇的變化） sudden change; change suddenly; transilience; accident; saltation; revulsion...
  1. Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins

    將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。
  2. Two magnetosome deletion mutants were constructed by conjugative transposon mutagensis and the application of this genetic system. the two magnetosome deletion mutants were named as nm4 and nm21 respectively

    並利用此體系，通過接合轉座誘變技術，獲得了2個磁小體缺失突變株： nm4 、 nm21 。
  3. In most wild type e. coli strains containing heterologous pha synthase genes, the amount of pha accumulation was almost undetectable while in e. coli km32b the amount of pha accumulation were clearly visible

    在多種野生型大腸桿菌中，盡管使用了各種不同的表達載體，也很難獲得可供檢測的pha ；但應用大腸桿菌的fqdb缺失突變株卻可以獲得較多量的pha 。
  4. Here we found g proteins also function in leaf, silique development and the yield of pollen microspore. we observed several traits or characters in the offsprings of gpal, agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type, whereas the lamina length, petiole length and rosette diameter is smaller than the wild type, the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type, and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines

    實驗中我們跟蹤觀察了多代異三聚體g蛋白a亞基超表達轉基因植株及a ， p亞基缺失突變體的表型特徵，發現突變體的葉片寬度大於對應的野生型，葉片長度，葉柄長度及蓮座直徑小於野生型，而超表達植株的上述某些特徵與突變體相反; gp時突變體的長角果長度，花梗柄部長度大於野生型，而超表達ga植株種英則略小於對照; gpal突變體長角果尖端未出現咭乙i突變體的特徵: gpal ，口gbl突變體花粉生成量大於野生型，而超表達ga植株的花粉生成量則略小於對照。
  5. We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression

    對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。
  6. Microcalorimetric study on deletion mutagenesis of the gene promoter sequences from the extremely halophilic archaea

    極端嗜鹽古生菌啟動子序列缺失突變的微量熱研究
  7. 2 ) dna sequence variations were all nucleotide substitutions and neither insertions nor deletions were detected

    2山na序列變異均為核耷酸替換，無插人和缺失突變。
  8. The availability of a newly selected aba - deficient arabidopsis mutant ios5 makes it convenient to study aba signaling

    新篩選的擬南芥aba缺失突變體los5為研究aba信號轉導提供了便利的條件。
  9. It indicated that the magnetosome deleted phenotype w as caused by tn5 insertion

    Southern雜交驗證磁小體缺失突變確為tn5插入所致。
  10. Ie protein have multiple functions, such as conversion of the host cell into a metabolically activate states, activation of viral early genes in the early phase, repression of their own transcription. ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein

    經分析偽狂犬病病毒立即早期蛋白（ immediate - earlyprotein180 ， ie180 ）的蛋白質結構，發現ie180具有轉錄激活因子的結構特徵，能與類rna聚合酶結合， ie180基因缺失突變體對sv40及cmv啟動子調控作用的研究，是考察ie180能否作為轉錄激活因子應用的關鍵。
  11. ( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1. 2 gene promoter. these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants. the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene, and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling

    （ 2 ）對該啟動子的5 』端進行不同長度的缺失突變，突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明，該啟動子中- 300 - 243bp區域（有一個gccbox和一個gbox - likesequence ）是其應答meja處理所必須的區域，並將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。
  12. ( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter

    （ 3 ）在缺失突變的基礎上，通過對gccbox及其相鄰的上下游六個堿基進行取代突變，將突變啟動子與gus構建融合基因，在煙草中受heja誘導的瞬時表達結果表明， h1和m3的突變對該啟動子應答ja信號的影響很小，而m2 （ gccbox的突變）則幾乎使該啟動子應答ja信號的功能完全喪失，所以gccbox是該啟動子中應答ja信號的必需元件。
  13. In this paper, an important cis - acting element within the promoter of pdf7. 2, which is activated by jas, was analyzed, and the interaction of this element with relevant factors ( jerfs ) was also studied. the results were as fellows : la ja responsive cis - acting element within the promoter of pdf 1. 2 was investigated via comprehensive mutant analysis. ( 1 ) a strategy based on cdna sequence was used to amplify the promoter ofpdf1. 2 gene with arabidopsis gdna as template

    本文對受jas誘導的pdf1 . 2啟動子的順式作用元件及其與jerfs的相互作用進行了系統研究，取得如下結果： 1 、通過對pdf1 . 2啟動子的缺失突變分析和取代突變分析，對該啟動子應答jas信號的順式作用元件進行了較詳細的探討。
  14. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
  15. The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction. marker exchange method was used to study the relationship between the production of protease and the 3 orfs. a deletion mutant of xcc _ 4463 was constructed successfully

    Orf注釋表明，共中一個基因為胞外蛋白酶結構基因， 3個基因與合成調控有關， 5個與轉運分泌有關， 2個與lps合成有關，其餘3個orf的注釋功能與胞外蛋白酶的關系未見報道，為研究這3個orf與胞外蛋白酶產生的關系，採用同源雙交換orf缺失法進行了進一步驗證，成功地構建了xcc _ 4463缺失突變體，所得缺失突變體經檢測胞外蛋白酶減少，在寄主上致病性降低。
  16. Based on the results that were investigated on the mutants etr1, a member of ethylene receptor genes, etr1, ers1, etr2, ein4, ers2, ethylene receptor genes might play a negative role in regulating response to ethylene

    Etr1是五個乙烯受體基因家簇（ etr1 ， ers1 ， etr2 ， ein4 ， ers2 ）中的一員，對他們的功能缺失突變體研究表明他們在乙烯受體系統中可能是起負調控作用。
  17. Two pta - mutants have been selected by using suicide substrate after the mini - tn5 transposon insertion mutagenesis of klebsiella pneumoniae m5al. when used in microaerobic fermentation, the amount of acetate produced by the mutants reduced to less than 50 % of the parent strain, and the yields improved whereas the 1, 3 - propanediol titers and productivities decreased

    以klebsiellapneumoniaem5al為出發菌株，用mini - tn5隨機轉座誘變結合自殺性底物篩選的方法得到了兩株產乙酸途徑pta基因缺失突變株xl - 6和xl - 11 ，應用到微氧法發酵中，突變株的乙酸產量為親株的50以下，甘油轉化率有所提高，但1 ， 3 -丙二醇濃度和生產強度有所下降。
  18. The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1. using erase - a - base system, a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster

    將add基因簇亞克隆到測序載體pbluescript sk （ + ）上，採用exo缺失突變法得到了66個單向遞減缺失亞克隆，然後對這些克隆進行測序。
  19. Cat ( chloramphenical acetyl transferase ) elisa assays to identified the function of constructed plasmid. the result indicates mutant p1p2, p1p3p2 and p6p2 repressed to cmv promoter. same as ie promoter two special sequences " atcgt " located at two side of the transcription initial site of promoter

    共轉染hplp細胞，結果表明所有的缺失突變體對cmv啟動子都表現抑制活性，我們發現在ie180和p啟動子的轉錄起始位置兩側同時具有兩個特徵序列（ 5 』 atffit 3 』 ） 。
  20. The deletion mutants were proved to be genetically stable, and thus might be promising strains in industrial production of avermectins b. although s. avermitilis produces eight related components of avermectins, only two components, bla and bib, are available for the medical, veterinary and agricultural fields

    該缺失突變是在染色體上通過同源雙交換完成的，不會發生進一步的重組，因此突變株性狀穩定，在工業生產上具有應用價值。阿維菌素的天然發酵產物共有八個組分，其中只有b1組分的殺蟲活性最高，被作為殺蟲劑在農業和畜牧業中使用。
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