缺胞核的 的英文怎麼說
中文拼音 [quēbāohéde]
缺胞核的
英文
anucleate-
Cytoarchitectonics of neuronal nitric oxide synthase immunopositve neurons in caudate putame of rats with cerebral ischemia
腦缺血大鼠尾殼核神經元型一氧化氮合酶免疫陽性神經元的細胞構築The centriole lies outside the nucleus of animal cells and many fungal and protoctist cells, but is absent in cells of most higher plants
中心粒存在於動物細胞和許多真菌及原生生物的細胞核外,但在大多數高等植物中缺失。We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression
對其進行缺失突變,構建5個缺失表達載體轉基因擬南芥,葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達,說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system
人骨形成蛋白3 ( hbmp - 3 )屬于tgf -超家族的一員,可以誘導間充質細胞分化為軟骨和骨,在胚胎時期骨骼發育和骨再生修復中起著重要的作用,而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用,因而hbmp - 3有廣闊的市場前景。它在人體內含量極微,盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達,但是由於原核表達系統缺乏翻譯后的加工修飾,真核表達系統存在成本高、產量低等特點,限制了其在臨床上的應用。Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions
細胞色素b基因序列變異分析表明: 1 )雁形目鳥類細胞色素b基因全序列長度一致,無插入和缺失:對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列,無無義突變,全序列內部無終止密碼子; 2 )序列比對后1143加,含416個核著酸變異位點, 306個簡約信息位點,其中處於密碼子第三位的變異最大,第一位和第二位堿基的變異相對較小。Chlorella is one kind of sphere single cell fresh water green alga, it includes the rich protein, the vitamin, the mineral substance, food textile fiber, the nucleic acid and the chlorophyll and so on, it is the maintenance promotes our health essential nutrient
小球藻是一種球形單細胞淡水綠藻,它含有豐富的蛋白質、維生素、礦物質、食物纖維、核酸及葉綠素等,是維持促進我們健康所不可缺少的營養素。In our culture condition, the lepcs express oval cell markers ck 19, ck 14, ov6 and oc. 10, but not oc. 2 and oc. 5. the cells also express c - met, the receptor for hepa tocyte growth factor ( hgf ). antigens traditionally associated with haematopoietic stem cells, including c - kit, thy - 1 and cd 34, can be expressed by oval cells
該細胞系是典型的上皮樣細胞,體外生長時成「鋪路石」樣排布;在電鏡下觀察,細胞核質比大,胞漿中除一些線粒體和核糖體外缺乏其他細胞器;在體外培養時細胞可以保持不分化狀態,表達卵圓膽管細胞的分子標志,如ck14 , ck19 , ov6 , ocChromosome karyotypintg. the classical method in the technology of chromosome genetic analysis, is one of the important means in genetic research and supplementary clinical diagnosis. and it is then key index to analyze chromosome translocation or deficiency, and diagnosis of a variety of genetic diseases. the goal of chromosome analysis is to relate deviations from normal structure to biological or clinical effects
染色體核型分析,染色體遺傳分析技術的經典方法,是遺傳學科學研究和輔助臨床診斷的重要手段之一,是分析染色體易位,缺失,診斷各種遺傳病變的關鍵指標,染色體分析的目的就是要確定細胞或個體的染色體組成,尤其是要將其與正常結構間的偏差和生理的或臨床疾病關聯起來。Effects of antisense oligonucleotide on expression of tissue factor in culturec human umbilical vein endothelial cells injured by anoxia - reoxygention
反義寡脫氧核苷酸對內皮細胞缺氧再復氧損傷組織因子表達的影響Although the thymosin has been successfully expressed in the prokaryotic cell system, its application is restricted because of its deficiency of modification after translation
盡管研究者已在原核細胞表達系統對胸腺肽的表達進行了研究,但由於原核表達系統缺乏翻譯后的加工修飾等缺點,限制了其應用。Clusters of atrophic prostatic acini with proliferatie changes. at low magnification, it may be mistaken for adenocarcinoma ; howeer, they lack cytologic features of cancer such as prominent nucleoli
簇狀萎縮的前列腺腺泡伴發增生性改變。低倍鏡下容易誤當作腺癌,盡管如此,仍缺乏典型的癌細胞特徵,如核仁顯著等。Research of mcp - 1 expression in rat ' s retina injured by ischemia - reperfusion
目的了解視網膜缺血再灌注損傷中不同時期單核細胞趨化蛋白To make clear the hypothesis, a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study, respectively. we first studied the cellular localization of kvl. 2 and the co - localization of kvl. 2 protein and vegf receptors flk - 1 and flt - 1, observed the effect of mcao on kvl. 2 expression and phosphrylation in the rat brain in vivo, then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl. 2 in sh - sy5y cells. finally, in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl. 2 phosphorylation, we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl. 2 phosphorylation
為了驗證上述假設,本文分別在整體和離體水平,採用大腦中動脈缺血( middlecerebralarteryocclusion , mcao )和體外氧?糖剝奪( hypoxiaandglucose - deprivation , hgd )缺血模型,首先了解了kv1 . 2蛋白的細胞定位及與vegf受體flk - 1和flt - 1的共存情況,觀察了整體mcao后缺血再灌不同時間大鼠腦內kv1 . 2蛋白的磷酸化水平變化,然後通過外源性給予vegf蛋白,在sh - sy5y細胞株上觀察其對缺血細胞存活率及kv1 . 2蛋白磷酸化水平的影響,最後利用vegf反義脫氧寡核苷酸( oligodeoxynucleotide , odn )特異阻斷內源性vegf蛋白的表達,觀察內源性vegf蛋白在缺血細胞損傷及調節kv1 . 2蛋白磷酸化中的作用,以進一步明確vegf缺血保護效應與其調節kv1 . 2蛋白磷酸化之間的關系。In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg
同時將原核表達載體pet - 28c用nco , xho雙酶切,回收酶切產物,將回收的酶切產物pea , h3 ,載體進行連接,並轉入dh5感受態細胞內,培養12 - 18小時后,挑取陽性菌落,經nco , xho雙酶切分析及pcr檢測,篩選到陽性克隆,其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。分享友人