肽基合成酶 的英文怎麼說
中文拼音 [tàijīgěchéng]
肽基合成酶
英文
peptidyl synthetase-
Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin
這些基因中有和肝再生有直接關系的如:胎球蛋白、組織蛋白酶;和肝臟功能密切相關的如:胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶;與物質能量代謝有關的如: atp合成酶、核糖體蛋白;以及與急相反應有關的如:觸珠蛋白、轉鐵蛋白。In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method
第一部分,我們按照交替氧化酶( alternativeoxidase , aox )位於線粒體內膜外側親水區s螺旋的氨基酸序列,用固相法合成由12個氨基酸殘基組成的多肽,將此多肽與-牛胰凝乳蛋白酶原a相連,用作半抗原,免疫家兔制備人工合成12肽的抗體。The amino acid sequences of epsp synthase between high plants have very high identity, but the identity of transport peptide of epsp is as low as 23 % between plants
高等植物成熟的epsp合成酶的氨基酸序列之間具有很高的同源性,但epsp合成酶的葉綠體運輸肽的氨基酸序列同源性極差,各植物間僅23左右的同源性。Strain 042bm - x2 was found to contain a single tn5 insertion in the gene smc02682, which showing a high degree of similarity with the rhizobium tropici gshb gene, encoding the enzyme glutathione synthetase. in our experiment, this gene was demonstrated to be related to salt tolerance. so it is named as rst - 0x2
Rst - 0x1基因的功能未知,而rst - 0x2基因和熱帶根瘤菌谷胱甘肽合成酶基因gshb有很高的同源性。 pcr擴增得到包含啟動子的042bmrst - 0x1和rst - 0x2基因的全長。Dna fragments containing rst - 0x1 and rst - 0x2 as well as their promoters were abtained by pcr. the results of sequencing and blast analysis suggested these fragments shares 99 % identity with sinorhizobium meliloti 1021. function of rst - oxl was unknown, while rst - 0x2 shares 81 % identity with gshb gene of rhizobium tropici, which encoding glutathione synthetase
序列測定和blast分析表明,它們和苜蓿中華根瘤菌1021的相應基因有99同源性, 042bm的rst - 0x1基因功能未知,而rst - 0x2基因和熱帶根瘤菌谷胱甘肽合成酶基因gshb有81同源性。Shen hong - yan, tian gui - ling *, zhu wen - jiang, ha sha, ye yun - hua ; application of depbt on the synthesis of protectide dipeptides containing histidine with unprotected imidazole group by solution method, chinese journal of chemistry, 2003, 21, 801 ? 804
沈鴻雁,田桂玲* ,閻愛新,賈艷,洪毅穎,葉蘊華;有機溶劑性質及其水含量對酶催化合成含d -氨基酸殘基的二肽衍生物的影響,化學學報, 2003 , 61 ( 7 ) , 1144 ? 1148An enzymatic synthesis and composition of oligomers and co - oligomers comprised of. alpha. - hydroxy carboxylic acids and. alpha. - amino acids or peptides is disclosed
一種低聚體和由-羥基羧酸和-氨基酸或肽組成的副低聚體的酶合成成分。Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本實驗通過3 』端互補,進行引物延伸合成epo信號肽序列:信號肽和hsblys基因採用重疊延伸拼接法形成融合基因;融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體:引物延伸合成信號肽時,利用亮氨酸同義密碼,將信號肽基因的倒數第二個密碼突變,在重組載體上的信號肽序列之後,形成bln酶切位點,使三種載體成為分泌表達載體。The gene of amoa in ammonia - oxidizing encodes the active - site polypeptide of ammonia monooxygenase which catalyzes the oxidation of ammonia to hydroxylamine. we designed a pair of primers special for the amoa gene by comparing the known amoa gene sequences and used pcr to amplify the amoa gene fragments
Amoa基因是編碼氨單加氧酶活性多肽位點基因,我們通過引物篩選合成了對氨氧化細菌amoa基因特異結合的引物序列,利用pcr技術對活性污泥中的amoa基因片段進行特異擴增,得到的dna片段大約為490bp 。In this study, it has been put forward that taking reactive nanometer magnetic fe304 particles as magnetic nucleus, and the copolymer of styrene ( st ) ? crylic acid ( aa ) as macromolecular shell, we could synthesize, magnetic polymer composite microspheres containing carboxyl groups on their surface, then microspheres are activated by thionylchloride, the surface of such magnetic composite microspheres thus produced had reactive acid chloride groups which then react with the free amino groups of the free soluble enzymes to give peptide bonds ( ? o ? h ?,
本研究首次提出了以納米級磁性fe _ 3o _ 4粒子為核心,苯乙烯( st ) ?丙烯酸( aa )共聚物為高分子殼層,合成了表面帶羧基的磁性高分子復合微球,然後將這種微球用二氯亞碸進行活化處理,在其表面形成了反應性酰氯基團,該基團可以與游離酶的氨基形成肽鍵,從而將游離酶固定化。Objective : polypeptide : n - acetylgalactosaminyl transferase is the initiation enzyme catalyzing the linkage of o - glucan chain. recent study shows that o - glucosylation is closely related to molecular recognition, tumor formation, development and metastasis, as well as embryonic development. due to the initial study on function of o - glucosylation in china, this thesis aims to obtain stably expressed pp - galnac - t2 gene clones for further study
目的多肽: n乙酰氨基半乳糖轉移酶是合成o糖鏈的起始酶,而目前的研究認為, o -糖基化與分子及細胞識別、腫瘤的發生發展和轉移以及胚胎發育等功能密切相關。Construction and expression of tandem multi - copy gene edcoding ca ( 1 - 8 ) me ( 1 - 10 ) hybrid fused acidic peptide to improve antimicrobial peptide ' s expression, a novel mass - production method is proposed. it is based on the neutralization of the positive charges of antimicrobial peptide by fusing to an acidic peptide to avoid the lethal effect of the expressed peptide on the host cells
為提高抗菌肽的表達,設計在抗菌肽基因的n端融合一段編碼酸性短肽的片段作為對抗菌肽前體的模擬,以減輕表達產物對宿主的毒性;同時通過含有酶切位點的接頭將該融合肽基因以同向串連的方式連接成多拷貝基因。While there are some difference in amino acid with other group ( except tw 95 ), the amino acid in 65 - 75 of hn of ndv group are conserved, while he in 81 is conserved. based on epitope prediction, five pairs primers are designed with enzyme sites. five segments of f1, f2, f3, f4 and hn1 coding epitope was amplified and cloned to prokaryotic expression vector, respectively
在表位預測的基礎上,將臨近的表位合併,設計引物並引入酶切位點,以fa 、 fb 、 hna為模板擴增含抗原表位的多肽片段f1 、 f2 、 f3 、 f4 、 hn1 ,克隆到原核表達載體ppro ~ ( ex ) ht上,成功地構建了五個表達載體。In yeast, the synthesis of trehalose is catalyzed by trehalose - 6 - phosphate synthase / phosphatase complex, which is encoded by four genes : tpsl, tps2, tps3 and tsl1
酵母細胞中的海藻糖由tps1 、 tps2 , tps3和tsl1四個基因負責合成,其編碼的多肽組成一個海藻糖合酶復合體。分享友人