Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本實驗通過3 』端互補,進行引物延伸合成epo信號
肽序列:信號
肽和hsblys
基因採用重疊延伸拼接法形成融合
基因;融合
基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體:引物延伸合成信號
肽時,利用亮氨酸同義密碼,將信號
肽基因的倒數第二個密碼突變,在重組載體上的信號
肽序列之後,形成bln酶切位點,使三種載體成為分泌表達載體。
Methods employed to prepare vaccine include rumor cells genetically modified with cytokines, costimulatory molecules and tumor antigenic peptides, dendritic cells ( dc ) primed with tumor antigens in vitro or genetically modified with tumor antigens, or fusion of tumor cells with antigen presenting cells ( apc )
現有的比較受到關注的制備腫瘤疫苗的方法有各種細胞因子、共刺激分子、腫瘤抗原
肽等
基因修飾的腫瘤細胞疫苗;體外抗原致敏的或腫瘤抗原
肽基因修飾的樹突狀細胞疫苗;腫瘤細胞與抗原遞呈細胞融合疫苗等。
3. southern blot analysis of pcr positive plants indicated that 3 plants were transgenic plants and the thymosin gene has been transformed into genome of lettuce. 4
Ctab法提取pcr陽性株的
基因組dna進行southern雜交,其中3株為陽性,表明胸腺
肽基因已經整合到生菜
基因組中。
They inhibits the growth of fungi ( filamentous fungi especially ) while are non - toxic to plant cells. the main results were as follows : 1. obtaining of spcema ( signal peptide modified cema ) spcema ( 187bp ) was amplified with two long complementary primers ( p2 and p3 ) and two primers ( pl and p4 ) containing restriction enzyme recognition site
帶信號
肽cema
基因的pcr合成以兩條部分重疊長鏈引物p _ 2和p _ 3延伸產物為模板, p _ 1和p _ 4為正、反引物進行pcr擴增,獲得了改造的抗菌
肽基因spcema ( 187bp ) 。
2 hz and 100 hz electroacupuncture accelerate the expression of genes encoding three opioid peptides in the rat brain
2hz和100hz電針加速腦內三種阿片
肽基因表達
Construction and expression of tandem multi - copy gene edcoding ca ( 1 - 8 ) me ( 1 - 10 ) hybrid fused acidic peptide to improve antimicrobial peptide ' s expression, a novel mass - production method is proposed. it is based on the neutralization of the positive charges of antimicrobial peptide by fusing to an acidic peptide to avoid the lethal effect of the expressed peptide on the host cells
為提高抗菌
肽的表達,設計在抗菌
肽基因的n端融合一段編碼酸性短
肽的片段作為對抗菌
肽前體的模擬,以減輕表達產物對宿主的毒性;同時通過含有酶切位點的接頭將該融合
肽基因以同向串連的方式連接成多拷貝
基因。
To date, a large variety of antibacterial peptides are expressed in a series of tissues and cells of mammals and some mature peptides with antibacterial activity have been isolated
目前在多種哺乳動物體內的各種組織器官和細胞中都發現有抗菌
肽基因的表達,有的還分離出成熟的活性
肽。
It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin, suggesting that the intracellular compartment may be an important part of the uptake pathway. ricin enters the cells by receptor - mediated endocytosis, followed by translocation across the membranes of intracellular organelles. in this study, a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction
本實驗為重點研究反式高爾
基體在毒素細胞內轉運過程中的作用,我們設計了一個反式高爾
基體的靶向信號
肽基因yqrl ( tyr - gln - arg - leu )連接在rta分子的羧
基端,用原核表達蛋白體系,純化並測定rta - yqrl與rta蛋白對hela (人宮頸癌細胞) , wish (人羊膜上皮細胞) , skov - 3 (人卵巢癌細胞)三種細胞的毒性作用。
Cloning and sequence of mature peptiede of human bone morphogenetic protein7cdna
7成熟
肽基因克隆及序列測定
Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site, and the igf - i product of pcr contains 230 base pairs. igf - ii contains 219 base pairs. 3
各另外設計一對特異性pcr引物,導入適當限制性內切酶切點,以上述連有目的
基因的克隆載體為模板,採用pcr方法擴增
基因片段,獲得長度約230bp的igf -和219bp的igf -成熟
肽基因序列。
Secreted expression of mature peptide gene of human bone morphogenic protein - 7 in pichia pastoris
7成熟
肽基因在巴斯德畢氏酵母中的分泌表達
Cloning of variable region and signal peptide genes of anti - cd20 monoclonal antibody by rlm - race
20單克隆抗體可變區
基因及其信號
肽基因
Construction of plant binary expression vector peasp in consideration of the different cell membrane disruption mechanism of plant defensin and cema, plant binary expression vector peasp were constructed. simultaneous expression of cema and afp may synergistically inhibit the growth of phytopathogens that are the main cause agent of agricultural loss
構建了雙價抗菌
肽基因植物表達載體peasp鑒于植物防禦素與cema的空間結構、作用機制有所不同,兩者在植物中的同時表達,有可能對病源真菌有協同抑制作用,構建了雙價植物表達載體peasp 。
Haemostaseology - determination of protein c activity - reference measurement procedure with a chromogenic peptide substrate
止血學. c蛋白活性的測定.
肽基色譜參照測量程序
The subject in this study is the recombination, expression, purification and biological activity on antibacterial fusion peptide of, magainin - protegrin
本實驗主要研究的是magainin - protegrin雜合抗菌肽基因工程菌的構建、表達、純化及活性測定。