肽測序 的英文怎麼說

中文拼音 [tàicèxù]
肽測序 英文
peptide sequencing
  • 肽 : 名詞[化學] （有機化合物） peptide
  • 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
  1. Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4

    Sds page分析得到3條主要蛋白帶，剪下這三條帶進行膠內賴氨酸內切酶的消化，通過高效液相色譜分離肽段，選擇性進行肽段的氨基酸序列測定。
  2. The protein accounting for the total was approximately 11. 80 %. the peptide was synthesized according to the sequence of grb - ast7. since ast7 is a hapten, it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc )

    根據ast _ 7氨基酸序列合成小肽，用edc做偶聯劑把合成的半抗原小肽與載體bsa偶聯成完全抗原，免疫家兔三次后，采血收集抗血清，用elisa測定效價為1 400 。
  3. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  4. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  5. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  6. Dna fragments containing rst - 0x1 and rst - 0x2 as well as their promoters were abtained by pcr. the results of sequencing and blast analysis suggested these fragments shares 99 % identity with sinorhizobium meliloti 1021. function of rst - oxl was unknown, while rst - 0x2 shares 81 % identity with gshb gene of rhizobium tropici, which encoding glutathione synthetase

    序列測定和blast分析表明，它們和苜蓿中華根瘤菌1021的相應基因有99同源性， 042bm的rst - 0x1基因功能未知，而rst - 0x2基因和熱帶根瘤菌谷胱甘肽合成酶基因gshb有81同源性。
  7. In this article, the author amplified dna sequences encoding the mature peptides of human intact and truncated igf - 1 gene from human genomic dna by the new way of alternative pcr established and named as exons - piecing together method by this laboratory. the igf - 1 and des ( l - 3 ) igf - l gene were cloned in pgem - t vector and then subcloned in expression plasmid pgex - 3x. two recombinant constructs known as pgex - 3x - igf - l and pgex - 3x - des ( l - 3 ) igf - l were transformed to e. coli dh5 a respectively

    我們利用外顯子拼接法從人的基因組dna中擴增了編碼完整型及截短型人igf - 1基因成熟肽的dna片段，並將其克隆至pgem - t載體中；經測序證明正確后，又構建了表達載體pgex - 3x - igf - 1及pgex - 3x - des （ 1 - 3 ） igf - 1 ，在大腸桿菌中進行了表達研究。
  8. By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq

    然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。
  9. On structural parameterization and functional prediction of antigenic polypeptide sequences

    抗原多肽序列分子結構表達及生物功能預測
  10. Computer - assisted analysis revealed there was a signal peptide near the n - terminal of this protein

    軟體預測0rf132有一個信號肽序列（ signalpeptide ） ，最可能的切割位點在和r20之間。
  11. Nucleotide sequencing revealed that the gd gene contained an open reading frame of 1203 bp encoding a 400 aa protein

    基因測序的結果表明gd基因包含一個1203bp的開放性閱讀框（ orf ） ，編碼400個氨基酸組成的多肽。
  12. The constructed vector was identified by sequencing. after induction by iptg, four polypeptides coding epitope expressed, respectively. research in this dissertation laid foundation for the development of nd - epitope - vaccine

    經測序鑒定與原序列沒有突變及移碼后，經iptg誘導，有四段含表位的多肽獲得了表達。
  13. The quickly developing techniques of biological mass spectrometry ( bio - ms ) in recent years realized the high throughput identification of proteins by determining the accurate mass values of trypsin - digested peptides and the randomly selected peptide sequence tags, and have been successfully used in the studies of protein interactions and post - translational modification such as the phosphorylation

    摘要近幾年快速發展起來的生物質譜技術，依靠（酶解后肽段）精確質量數測定和隨機肽序列標簽分析，實現了對蛋白質高通量的鑒定，並被成功地用於蛋白質相互作用和蛋白質磷酸化等翻譯后修飾研究。
  14. In this study, a new approach of plant virus - resistance was expored by making up the cdna of ppiv. nib gene was amplified from plasmid pysr, which is plant expression vector containing potyvirus y nib gene. the nib gene was fused with the fragment encoding the mature part of papaya proteinase iv

    將番木瓜蛋白酶的cdna序列以全長、去除信號肽部分和成熟酶部分分別連接到細菌表達載體中，表達結果表明，只有含去除信號肽部分cdna的載體才能檢測到相應的表達的蛋白條帶。
  15. Cloning and sequence of mature peptiede of human bone morphogenetic protein7cdna

    7成熟肽基因克隆及序列測定
  16. By cheeking the transformed bacterial colonies, we had filtrated the masculine clone successfully. the sequencing result showed that the inserting fragment contained intact coding sequences of dh. pban ( pheromone biosynthesis activating neuropeptide ) and three sgnps ( suboesophageal ganglion neuropeptide ) and the leading frame of it was correct

    測序結果表明，重組載體的插入片段中含有dh 、性信息素生物合成激活肽（ pheromonebiosynthesisactivatingneuropeptide ， pban ）及三個食道下神經節肽（ suboesophagealganglionneuropeptide ， sgnp ）的完整的編碼序列，且其閱讀框架（ readingframe ）完全正確。
  17. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  18. It showed two results. one is that this toxin contains two proteins, the molecular weight of these proteins are similar to each other. the other result is that hntxa is a protein which has two peptide chains

    對該毒素進行蛋白質n端測序，結果發現該毒素有兩個n端，這表明該毒素或者是一個不純的物質，它由兩個分子量近似的蛋白組成；或者是一個由兩條肽鏈組成的蛋白。
  19. After 3 rounds of screening, 10 of 20 clones were identified as positive clones and all the 10 phage clones sharing the same amino acid sequence : ehmaltypfrpp, and these positive 12mer phage clone peptide can bind with tnfa specifically. the results suggests that this method is very specific and simple for screening of binding epitope to small peptide molecule from phage display peptide library

    在以噬菌體環七肽tnfa模擬肽克隆刁k naqsoc ）為靶對噬菌體隨機十二肽庫進行h輪篩選后，挑取20個克隆，經elisa鑒定有10個克隆均與t 』 nfa結合，測序結果顯示它們均為同一氨基酸序列： ehmaltypfrpp 。
  20. The result of sequencing analysis shows that the coding region of the ba - dfe mature peptide has 825bp in length and encodes 275 amino acid residues, which has a n - terminal amino acid sequence identical to that of the purified ba - dfe determined by edman method, indicating that the cloned fragment was indeed as expected

    測序結果發現，其成熟肽編碼區有825bp ，編碼275個氨基酸殘基，推導的n -端序列與測得的該酶n -端序列完全一致，證明了m川人學博：學位論文該片段確實是ba dfe成熟肽編碼區。
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