胚區定位 的英文怎麼說
中文拼音 [pēiqūdìngwèi]
胚區定位
英文
germinal localization-
In our studies, we have used the nuclear localization signal department of medical genetics and development biology 4 ( nls ) selection system to isolate a novel gene from a mouse embryonic cdna library. the full - length cdna is about 1802bp and contains an open reading frame of 1296 nucleotides. it encodes 431 amino acid
本研究工作，利用本室構建的核定位信號篩選系統從小鼠胚胎cdna文庫中克隆到一個新的全長cdna片段，分析表明在其1802bp育回軍區大學刃士學位論文的序列中含有一個長1293hp的開放閱讀框，編碼431個氨基酸。This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。From middle blast transition, cortatin location dramatically changed to the cell cortex and there were no signals detected in cytoplasm anymore
從中期囊胚轉換（ middleblastuatransition ， mbt ）開始，皮層蛋白的亞細胞定位集中於細胞的皮層區域，其餘部分細胞質中不再出現染色。
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