胚成纖維細胞 的英文怎麼說
中文拼音 [pēichéngxiānwéixìbāo]
胚成纖維細胞
英文
mdef- 胚 : 名詞[生物學] (初期發育的生物體) embryo
- 成 : Ⅰ動詞1 (完成; 成功) accomplish; succeed 2 (成為; 變為) become; turn into 3 (成全) help comp...
- 纖 : 纖形容詞(細小) fine; minute
- 維 : Ⅰ動詞1 (連接) tie up; hold together; link 2 (保持; 保全) maintain; safeguard; preserve; keep ...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 纖維 : fibre; staple; filamentary
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。There is no different between the fibroblast feeder of mouse and the fibroblast feeder of goat according to blastoysts attached and icm proliferation. es - like cell develop embryoid body - like structure in suspension culture in vitro. es - like cells of goat differentiate into various kinds cell, egg : fibroblast cell, neurolike cell
Es細胞在體外懸浮培養,形成類胚體,山羊類es細胞在無飼養層鋪有明膠的皿底上培養,可分化為多種類型細胞,如上皮細胞、成纖維細胞、神經細胞等。Cultivation of avian encephalomyelitis virus in cef
利用雞胚成纖維細胞培養禽腦脊髓炎病毒的研究Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。As a member of this superfamily, human acidic fibroblast growth factor ( afgf or fgf1 ), expressed by a variety of cells from all three germ layers, shows great potential for clinical applications for therapy of a variety of diseases, such as parkinson ' s disease, spinal cord contusion injury, neural regeneration in reimplantation of broken - off finger, brain ischemia, renal ischemia, myocardial infarction, occlusive vascularitis, retinal ischemia, gastric ulcer and nonhealing wound and so on
人類酸性成纖維細胞生長因子( afgf , fgf - 1 )是成纖維細胞生長因子超家族成員。來自於三個胚層的多種細胞都可以表達afgf 。 afgf在治療帕金森綜合癥、急性脊柱扭曲性損傷、斷指中神經功能重建、腦缺血、腎缺血、心肌梗塞、閉塞性脈管炎、視網膜缺血、胃潰瘍及難愈合性傷口等多種臨床應用方面具有巨大潛力。Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection
亞群禽白血病病毒的雞胚成纖維細胞系建立( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining
( 3 )人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞( primordialgermcells , pgcs ) ,培養在添加人重組白血病抑制因子( lif ) 、人重組堿性成纖維細胞生長因子( bfgf )和forskolin的小鼠飼養層細胞上。The research of relationship between the kidney in the sense of tcm and mesoderm from the influence of tonifying the kidney on apoptosis of fibroblast cells of kidney - deficient rats
從補腎對腎虛大鼠成纖維細胞凋亡的影響看中醫腎與中胚層的相關性At the same time, growth factors have relation to genesis of tumor. fibroblast growth factor ( fgf ) can promote proliferation of tissues derived from mesoderm or neuroectoderm and many tissues and cells can secrete fgf
成纖維細胞生長因子( fgf )能夠誘導中胚層和神經外胚層來源細胞的增殖和分化,許多組織和細胞都可以分泌fgf , fgf可以促進多種細胞的生長和分化。The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body
小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑,其制備、傳代及冷凍保存均有不同的研究報道,飼養層的質量受許多因素的影響,如動物的品種、培養液、所傳代數及實驗條件等,關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少,故本實驗以昆明小白鼠為實驗動物,研究其胚體培養過程中細胞的形態學、組織學等方面的變化,以期為本實驗室建立飼養層細胞庫提供理論依據,同時探討心肌細胞和成纖維細胞層共培養的可行性,以期為心臟生物學特性的體外研究奠定基礎。Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast
66弱毒株在雞胚成纖維細胞上的培養Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ,分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集,其餘胚胎的染色體則處于原核期;而只在融合后3h , 27 . 9 %重構胚被標記出組裝的紡錘體,且其中的同源染色體己經分離。( 2 ) the ratio of nucleus to cytoplasm about fibroblast increased gradually with culturing time in the same generation
( 2 )體外培養的原代小鼠胚體成纖維細胞隨培養時間的增加,核質比逐漸增加。( 3 ) murine embryonic body fibroblast cells could be refrigerated well by method of embryonic freezing. the cells motility thawed was 0. 86
( 3 )胚胎冷凍法可以使成纖維細胞取得較好的冷凍保存效果,解凍活率最高可達0 . 86 。The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin
從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞,經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上,培養基中添加了lif , bfgef , foskolin等細胞因子,此後大約每7天左右傳代一次。Protective effect of emodin on wi - 38 cell toxicity induced by cisplatin
大黃素對順鉑所致人胚肺成纖維細胞毒性的作用Effect of dapk carboxylic terminal peptide on tnf - inhibition of human embryonic lung fibroblast proliferation
抑制人胚肺成纖維細胞生長的影響( 2 ) so2 derivatives has significant toxicity on human embryo lung fibroblasts, the lc50 of 4h and 24h exposure is 1. 1133 and 2. 0070mmol / l respectively
( 2 )二氧化硫代謝衍生物對人胚肺成纖維細胞具有明顯的細胞毒性作用, 4小時和24小時細胞半數存活抑制濃度lcs 。分享友人