胚胎發生培養 的英文怎麼說
中文拼音 [pēitāifāshēngpéiyǎng]
胚胎發生培養
英文
embryogenic culture- 胚 : 名詞[生物學] (初期發育的生物體) embryo
- 胎 : 名詞1 (幼體) foetus; embryo 2 (懷孕或生育的次數) birth 3 (衣服、被褥等的面子和裡子之間的襯物...
- 發 : 名詞(頭發) hair
- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 胚胎 : [生物學] embryo; embryon胚胎病理學 embryopathology; 胚胎發生 embryogeny; embryogenesis; 胚胎發育 ...
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The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 (含2既的小牛血清,常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式(例如,從0一一6 ,凍存速度為一0 . 05 / min ,再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養,解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁,但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed
摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此,人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。Furthermore the expressions of all the genes in a representative sample were examed by the recently developed method of hybridization to cdna arrays. this was intended to strengthen the theoretical background for the screening of norway spruce genotypes with low lignin content. the calli of the transformed sublines a78 - 3, a78 - 4, a78 - 5 and the untransformed control a95 : 88 : 22 were successfully induced to form mature embryos from which plantlets were established
以轉基因亞系a78 - 3 、 a78 - 4 、 a78 - 5和未轉基因對照a95 : 88 : 22的細胞愈傷組織為實驗材料,以dkm和lp - m熟化培養基培養五周后,再以1 4sh萌發培養基培養四周,成功地誘導形成了胚胎,並再生成新的小植株,萌發成活率達到80 。Techniques in embryo technology ( such as in vitro production of embryos and animal cloning ) need large quantities of high quality oocytes. but the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. it is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo
目前胚胎工程技術研究和開發(如體外生產胚胎和體細胞克隆等)需要大量高質量的成熟卵母細胞,但利用屠宰動物卵巢卵母細胞經過體外成熟培養而獲取的卵母細胞質量還遠不如體內成熟卵母細胞,其原因一般認為是由於缺乏體內主卵泡階段的獲能作用。No production by mouse embryos embryos were cultured in hanks balanced salt solution for 4 hours. then the culture medium was collected, and equal amount of griess reagent was added into it. no concentrations were determined indirectly by spectrophotometry
胚胎發育過程中no的生成胚胎在hanks液中培養4小時后,取培養液加入等體積的griess試劑,用分光光度法檢測培養液中亞硝酸鹽的濃度,依此得知no的生成。In vitro infertilization - embryo transfer ( ivf - et ) development up to now, there are many new breakthroughs in coh ( controlled ovarian hyperstimulation ), the embryo culture technique and improvement of the culture medium, but how to increase the lower embryo implantation rate, clinical pregnancy rate, and reduce multiple pregnancy rate to perplex to reproduction medicine worker always
前言體外授精?胚胎移植技術( invitroinferfilizafion - embryotransferivf - et )發展至今,在促排卵方案,胚胎培養技術及培養液的改進方面有了許多新的突破,但如何提高較低的胚胎種植率及臨床妊娠率,始終困擾著生殖醫學工作者。Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ,分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集,其餘胚胎的染色體則處于原核期;而只在融合后3h , 27 . 9 %重構胚被標記出組裝的紡錘體,且其中的同源染色體己經分離。In this paper, the progress of isolated microspore culture was reviewed : the major factors affecting regeneration ; the methods of reduplication ; the major factors affecting the formation of embryo, for example, experimental material, culture medium, raise condition, a phase of microspore growth
通過對影響大白菜游離小孢子胚胎發生的主要因素材料基因型、供體母株生長狀態、小孢子發育時期、培養方法、培養條件和影響植株再生頻率的因素以及小孢子植株的加倍方法等進行了綜述。Furthermore, the scientists were also able to grow progenitor blood cells in culture from uniparental es cells, and upon transplant into irradiated adult mice, show that these cells contribute, long - term, to the function of their hematopoietic system
此外,科學家還能夠利用單系胚胎幹細胞在培養基中培育血液祖細胞,在移植入輻射照射的成年小鼠后,發現這些細胞對造血系統功能產生長期的作用。2 establishment of genetic transformation system by agrobacterium rhizogenes the different types of explants ( hypocotyls, cotyledon, leaf blade, petiole ) were infected by strain r1601 of agrobacterium rhizogenes
( 2 0mg l )上培養30d后經過體細胞胚胎發生途徑產生大量的叢生苗;子葉培養在誘導子葉產生叢生苗培養基( msoc ) ms ba ( 2 0 。The expression patterns of soluble proteins, peroxidase and esterase isozymes were analysed on globular embryogenesis in tissue cultures of alfalfa, it was concluded that from embryogenic callus to globular embryo genesis h peptides emerged and disappeared orderly, the patterns of peroxidase isozyme changed dramatically, esterase isozymes changed slightly while the total enzyme activity was important to keeping the potential of somatic embryogenesis
摘要試驗在苜蓿組織培養中,對球形胚形成過程中特異蛋白質表達的模式、過氧化物酶及酯酶同工酶酶譜變化進行研究,結果表明:苜蓿組織培養中從胚性愈傷組織到球形胚發育的進程中,順序消失和出現了11種中小分子量多肽;過氧化物酶同工酶酶譜發生了顯著的變化;酯酶同工酶酶譜變化不大,但其總活力對于維持體細胞胚胎發生是必須的。Embryonic stem ( es ) cells are pluripotent cells derived from the inner cell mass ( icm ) of early blastrocyts, which possess the ability of unlimitted proliferation and maintain normal dipoid keptypes under differentiation - inhibited culture in vitro, they will differentiate into multiple lineage cell types or occur commentent and differentiation without differentiation - inhibited factors or hi presence of certain induced - factors.
胚胎幹細胞( es細胞) ,是存在於早期胚胎內細胞團( icm )的一種多潛能細胞。在體外抑制分化培養時, es細胞可無限增殖並保持正常的二倍體核型,一旦撤除分化抑制因素,或添加適當誘導劑, es細胞可發生譜系分化或定向分化。分享友人