胚胎發育率 的英文怎麼說
中文拼音 [pēitāifāyùlǜ]
胚胎發育率
英文
embryo development rate- 胚 : 名詞[生物學] (初期發育的生物體) embryo
- 胎 : 名詞1 (幼體) foetus; embryo 2 (懷孕或生育的次數) birth 3 (衣服、被褥等的面子和裡子之間的襯物...
- 發 : 名詞(頭發) hair
- 育 : 育動詞1. (生育) give birth to 2. (養活; 培育) rear; raise; bring up 3. (教育) educate
- 率 : 率名詞(比值) rate; ratio; proportion
- 胚胎 : [生物學] embryo; embryon胚胎病理學 embryopathology; 胚胎發生 embryogeny; embryogenesis; 胚胎發育 ...
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According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere
摘要從哺乳動物早期胚胎體外培養發育阻斷機理和早期胚胎的體內環境入手,闡述了胚胎體外培養各細胞階段胚胎對營養物質的需求,尋求合理的體外培養條件和方法,以便提高體外胚胎早期的囊胚發育率。The protein nucleic acid complexes were identified by autoradiography for 48 h. the psg5heo and igfbp - 6 promoter pcat vectors were transfected into cos - 7 cells with iipofectamin2000 ; these cos - 7 cells where treated with 1 jxm e2, with lonm tcdd and with both lonm tcdd and 1 jxm e2, individually ; the cat protein was examined by cat - els a. results : the congenital skeletal defects in fetal rats were induced with 10 - 15ug / kg tcdd or with 100 - 140 mg / kg ra with dosage - dependence effect. the igfbp - 6 mrna in rat calvaria tissue and mc - 3t3 - el cells were increased with tcdd and ( or ) ra, whereas the cell proliferation and igf - 2 mrna in mc - 3t3 - el cells was decreased in the status of e2
結果: tcdd在大鼠胚胎發育時期具有骨骼致畸作用,骨骼發育畸形包括:內翻足、脊柱裂、顱面骨發育缺陷、無尾畸形等;隨著tcdd濃度的升高( 10一15林擴kg ) ,致畸率明顯上升( 14 . 7 %一24 . 6 % ) ; tcdd和ra聯合用藥可以增強對大鼠骨骼發育的致畸效應; e :對成骨細胞內igf一2基因有正性調節作用,對igfbp一6基因有負性調節作用, tcdd可以抑制e :對細胞內xgf一2和igfbp一6的調節作用。Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。In the 2 - step ops method, embryos were pretreated with 10 % eg + 10 % dmso for 30s before exposed to edfs30, a vitrification solution, for 25s, then immersed in liquid nitrogen. the blastocyst rate of vitrified mouse morula after equilibration in 0. 5mol / l sucrose for 5min was 100 %
Ops二步法即胚胎預處理30s ,然後移入edfs30冷凍液中平衡25s冷凍保存,解凍后在0 . 5mol l蔗糖溶液中平衡5min ,培養后囊胚發育率為( 100 ) 。Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %
採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵,實驗結果如下: ( 1 )顯微注射后,根據胚胎發育分期,胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光,原腸期發熒光的胚胎比例為25 ,後期發育熒光率逐漸下降,肌肉效應期后又相對穩定。The results show that the development rates of morula treated with serum starvation for 3d, 4d, 5d are higher apparently than nocodazole group and control group ( p < 0. 05 )
直接注射法構建的核移植重組胚胎的桑堪胚發育率( 25 95 )高於電融合法( 16 07 ) ,統計學分析差異顯著( p 0These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells, and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes, and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed. the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses
以上結果顯示, 6 dmap能增加胚胎幹細胞異種重構卵的卵裂率,對重構卵囊胚的發育率影響不大;高培養代數和低培養代數的胚胎幹細胞用於異種核移植不影響異種重構卵的卵裂率和囊胚發育率;用胚胎幹細胞作為供核細胞;比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程,並且胚胎于細胞指導異種克隆胚胎正常發育的能力強于體細胞。Obesity, pre - diabetes and diabetes can affect ejaculation, sperm production, ovulation, miscarriage rates and increase fetal malformation rates
肥胖、糖尿病前期和糖尿病都能影響射精、精子產生、排卵、流產幾率和增加胚胎發育不良的機會。In this paper, the progress of isolated microspore culture was reviewed : the major factors affecting regeneration ; the methods of reduplication ; the major factors affecting the formation of embryo, for example, experimental material, culture medium, raise condition, a phase of microspore growth
通過對影響大白菜游離小孢子胚胎發生的主要因素材料基因型、供體母株生長狀態、小孢子發育時期、培養方法、培養條件和影響植株再生頻率的因素以及小孢子植株的加倍方法等進行了綜述。Meanwhile, oxygen concentration of culture medium containing embryos decreased with time ( p < 0. 0001 ). with the development of embryos, oxygen exhausting rate trended to increase
未受精卵耗氧極低,而胚胎培養液中氧的濃度直線下降( p 0 . 0001 ) ,而且隨著胚胎的發育耗氧率有上升的趨勢。Recovery of microtubule organizing centers in enucleated oocytes and condensation of chromosome and organization of spindle in reconstructed embryos were analysed. a non - invasive method of enucleation mouse oocytes has been developed. firstly, the progression of mouse oocytes in meitosis i was ascertained by immunofluoresence technique
另外,本試驗通過在m16基礎上添加eaa 、 non - eaa和葡萄糖顯著提高了胚胎的桑椹率、囊胚率和孵化率;單獨添加葡萄糖的培養液,其桑椹率顯著升高,但囊胚率和孵化率的升高並不顯著,所以胚胎培養液中氨基酸和葡萄糖能協同促進胚胎發育。The technique of microinjection is significant to embryo ' s development. for example, the different volume of microinjection will have direct effect on the in vitro survival rate of the embryos and even the successful rate of the transgenic animals
顯微注射技術對胚胎發育有著關鍵性的影響,如注射劑量的不同將直接影響到胚胎體外發育的存活率乃至影響整個轉基因動物的成功率。Mouse blastocysts and morulas were cultured in rat heart conditioned medium ( rh - cm ). as far as the following indexes attaching ratio, icm growth ratio and es clone formation ratio are concerned, the choice of blastocysts is much better than morulas ( p < 0. 05 ), therefore mouse blastocysts were more suitable than morulas to get es cells
源於icr小鼠icm的es細胞分離克隆研究試驗以rh - cm為培養基培養處于不同發育階段的小鼠胚胎,發現由囊胚分離的es細胞傳代后es集落的出現率顯著高於桑椹胚( p 0 . 05 ) ,故囊胚更適合作為es細胞分離克隆的材料。The enucleated rates can reach at 87. 06 %. 3 ) three activated methods may effectively activate the black bear - cat reconstructed embryos. whereas activated effect of ethanol + 6 - dmap + ccb is more effective, the cleavage rate and the development rate to m
重組胚在3種培養液中的發育能力有差異t 0刀引, m199成熟培養液比dmem12 、 m16更能支待重組胚胎的卵裂和桑棋胚發育,卵裂率和桑堪胚發育率分別為63分享友人