胞外區 的英文怎麼說

中文拼音 [bāowàiqū]
胞外區 英文
extracellular region
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 外 : Ⅰ名詞1 （外面） outside; external side 2 （外國） foreign country 3 （以外） besides; beyond; in ...
  • 區 : 區名詞（姓氏） a surname
  1. Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome

    為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。
  2. Massive amounts of extracellular fluid may be sequestered in the area of peritoneal injury.

    大量細胞外液滯留于腹膜損傷區。
  3. Inspection for the biological activities of recombination protein of ? ctla4 extracellular domain in vitro : we investigated the role of ctla4e in t cell proliferation of mlr with 3h - tdr incorporation

    重組人ctla4胞外區蛋白體內生物學活性研究： l ）觀察ctla4胞外區蛋白在小鼠變應性鼻炎中的作用。
  4. Hence, yeast gs115 was used to express the target protein in this study. objection : to obtain the biological activated recombianated protein of huaman ctla4 extracellular domain ( ctla4e ), and to explore its effects on allergic diseases and transplant rejection

    目的：獲取具有生物學活性的重組人ctla4胞外區蛋白（ ctla4e ） ，探討其在變應性疾病和器官移植排斥反應防治中的作用和機理。
  5. The stable expression yeast was obtained by screening and was induced to ferment by methanol

    將ppicgk七質粒電轉化gslls酵母；通過篩選獲取穩定表達ctla4胞外區蛋白的表達菌。
  6. Thereof the ten - peptide is chemically synthesized, and its apoptosis - inducing activity on tumor cells bearing fas is investigated in parallel with recombinant excellular domain of fasl ( fasl - ecd )

    化學合成了十肽結構，與重組fasl - ecd （ fasl胞外區）平行對照，研究了其對培養的腫瘤細胞的相互作用。
  7. The recombinant fasl - ecd was purified and its biological activity was analyzed on several cell lines and the most sensitive cell lines are selected. ( 2 ) using a computer program, a single short peptide is derived from antisense homology box of fas ligand and is chemically synthesized. ( 3 ) examine the apoptosis - inducing effect of the recombinant fasl - ecd and the ten - peptide on the most sensitive cell lines, and the relationship between them was analysed

    純化重組蛋白fasl - ecd ，並分析其生物學活性，篩選出對之最敏感的腫瘤細胞株； （ 2 ）根據生物信息學軟體分析結果，選取fasl胞外區256 - 265的十肽（ n ） - hlyvnvsels - （ c ）作為目標分子，委託生物公司合成該十肽； （ 3 ）分析fasl - ecd和十肽對最敏感腫瘤細胞的毒性作用，分析重組fasl一ecd及十膚作用的相關性。
  8. After ultrafiltration and rough separation with ammonium sulfate and anion - exchange chromatography, the recombination protein of ctla4 extracelluar domain was rectified from the ferment supernatants

    表達菌甲醇誘導發酵；發酵上清液經超濾、硫酸胺粗分級分離以及陰離于交換層析，純化出ctla4胞外區蛋白。
  9. We found that insulin - degrading enzyme ( ide ) interacts with ge through its extracellular domain

    我們發現胰島素降解酶（ ide ）可通過其細胞外區域與糖蛋白e相互作用。
  10. Other domains of the extracellular part of bt - r3 protein are 11 cadherin repeated domains, which mainly consist of p stands that are linked by loop structure, turn residues and random coil

    其餘的胞外區域形成以折疊為主的11個鈣粘蛋白重復結構域。這些d折疊又由loop結構、轉角和無規捲曲連結在一起。
  11. Cloning and sequencing of human cbfa1 cdna fragment from osteosarcoma cell

    胞外區片段的克隆和序列分析
  12. Cloning of extracellular domain fragment of human light gene and expression of fusion protein

    胞外區基因的克隆及融合蛋白的表達
  13. The expression of target protein was identified with dot - elisa, sds - page and western blotting

    Elisa 、 sda page 、 western blot等方法在蛋白水平對ctla4胞外區蛋白的表達進行了鑒定。
  14. Meanwhile, because the fragment of ctla4 extracellular domain is only 400bp, its expression will be more stable than that of ctlaig fusion protein ( 1. 2kb )

    同時，由於ctla4胞外區片段大小僅有400hp ，較ctla4ig融合蛋白zkb ）在表達菌中的表達將更為穩定。
  15. The present investigation is favorable for screening the new generation of anti - tumor peptides. [ objective ] ( 1 ) to clone and express fasl - ecd in e. coli

    [目的] （ 1 ）分析人黑色素瘤細胞（ a375 ）中fasl基因的表達，克隆人fasl胞外區的編碼基因，並在大腸桿菌bl21中進行表達。
  16. The cdna of hn gene from b95 strain, shares a high identity between 88 % and 95. 1 % with that of reported hn genes. the amino acid residues predicted, shares an identity between 92. 1 % and 96. 1 % with that of other strains

    1 ，氨基酸的差異主要集中在n端18 75位。該范圍內包括hn蛋白胞質區、跨膜區和細胞外區。這些變異對hn蛋白生物活性的影響還有待進一步研究。
  17. Fasl - ecd coding sequence is subcloned into pet - lla expressing vector, recombinant expression vector are named as pet - fasl - ecd. this plasmid is introduced into e. coli bl21. after induction with 1mmol / l iptg, the protein expression is analyzed and confirmed by coomassie - stained sds - page

    用pcr和酶切鑒定的方法篩選出陽性重組子，將陽性重組子以ilnlnol幾iptg進行誘導表達，以sds一page分析fasl胞外區的表達。
  18. [ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ), full length fasl gene is detected by rt - pcr. using the cdna as template, . the extracellular domain of fasl ( fasl - ecd, 127 - 278aa ) is amplified by pcr. the pcr products are directly cloned into t vector pmd - 18t

    L方法採用新鮮人黑色素瘤細胞（ a375 ） ，抽提該細胞的總rna ，進行rt一pcr反應分析a375內fasl全長編碼基因的轉錄表達，以a375細胞cdna為模板，用pcr產物直接克隆法擴增人fasl一ecd （人fasl胞外區）的編碼基因，即127一278位氨基酸殘基，而後將pcr產物直接克隆于pmd一1st載體中，獲得重組質粒pmd一t - fasl一ecd ，進行dna測序。
  19. Methods : 1. expression and purification of recombinant human ctla4 extracellular domain : a 400bp dna fragment of ctla4 extracellular domain was obtained from the pctla4 / ig plasmid with genetical technique. then, this dna fragment was inserted into ppic9 plasmid to construct the single copy plasmid of yeast expression system ( ppic9 - ctla4125 ). furthermore, the multi - copy plasmid ( ppic9k - ctla4125 ) was constructed

    重組人ctla4胞外區蛋白在酵母gs115中的表達和純化：首先通過基因操作技術，從pctla4八g質粒中擴增400hp的ctla4胞外區片段；將ctla4胞外區片段插人ppicg質粒中獲取單拷貝酵母表達質粒ppicg ctla4125並進一步構建多拷貝酵母表達質粒ppicgk （ 。
  20. Ctla4ig fusion protein was expressed by genetically fusing the dna fragment encoding 1 - 125 amino acid residues of ctla4 extracellular domain with dna fragment encoding an igg fc region. its in functional region is the extracellular domain of ctla4

    Ctla4ig融合蛋白是通過基因重組技術將編碼ctla4胞外區1 - 125個氨基酸序列的dna片段與編碼iggfc段的dna片段融合后表達生成，其功能區是ctla4胞外區。
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