胰蛋白酶處理 的英文怎麼說

中文拼音 [yídànbáichǔlǐ]
胰蛋白酶處理 英文
trypsinization
  • 胰 : 名詞[生理學] （胰腺） pancreas
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 處 : 處名詞1 （地方） place 2 （方面; 某一點） part; point 3 （機關或機關里一個部門） department; offi...
  • 理 : Ⅰ名詞1 （物質組織的條紋） texture; grain （in wood skin etc ） 2 （道理;事理） reason; logic; tru...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  2. In this study, one strain of avian rotavirus was isolated from a chicken with natural diarrthoea. isolation and cultivation of the rotavirus was attemped by the inoculating on monolayers of marc 145 cells with a suspension of faecal material containing the rotavirus

    本文對發生在山東某地雞流行性腹瀉病例的病原進行了研究。用胰蛋白酶處理病雞的糞便和腸內容物，接種marc145細胞，盲傳數代後分離到一株病毒。
  3. During the culture of the porcine ear skin fibroblasts, the successful rate was increased by selecting culture methods, contrasting donor age and improving culture condition. the rate of living cells in the method of combining trypsin cold treatment with trypsin heat treatment was much higher than that in the method of trypsin heat treatment. the results showed that the method of combining trypsin cold treatment with trypsin heat treatment had less adverse effects on cells than the method of trypsin heat treatment

    在培養豬耳皮膚成纖維細胞時從篩選細胞分離方法、比較供體年齡和改善培養條件這三方面著手提高培養的成功率。實驗中發現胰蛋白酶冷熱處理結合法培養的細胞存活率明顯高於胰蛋白酶熱處理法，說明使用胰蛋白酶冷熱處理結合法對細胞的不利影響較少，細胞產量較高。
  4. Then, the sensitivity of the subunits to trypsin traetment was compared by polypeptide pattern among these five psii preparations with sds - page

    分別用不同濃度的胰蛋白酶對這5種ps制劑進行限制性蛋白酶解處理， sds - page分析多肽組分變化。
  5. 2 ) hanks " buffer of trypsin not being added edta could obtain better the growth potential and the subsequent attachment of harvested skin cells t then it being added edta have better digested potent

    與添加edta相比，用只含胰蛋白酶的hanks '液處理貼壁細胞，所回收的傳代細胞存活能力較好；而添加edta的胰蛋白酶消化液消化能力較強。
  6. Both xyna and xynb did n ' t display cellulase activity both xyna and xynb were treated by pepsin or trypsin for 30 min under 37c, their activity were n ' t decreased

    胃蛋白酶、胰蛋白酶37下處理30分鐘，對酶活性無影響。將純化出的xyna及xynb進行n端序列測定。
  7. The lhcii, which lies outside, was digested partly in both naci - washed psil particle binding the 33 kd protein and caci2 - washed psil particle lacking the 33 kd protein, independent of binding of 33 kd protein. however, caci2 - washed psil particle was more sensitive to tryptic attack than naci - washed psil particle, especially, cp43 decreased more significantly in caci2 - washed psil particle than in naci - washed psil particle. oxygen - evolving psil core complex was sensitive to trypsin digesting, cp43, d2, d1 and 33 kd protein were digested even under slight trypsin treatment and the fragment were verified by western blotting

    結果顯示：在nacl鹽洗ps顆粒和cacl _ 2處理ps顆粒中， lhc容易被酶解； cacl _ 2處理ps顆粒比nacl鹽洗ps顆粒對胰蛋白酶作用敏感；與nacl鹽洗ps顆粒相比，在除去33kd蛋白后， cacl _ 2處理ps顆粒的cp43更容易被酶解；放氧核心復合物對胰蛋白酶更敏感，在低濃度的胰蛋白酶作用下， cp47不被水解，而cp43 、 d _ 2 、 d _ 1和33kd蛋白被部分水解， western - blotting可以檢測到它們的水解片段。
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